http://purl.uniprot.org/citations/28612031 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/28612031 | http://www.w3.org/2000/01/rdf-schema#comment | "ObjectiveDecreased expression of collagen type II in favour of collagen type I or X is one hallmark of chondrocyte phenotype changes in osteoarthritic (OA) cartilage. MicroRNA-(miR-) 29b was previously shown to target collagens in several tissues. We studied whether it could contribute to collagen imbalance in chondrocytes with an impaired phenotype.MethodsAfter preliminary microarrays screening, miR-29b levels were measured by RT-quantitative PCR in in vitro models of chondrocyte phenotype changes (IL-1β challenge or serial subculturing) and in chondrocytes from OA and non-OA patients. Potential miR-29b targets identified in silico in 3'-UTRs of collagens mRNAs were tested with luciferase reporter assays. The impact of premiR-29b overexpression in ATDC5 cells was studied on collagen mRNA levels and synthesis (Sirius red staining) during chondrogenesis.ResultsMiR-29b level increased significantly in IL-1β-stimulated and weakly in subcultured chondrocytes. A 5.8-fold increase was observed in chondrocytes from OA versus non-OA patients. Reporter assays showed that miR-29b targeted COL2A1 and COL1A2 3'-UTRs although with a variable recovery upon mutation. In ATDC5 cells overexpressing premiR-29b, collagen production was reduced while mRNA levels increased.ConclusionsBy acting probably as a posttranscriptional regulator with a different efficacy on COL2A1 and COL1A2 expression, miR-29b can contribute to the collagens imbalance associated with an abnormal chondrocyte phenotype."xsd:string |
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http://purl.uniprot.org/citations/28612031 | http://purl.uniprot.org/core/author | "Branlant C."xsd:string |
http://purl.uniprot.org/citations/28612031 | http://purl.uniprot.org/core/author | "Charpentier B."xsd:string |
http://purl.uniprot.org/citations/28612031 | http://purl.uniprot.org/core/author | "Salone V."xsd:string |
http://purl.uniprot.org/citations/28612031 | http://purl.uniprot.org/core/author | "Behm-Ansmant I."xsd:string |
http://purl.uniprot.org/citations/28612031 | http://purl.uniprot.org/core/author | "Clement T."xsd:string |
http://purl.uniprot.org/citations/28612031 | http://purl.uniprot.org/core/author | "Moulin D."xsd:string |
http://purl.uniprot.org/citations/28612031 | http://purl.uniprot.org/core/author | "Jouzeau J.Y."xsd:string |
http://purl.uniprot.org/citations/28612031 | http://purl.uniprot.org/core/author | "Koufany M."xsd:string |
http://purl.uniprot.org/citations/28612031 | http://purl.uniprot.org/core/date | "2017"xsd:gYear |
http://purl.uniprot.org/citations/28612031 | http://purl.uniprot.org/core/name | "Biomed Res Int"xsd:string |
http://purl.uniprot.org/citations/28612031 | http://purl.uniprot.org/core/pages | "9792512"xsd:string |
http://purl.uniprot.org/citations/28612031 | http://purl.uniprot.org/core/title | "MicroRNA-29b Contributes to Collagens Imbalance in Human Osteoarthritic and Dedifferentiated Articular Chondrocytes."xsd:string |
http://purl.uniprot.org/citations/28612031 | http://purl.uniprot.org/core/volume | "2017"xsd:string |
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