| http://purl.uniprot.org/citations/28770820 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/28770820 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/28770820 | http://www.w3.org/2000/01/rdf-schema#comment | "Botulinum neurotoxins are known to have seven serotypes (BoNT/A-G). Here we report a new BoNT serotype, tentatively named BoNT/X, which has the lowest sequence identity with other BoNTs and is not recognized by antisera against known BoNTs. Similar to BoNT/B/D/F/G, BoNT/X cleaves vesicle-associated membrane proteins (VAMP) 1, 2 and 3, but at a novel site (Arg66-Ala67 in VAMP2). Remarkably, BoNT/X is the only toxin that also cleaves non-canonical substrates VAMP4, VAMP5 and Ykt6. To validate its activity, a small amount of full-length BoNT/X was assembled by linking two non-toxic fragments using a transpeptidase (sortase). Assembled BoNT/X cleaves VAMP2 and VAMP4 in cultured neurons and causes flaccid paralysis in mice. Thus, BoNT/X is a novel BoNT with a unique substrate profile. Its discovery posts a challenge to develop effective countermeasures, provides a novel tool for studying intracellular membrane trafficking, and presents a new potential therapeutic toxin for modulating secretions in cells."xsd:string |
| http://purl.uniprot.org/citations/28770820 | http://purl.org/dc/terms/identifier | "doi:10.1038/ncomms14130"xsd:string |
| http://purl.uniprot.org/citations/28770820 | http://purl.org/dc/terms/identifier | "doi:10.1038/ncomms14130"xsd:string |
| http://purl.uniprot.org/citations/28770820 | http://purl.uniprot.org/core/author | "Dong M."xsd:string |
| http://purl.uniprot.org/citations/28770820 | http://purl.uniprot.org/core/author | "Dong M."xsd:string |
| http://purl.uniprot.org/citations/28770820 | http://purl.uniprot.org/core/author | "Masuyer G."xsd:string |
| http://purl.uniprot.org/citations/28770820 | http://purl.uniprot.org/core/author | "Masuyer G."xsd:string |
| http://purl.uniprot.org/citations/28770820 | http://purl.uniprot.org/core/author | "Shen Y."xsd:string |
| http://purl.uniprot.org/citations/28770820 | http://purl.uniprot.org/core/author | "Shen Y."xsd:string |
| http://purl.uniprot.org/citations/28770820 | http://purl.uniprot.org/core/author | "Zhang J."xsd:string |
| http://purl.uniprot.org/citations/28770820 | http://purl.uniprot.org/core/author | "Zhang J."xsd:string |
| http://purl.uniprot.org/citations/28770820 | http://purl.uniprot.org/core/author | "Zhang S."xsd:string |
| http://purl.uniprot.org/citations/28770820 | http://purl.uniprot.org/core/author | "Zhang S."xsd:string |
| http://purl.uniprot.org/citations/28770820 | http://purl.uniprot.org/core/author | "Stenmark P."xsd:string |
| http://purl.uniprot.org/citations/28770820 | http://purl.uniprot.org/core/author | "Stenmark P."xsd:string |
| http://purl.uniprot.org/citations/28770820 | http://purl.uniprot.org/core/author | "Henriksson L."xsd:string |
| http://purl.uniprot.org/citations/28770820 | http://purl.uniprot.org/core/author | "Henriksson L."xsd:string |
| http://purl.uniprot.org/citations/28770820 | http://purl.uniprot.org/core/author | "Lundin D."xsd:string |
| http://purl.uniprot.org/citations/28770820 | http://purl.uniprot.org/core/author | "Lundin D."xsd:string |
| http://purl.uniprot.org/citations/28770820 | http://purl.uniprot.org/core/author | "Martinez-Carranza M."xsd:string |
| http://purl.uniprot.org/citations/28770820 | http://purl.uniprot.org/core/author | "Martinez-Carranza M."xsd:string |
| http://purl.uniprot.org/citations/28770820 | http://purl.uniprot.org/core/author | "Miyashita S.I."xsd:string |
| http://purl.uniprot.org/citations/28770820 | http://purl.uniprot.org/core/author | "Miyashita S.I."xsd:string |