Results

RDF/XMLNTriplesTurtleShow queryShare ▶
SubjectPredicateObject
http://purl.uniprot.org/citations/28705318http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/28705318http://www.w3.org/2000/01/rdf-schema#comment"

Background

Inherited cardiac conduction disease is a rare bradyarrhythmia associated with mutations in various genes that affect action potential propagation. It is often characterized by isolated conduction disturbance of the His-Purkinje system, but it is rarely described as a syndromic form.

Objectives

The authors sought to identify the genetic defect in families with a novel bradyarrhythmia syndrome associated with bone malformation.

Methods

The authors genetically screened 15 European cases with genotype-negative de novo atrioventricular (AV) block and their parents by trio whole-exome sequencing, plus 31 Japanese cases with genotype-negative familial AV block or sick sinus syndrome by targeted exon sequencing of 457 susceptibility genes. Functional consequences of the mutation were evaluated using an in vitro cell expression system and in vivo knockout mice.

Results

The authors identified a connexin-45 (Cx45) mutation (p.R75H) in 2 unrelated families (a de novo French case and a 3-generation Japanese family) who presented with progressive AV block, which resulted in atrial standstill without ventricular conduction abnormalities. Affected individuals shared a common extracardiac phenotype: a brachyfacial pattern, finger deformity, and dental dysplasia. Mutant Cx45 expressed in Neuro-2a cells showed normal hemichannel assembly and plaque formation. However, Lucifer yellow dye transfer and gap junction conductance between cell pairs were severely impaired, which suggested that mutant Cx45 impedes gap junction communication in a dominant-negative manner. Tamoxifen-induced, cardiac-specific Cx45 knockout mice showed sinus node dysfunction and atrial arrhythmia, recapitulating the intra-atrial disturbance.

Conclusions

Altogether, the authors showed that Cx45 mutant p.R75H is responsible for a novel disease entity of progressive atrial conduction system defects associated with craniofacial and dentodigital malformation."xsd:string
http://purl.uniprot.org/citations/28705318http://purl.org/dc/terms/identifier"doi:10.1016/j.jacc.2017.05.039"xsd:string
http://purl.uniprot.org/citations/28705318http://purl.uniprot.org/core/author"Horie M."xsd:string
http://purl.uniprot.org/citations/28705318http://purl.uniprot.org/core/author"Ishikawa T."xsd:string
http://purl.uniprot.org/citations/28705318http://purl.uniprot.org/core/author"Kimoto H."xsd:string
http://purl.uniprot.org/citations/28705318http://purl.uniprot.org/core/author"Saito K."xsd:string
http://purl.uniprot.org/citations/28705318http://purl.uniprot.org/core/author"Ohno S."xsd:string
http://purl.uniprot.org/citations/28705318http://purl.uniprot.org/core/author"Shibata Y."xsd:string
http://purl.uniprot.org/citations/28705318http://purl.uniprot.org/core/author"Sasaki R."xsd:string
http://purl.uniprot.org/citations/28705318http://purl.uniprot.org/core/author"Mercier S."xsd:string
http://purl.uniprot.org/citations/28705318http://purl.uniprot.org/core/author"Nishii K."xsd:string
http://purl.uniprot.org/citations/28705318http://purl.uniprot.org/core/author"Le Marec H."xsd:string
http://purl.uniprot.org/citations/28705318http://purl.uniprot.org/core/author"Seki A."xsd:string
http://purl.uniprot.org/citations/28705318http://purl.uniprot.org/core/author"Redon R."xsd:string
http://purl.uniprot.org/citations/28705318http://purl.uniprot.org/core/author"Makita N."xsd:string
http://purl.uniprot.org/citations/28705318http://purl.uniprot.org/core/author"Barc J."xsd:string
http://purl.uniprot.org/citations/28705318http://purl.uniprot.org/core/author"Thollet A."xsd:string
http://purl.uniprot.org/citations/28705318http://purl.uniprot.org/core/author"Parent P."xsd:string
http://purl.uniprot.org/citations/28705318http://purl.uniprot.org/core/author"Mishima H."xsd:string
http://purl.uniprot.org/citations/28705318http://purl.uniprot.org/core/author"Yoshiura K.I."xsd:string
http://purl.uniprot.org/citations/28705318http://purl.uniprot.org/core/author"Hagiwara N."xsd:string
http://purl.uniprot.org/citations/28705318http://purl.uniprot.org/core/author"Schott J.J."xsd:string
http://purl.uniprot.org/citations/28705318http://purl.uniprot.org/core/author"Probst V."xsd:string
http://purl.uniprot.org/citations/28705318http://purl.uniprot.org/core/author"Urano M."xsd:string
▶