| http://purl.uniprot.org/citations/28617811 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/28617811 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/28617811 | http://www.w3.org/2000/01/rdf-schema#comment | "Spermatogenesis is a dynamic process that is regulated by adhesive interactions between germ and Sertoli cells. Germ cells express the Junctional Adhesion Molecule-C (JAM-C, encoded by Jam3), which localizes to germ/Sertoli cell contacts. JAM-C is involved in germ cell polarity and acrosome formation. Using a proteomic approach, we demonstrated that JAM-C interacted with the Golgi reassembly stacking protein of 55 kDa (GRASP55, encoded by Gorasp2) in developing germ cells. Generation and study of Gorasp2-/-mice revealed that knock-out mice suffered from spermatogenesis defects. Acrosome formation and polarized localization of JAM-C in spermatids were altered in Gorasp2-/-mice. In addition, Golgi morphology of spermatocytes was disturbed in Gorasp2-/-mice. Crystal structures of GRASP55 in complex with JAM-C or JAM-B revealed that GRASP55 interacted via PDZ-mediated interactions with JAMs and induced a conformational change in GRASP55 with respect of its free conformation. An in silico pharmacophore approach identified a chemical compound called Graspin that inhibited PDZ-mediated interactions of GRASP55 with JAMs. Treatment of mice with Graspin hampered the polarized localization of JAM-C in spermatids, induced the premature release of spermatids and affected the Golgi morphology of meiotic spermatocytes."xsd:string |
| http://purl.uniprot.org/citations/28617811 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pgen.1006803"xsd:string |
| http://purl.uniprot.org/citations/28617811 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pgen.1006803"xsd:string |
| http://purl.uniprot.org/citations/28617811 | http://purl.uniprot.org/core/author | "Morelli X."xsd:string |
| http://purl.uniprot.org/citations/28617811 | http://purl.uniprot.org/core/author | "Morelli X."xsd:string |
| http://purl.uniprot.org/citations/28617811 | http://purl.uniprot.org/core/author | "Shi X."xsd:string |
| http://purl.uniprot.org/citations/28617811 | http://purl.uniprot.org/core/author | "Shi X."xsd:string |
| http://purl.uniprot.org/citations/28617811 | http://purl.uniprot.org/core/author | "Betzi S."xsd:string |
| http://purl.uniprot.org/citations/28617811 | http://purl.uniprot.org/core/author | "Betzi S."xsd:string |
| http://purl.uniprot.org/citations/28617811 | http://purl.uniprot.org/core/author | "Audebert S."xsd:string |
| http://purl.uniprot.org/citations/28617811 | http://purl.uniprot.org/core/author | "Audebert S."xsd:string |
| http://purl.uniprot.org/citations/28617811 | http://purl.uniprot.org/core/author | "Durand B."xsd:string |
| http://purl.uniprot.org/citations/28617811 | http://purl.uniprot.org/core/author | "Durand B."xsd:string |
| http://purl.uniprot.org/citations/28617811 | http://purl.uniprot.org/core/author | "Borg J.P."xsd:string |
| http://purl.uniprot.org/citations/28617811 | http://purl.uniprot.org/core/author | "Borg J.P."xsd:string |
| http://purl.uniprot.org/citations/28617811 | http://purl.uniprot.org/core/author | "Roche P."xsd:string |
| http://purl.uniprot.org/citations/28617811 | http://purl.uniprot.org/core/author | "Roche P."xsd:string |
| http://purl.uniprot.org/citations/28617811 | http://purl.uniprot.org/core/author | "Marchetto S."xsd:string |
| http://purl.uniprot.org/citations/28617811 | http://purl.uniprot.org/core/author | "Marchetto S."xsd:string |
| http://purl.uniprot.org/citations/28617811 | http://purl.uniprot.org/core/author | "Shi N."xsd:string |
| http://purl.uniprot.org/citations/28617811 | http://purl.uniprot.org/core/author | "Shi N."xsd:string |
| http://purl.uniprot.org/citations/28617811 | http://purl.uniprot.org/core/author | "Lembo F."xsd:string |
| http://purl.uniprot.org/citations/28617811 | http://purl.uniprot.org/core/author | "Lembo F."xsd:string |