| http://purl.uniprot.org/citations/28683086 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/28683086 | http://www.w3.org/2000/01/rdf-schema#comment | "Regulation of capsid disassembly is crucial for efficient HIV-1 cDNA synthesis after entry, yet host factors involved in this process remain largely unknown. Here, we employ genetic screening of human T-cells to identify maternal embryonic leucine zipper kinase (MELK) as a host factor required for optimal uncoating of the HIV-1 core to promote viral cDNA synthesis. Depletion of MELK inhibited HIV-1 cDNA synthesis with a concomitant delay of capsid disassembly. MELK phosphorylated Ser-149 of the capsid in the multimerized HIV-1 core, and a mutant virus carrying a phosphorylation-mimetic amino-acid substitution of Ser-149 underwent premature capsid disassembly and earlier HIV-1 cDNA synthesis, and eventually failed to enter the nucleus. Moreover, a small-molecule MELK inhibitor reduced the efficiency of HIV-1 replication in peripheral blood mononuclear cells in a dose-dependent manner. These results reveal a previously unrecognized mechanism of HIV-1 capsid disassembly and implicate MELK as a potential target for anti-HIV therapy."xsd:string |
| http://purl.uniprot.org/citations/28683086 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.ppat.1006441"xsd:string |
| http://purl.uniprot.org/citations/28683086 | http://purl.uniprot.org/core/author | "Miyamoto T."xsd:string |
| http://purl.uniprot.org/citations/28683086 | http://purl.uniprot.org/core/author | "Saito H."xsd:string |
| http://purl.uniprot.org/citations/28683086 | http://purl.uniprot.org/core/author | "Takeuchi H."xsd:string |
| http://purl.uniprot.org/citations/28683086 | http://purl.uniprot.org/core/author | "Noda T."xsd:string |
| http://purl.uniprot.org/citations/28683086 | http://purl.uniprot.org/core/author | "Yamaoka S."xsd:string |
| http://purl.uniprot.org/citations/28683086 | http://purl.uniprot.org/core/author | "Yoshinaga T."xsd:string |
| http://purl.uniprot.org/citations/28683086 | http://purl.uniprot.org/core/author | "Ishii H."xsd:string |
| http://purl.uniprot.org/citations/28683086 | http://purl.uniprot.org/core/author | "Tsunetsugu-Yokota Y."xsd:string |
| http://purl.uniprot.org/citations/28683086 | http://purl.uniprot.org/core/author | "Terahara K."xsd:string |
| http://purl.uniprot.org/citations/28683086 | http://purl.uniprot.org/core/date | "2017"xsd:gYear |
| http://purl.uniprot.org/citations/28683086 | http://purl.uniprot.org/core/name | "PLoS Pathog"xsd:string |
| http://purl.uniprot.org/citations/28683086 | http://purl.uniprot.org/core/pages | "e1006441"xsd:string |
| http://purl.uniprot.org/citations/28683086 | http://purl.uniprot.org/core/title | "Phosphorylation of the HIV-1 capsid by MELK triggers uncoating to promote viral cDNA synthesis."xsd:string |
| http://purl.uniprot.org/citations/28683086 | http://purl.uniprot.org/core/volume | "13"xsd:string |
| http://purl.uniprot.org/citations/28683086 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/28683086 |
| http://purl.uniprot.org/citations/28683086 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/28683086 |
| http://purl.uniprot.org/uniprot/#_A7BG63-mappedCitation-28683086 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28683086 |
| http://purl.uniprot.org/uniprot/#_B7Z1G6-mappedCitation-28683086 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28683086 |
| http://purl.uniprot.org/uniprot/#_B7Z450-mappedCitation-28683086 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28683086 |
| http://purl.uniprot.org/uniprot/#_Q53GX0-mappedCitation-28683086 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28683086 |
| http://purl.uniprot.org/uniprot/#_Q14680-mappedCitation-28683086 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28683086 |
| http://purl.uniprot.org/uniprot/A7BG63 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/28683086 |