| http://purl.uniprot.org/citations/28743807 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/28743807 | http://www.w3.org/2000/01/rdf-schema#comment | "In Caenorhabditis elegans, Notch signaling regulates developmentally timed sleep during the transition from L4 larval stage to adulthood (L4/A) . To identify core sleep pathways and to find genes acting downstream of Notch signaling, we undertook the first genome-wide, classical genetic screen focused on C. elegans developmentally timed sleep. To increase screen efficiency, we first looked for mutations that suppressed inappropriate anachronistic sleep in adult hsp::osm-11 animals overexpressing the Notch coligand OSM-11 after heat shock. We retained suppressor lines that also had defects in L4/A developmentally timed sleep, without heat shock overexpression of the Notch coligand. Sixteen suppressor lines with defects in developmentally timed sleep were identified. One line carried a new allele of goa-1; loss of GOA-1 Gαo decreased C. elegans sleep. Another line carried a new allele of gpb-2, encoding a Gβ5 protein; Gβ5 proteins have not been previously implicated in sleep. In other scenarios, Gβ5 GPB-2 acts with regulators of G protein signaling (RGS proteins) EAT-16 and EGL-10 to terminate either EGL-30 Gαq signaling or GOA-1 Gαo signaling, respectively. We found that loss of Gβ5 GPB-2 or RGS EAT-16 decreased L4/A sleep. By contrast, EGL-10 loss had no impact. Instead, loss of RGS-1 and RGS-2 increased sleep. Combined, our results suggest that, in the context of L4/A sleep, GPB-2 predominantly acts with EAT-16 RGS to inhibit EGL-30 Gαq signaling. These results confirm the importance of G protein signaling in sleep and demonstrate that these core sleep pathways function genetically downstream of the Notch signaling events promoting sleep."xsd:string |
| http://purl.uniprot.org/citations/28743807 | http://purl.org/dc/terms/identifier | "doi:10.1534/g3.117.300071"xsd:string |
| http://purl.uniprot.org/citations/28743807 | http://purl.uniprot.org/core/author | "Huang H."xsd:string |
| http://purl.uniprot.org/citations/28743807 | http://purl.uniprot.org/core/author | "Hart A.C."xsd:string |
| http://purl.uniprot.org/citations/28743807 | http://purl.uniprot.org/core/author | "Zhu C.T."xsd:string |
| http://purl.uniprot.org/citations/28743807 | http://purl.uniprot.org/core/author | "Hayden D.J."xsd:string |
| http://purl.uniprot.org/citations/28743807 | http://purl.uniprot.org/core/author | "Skuja L.L."xsd:string |
| http://purl.uniprot.org/citations/28743807 | http://purl.uniprot.org/core/date | "2017"xsd:gYear |
| http://purl.uniprot.org/citations/28743807 | http://purl.uniprot.org/core/name | "G3 (Bethesda)"xsd:string |
| http://purl.uniprot.org/citations/28743807 | http://purl.uniprot.org/core/pages | "2907-2917"xsd:string |
| http://purl.uniprot.org/citations/28743807 | http://purl.uniprot.org/core/title | "Genome-Wide Screen for Genes Involved in Caenorhabditis elegans Developmentally Timed Sleep."xsd:string |
| http://purl.uniprot.org/citations/28743807 | http://purl.uniprot.org/core/volume | "7"xsd:string |
| http://purl.uniprot.org/citations/28743807 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/28743807 |
| http://purl.uniprot.org/citations/28743807 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/28743807 |
| http://purl.uniprot.org/uniprot/#_P51875-mappedCitation-28743807 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28743807 |
| http://purl.uniprot.org/uniprot/#_P18948-mappedCitation-28743807 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28743807 |
| http://purl.uniprot.org/uniprot/#_Q20636-mappedCitation-28743807 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28743807 |
| http://purl.uniprot.org/uniprot/P18948 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/28743807 |
| http://purl.uniprot.org/uniprot/P51875 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/28743807 |
| http://purl.uniprot.org/uniprot/Q20636 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/28743807 |