| http://purl.uniprot.org/citations/28695742 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/28695742 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/28695742 | http://www.w3.org/2000/01/rdf-schema#comment | "Ki-1/57 is a nuclear and cytoplasmic regulatory protein first identified in malignant cells from Hodgkin's lymphoma. It is involved in gene expression regulation on both transcriptional and mRNA metabolism levels. Ki-1/57 belongs to the family of intrinsically unstructured proteins and undergoes phosphorylation by PKC and methylation by PRMT1. Previous characterization of its protein interaction profile by yeast two-hybrid screening showed that Ki-1/57 interacts with proteins of the SUMOylation machinery, the SUMO E2 conjugating enzyme UBC9 and the SUMO E3 ligase PIAS3, which suggested that Ki-1/57 could be involved with this process. Here we identified seven potential SUMO target sites (lysine residues) on Ki-1/57 sequence and observed that Ki-1/57 is modified by SUMO proteins in vitro and in vivo. We showed that SUMOylation of Ki-1/57 occurred on lysines 213, 276, and 336. In transfected cells expressing FLAG-Ki-1/57 wild-type, its paralog FLAG-CGI-55 wild-type, or their non-SUMOylated triple mutants, the number of PML-nuclear bodies (PML-NBs) is reduced compared with the control cells not expressing the constructs. More interestingly, after treating cells with arsenic trioxide (As2O3), the number of PML-NBs is no longer reduced when the non-SUMOylated triple mutant Ki-1/57 is expressed, suggesting that the SUMOylation of Ki-1/57 has a role in the control of As2O3-induced PML-NB formation. A proteome-wide analysis of Ki-1/57 partners in the presence of either SUMO-1 or SUMO-2 suggests that the involvement of Ki-1/57 with the regulation of gene expression is independent of the presence of either SUMO-1 or SUMO-2; however, the presence of SUMO-1 strongly influences the interaction of Ki-1/57 with proteins associated with cellular metabolism, maintenance, and cell cycle."xsd:string |
| http://purl.uniprot.org/citations/28695742 | http://purl.org/dc/terms/identifier | "doi:10.1021/acs.jproteome.7b00001"xsd:string |
| http://purl.uniprot.org/citations/28695742 | http://purl.org/dc/terms/identifier | "doi:10.1021/acs.jproteome.7b00001"xsd:string |
| http://purl.uniprot.org/citations/28695742 | http://purl.uniprot.org/core/author | "Costello C.E."xsd:string |
| http://purl.uniprot.org/citations/28695742 | http://purl.uniprot.org/core/author | "Costello C.E."xsd:string |
| http://purl.uniprot.org/citations/28695742 | http://purl.uniprot.org/core/author | "Saito A."xsd:string |
| http://purl.uniprot.org/citations/28695742 | http://purl.uniprot.org/core/author | "Saito A."xsd:string |
| http://purl.uniprot.org/citations/28695742 | http://purl.uniprot.org/core/author | "Costa F.C."xsd:string |
| http://purl.uniprot.org/citations/28695742 | http://purl.uniprot.org/core/author | "Costa F.C."xsd:string |
| http://purl.uniprot.org/citations/28695742 | http://purl.uniprot.org/core/author | "Santos M.T."xsd:string |
| http://purl.uniprot.org/citations/28695742 | http://purl.uniprot.org/core/author | "Santos M.T."xsd:string |
| http://purl.uniprot.org/citations/28695742 | http://purl.uniprot.org/core/author | "Bressan G.C."xsd:string |
| http://purl.uniprot.org/citations/28695742 | http://purl.uniprot.org/core/author | "Bressan G.C."xsd:string |
| http://purl.uniprot.org/citations/28695742 | http://purl.uniprot.org/core/author | "Kobarg J."xsd:string |
| http://purl.uniprot.org/citations/28695742 | http://purl.uniprot.org/core/author | "Kobarg J."xsd:string |
| http://purl.uniprot.org/citations/28695742 | http://purl.uniprot.org/core/author | "Goncalves K.A."xsd:string |
| http://purl.uniprot.org/citations/28695742 | http://purl.uniprot.org/core/author | "Goncalves K.A."xsd:string |
| http://purl.uniprot.org/citations/28695742 | http://purl.uniprot.org/core/author | "McComb M.E."xsd:string |
| http://purl.uniprot.org/citations/28695742 | http://purl.uniprot.org/core/author | "McComb M.E."xsd:string |
| http://purl.uniprot.org/citations/28695742 | http://purl.uniprot.org/core/author | "Meirelles G.V."xsd:string |
| http://purl.uniprot.org/citations/28695742 | http://purl.uniprot.org/core/author | "Meirelles G.V."xsd:string |
| http://purl.uniprot.org/citations/28695742 | http://purl.uniprot.org/core/author | "Souza E.E."xsd:string |
| http://purl.uniprot.org/citations/28695742 | http://purl.uniprot.org/core/author | "Souza E.E."xsd:string |