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http://purl.uniprot.org/citations/28800626http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/28800626http://www.w3.org/2000/01/rdf-schema#comment"

Background

Abdominal surgery and disease cause persistent abdominal adhesions, pelvic pain, infertility and occasionally, bowel obstruction. Current treatments are ineffective and the aetiology is unclear, although excessive collagen deposition is a consistent feature. Lysyl oxidase (Lox) is a key enzyme required for crosslinking and deposition of insoluble collagen, so we investigated whether targeting Lox might be an approach to reduce abdominal adhesions.

Methods

Female C57Bl/6 mice were treated intraperitoneally with multiwalled carbon nanotubes (NT) to induce fibrosis, together with chemical (ß-aminoproprionitrile-BAPN) or miRNA Lox inhibitors, progesterone or dexamethasone. Fibrotic lesions on the diaphragm, and expression of fibrosis-related genes in abdominal wall peritoneal mesothelial cells (PMC) were measured. Effects of BAPN and dexamethasone on collagen fibre alignment were observed by TEM. Isolated PMC were cultured with interleukin-1 alpha (IL-1α) and progesterone to determine effects on Lox mRNA in vitro.

Results

NT-induced fibrosis and collagen deposition on the diaphragm was ameliorated by BAPN, Lox miRNA, or steroids. BAPN and dexamethasone disrupted collagen fibres. NT increased PMC Lox, Col1a1, Col3a1 and Bmp1 mRNA, which was inhibited by steroids. Progesterone significantly inhibited IL-1α induced Lox expression by PMC in vitro.

Conclusion

Our results provide proof-of-concept that targeting peritoneal Lox could be an effective approach in ameliorating fibrosis and adhesion development."xsd:string
http://purl.uniprot.org/citations/28800626http://purl.org/dc/terms/identifier"doi:10.1371/journal.pone.0183013"xsd:string
http://purl.uniprot.org/citations/28800626http://purl.uniprot.org/core/author"Lu Y."xsd:string
http://purl.uniprot.org/citations/28800626http://purl.uniprot.org/core/author"Mason J.I."xsd:string
http://purl.uniprot.org/citations/28800626http://purl.uniprot.org/core/author"Wu X."xsd:string
http://purl.uniprot.org/citations/28800626http://purl.uniprot.org/core/author"Green R."xsd:string
http://purl.uniprot.org/citations/28800626http://purl.uniprot.org/core/author"Kadler K.E."xsd:string
http://purl.uniprot.org/citations/28800626http://purl.uniprot.org/core/author"Brown P."xsd:string
http://purl.uniprot.org/citations/28800626http://purl.uniprot.org/core/author"Critchley H.O.D."xsd:string
http://purl.uniprot.org/citations/28800626http://purl.uniprot.org/core/author"Poland C."xsd:string
http://purl.uniprot.org/citations/28800626http://purl.uniprot.org/core/author"Hillier S.G."xsd:string
http://purl.uniprot.org/citations/28800626http://purl.uniprot.org/core/author"Sarvi S."xsd:string
http://purl.uniprot.org/citations/28800626http://purl.uniprot.org/core/author"Harlow C.R."xsd:string
http://purl.uniprot.org/citations/28800626http://purl.uniprot.org/core/author"Gardiner F."xsd:string
http://purl.uniprot.org/citations/28800626http://purl.uniprot.org/core/author"van Deemter M."xsd:string
http://purl.uniprot.org/citations/28800626http://purl.uniprot.org/core/date"2017"xsd:gYear
http://purl.uniprot.org/citations/28800626http://purl.uniprot.org/core/name"PLoS One"xsd:string
http://purl.uniprot.org/citations/28800626http://purl.uniprot.org/core/pages"e0183013"xsd:string
http://purl.uniprot.org/citations/28800626http://purl.uniprot.org/core/title"Targeting lysyl oxidase reduces peritoneal fibrosis."xsd:string
http://purl.uniprot.org/citations/28800626http://purl.uniprot.org/core/volume"12"xsd:string
http://purl.uniprot.org/citations/28800626http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/28800626
http://purl.uniprot.org/citations/28800626http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/28800626
http://purl.uniprot.org/uniprot/P28301#attribution-7BA83A2E3D4E9CC2D53B6898E33A9797http://purl.uniprot.org/core/sourcehttp://purl.uniprot.org/citations/28800626
http://purl.uniprot.org/uniprot/#_Q3UXV2-mappedCitation-28800626http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/28800626
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