| http://purl.uniprot.org/citations/28808804 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/28808804 | http://www.w3.org/2000/01/rdf-schema#comment | "GM3, a major lipid component of the plasma membrane outer leaflet in mammalian cells, is synthesized in the luminal side of Golgi by ST3GAL5 protein (ST3G5), a type II membrane protein. Two strains of St3Gal5 knockout mice have been established for studies of GM3 physiological function: St3Gal5-Ex5-KO (lacking exon 5, which contains the catalytic domain of ST3G5), and St3Gal5-Ex3-KO (lacking exon 3, which contains the initiation codons). Results of the present study demonstrate that GM3 synthesis is still present, at a low level, in liver of St3Gal5-Ex3-KO mice. St3Gal5 has two mRNA transcriptional variants: a-type and b-type. When exon 3 is deleted, ST3G5 is not translated from a-type or b-type, as a result of initiation codon deletion or frame shift. Through NCBI database search and real-time PCR analyses of various mouse tissues, we identified a liver-specific St3Gal5 transcriptional variant (c-type) capable of producing artificial ST3G5 (M*-ST3G5) having GM3 synthase activity in the absence of exon 3. St3Gal5-Ex3-KO mice expressed c-type mRNA without exon 3 (c-type-/-) in liver. The transmembrane and catalytic domains of M*-ST3G5 translated from c-type-/- were identical to those from wild-type, although the cytoplasmic regions differed. Expression of M*-ST3G5 in embryonic fibroblasts derived from St3Gal5-Ex3-KO mice led to GM3 synthesis; M*-ST3G5 thus displayed enzyme activity in vivo. Taken together, our findings indicate that expression of liver-specific c-type variant accounts for the residual GM3 synthase activity observed in liver of St3Gal5-Ex3-KO mice."xsd:string |
| http://purl.uniprot.org/citations/28808804 | http://purl.org/dc/terms/identifier | "doi:10.1007/s10719-017-9788-1"xsd:string |
| http://purl.uniprot.org/citations/28808804 | http://purl.uniprot.org/core/author | "Nitta T."xsd:string |
| http://purl.uniprot.org/citations/28808804 | http://purl.uniprot.org/core/author | "Uemura S."xsd:string |
| http://purl.uniprot.org/citations/28808804 | http://purl.uniprot.org/core/author | "Shishido F."xsd:string |
| http://purl.uniprot.org/citations/28808804 | http://purl.uniprot.org/core/author | "Inokuchi J.I."xsd:string |
| http://purl.uniprot.org/citations/28808804 | http://purl.uniprot.org/core/date | "2017"xsd:gYear |
| http://purl.uniprot.org/citations/28808804 | http://purl.uniprot.org/core/name | "Glycoconj J"xsd:string |
| http://purl.uniprot.org/citations/28808804 | http://purl.uniprot.org/core/pages | "651-659"xsd:string |
| http://purl.uniprot.org/citations/28808804 | http://purl.uniprot.org/core/title | "Identification of a new liver-specific c-type mRNA transcriptional variant for mouse ST3GAL5 (GM3/GM4 synthase)."xsd:string |
| http://purl.uniprot.org/citations/28808804 | http://purl.uniprot.org/core/volume | "34"xsd:string |
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