| http://purl.uniprot.org/citations/28827366 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/28827366 | http://www.w3.org/2000/01/rdf-schema#comment | "The pathogenesis of Listeria monocytogenes depends on the ability of this bacterium to escape from the phagosome of the host cells via the action of the pore-forming toxin listeriolysin O (LLO). Expression of the LLO-encoding gene (hly) requires the transcriptional activator PrfA, and both hly and prfA genes are essential for L. monocytogenes virulence. Here, we used the hemolytic activity of LLO as a phenotypic marker to screen for spontaneous virulence-attenuating mutations in L. monocytogenes Sixty nonhemolytic isolates were identified among a collection of 57,820 confirmed L. monocytogenes strains isolated from a variety of sources (0.1%). In most cases (56/60; 93.3%), the nonhemolytic phenotype resulted from nonsense, missense, or frameshift mutations in prfA Five strains carried hly mutations leading to a single amino acid substitution (G299V) or a premature stop codon causing strong virulence attenuation in mice. In one strain, both hly and gshF (encoding a glutathione synthase required for full PrfA activity) were missing due to genomic rearrangements likely caused by a transposable element. The PrfA/LLO loss-of-function (PrfA-/LLO-) mutants belonged to phylogenetically diverse clades of L. monocytogenes, and most were identified among nonclinical strains (57/60). Consistent with the rare occurrence of loss-of-virulence mutations, we show that prfA and hly are under purifying selection. Although occurring at a low frequency, PrfA-/LLO- mutational events in L. monocytogenes lead to niche restriction and open an evolutionary path for obligate saprophytism in this facultative intracellular pathogen."xsd:string |
| http://purl.uniprot.org/citations/28827366 | http://purl.org/dc/terms/identifier | "doi:10.1128/iai.00541-17"xsd:string |
| http://purl.uniprot.org/citations/28827366 | http://purl.uniprot.org/core/author | "Chenal-Francisque V."xsd:string |
| http://purl.uniprot.org/citations/28827366 | http://purl.uniprot.org/core/author | "Han L."xsd:string |
| http://purl.uniprot.org/citations/28827366 | http://purl.uniprot.org/core/author | "Gouin E."xsd:string |
| http://purl.uniprot.org/citations/28827366 | http://purl.uniprot.org/core/author | "Lecuit M."xsd:string |
| http://purl.uniprot.org/citations/28827366 | http://purl.uniprot.org/core/author | "Vazquez-Boland J.A."xsd:string |
| http://purl.uniprot.org/citations/28827366 | http://purl.uniprot.org/core/author | "Moura A."xsd:string |
| http://purl.uniprot.org/citations/28827366 | http://purl.uniprot.org/core/author | "Scortti M."xsd:string |
| http://purl.uniprot.org/citations/28827366 | http://purl.uniprot.org/core/author | "Leclercq A."xsd:string |
| http://purl.uniprot.org/citations/28827366 | http://purl.uniprot.org/core/author | "Disson O."xsd:string |
| http://purl.uniprot.org/citations/28827366 | http://purl.uniprot.org/core/author | "Vales G."xsd:string |
| http://purl.uniprot.org/citations/28827366 | http://purl.uniprot.org/core/author | "Bracq-Dieye H."xsd:string |
| http://purl.uniprot.org/citations/28827366 | http://purl.uniprot.org/core/author | "Maury M.M."xsd:string |
| http://purl.uniprot.org/citations/28827366 | http://purl.uniprot.org/core/date | "2017"xsd:gYear |
| http://purl.uniprot.org/citations/28827366 | http://purl.uniprot.org/core/name | "Infect Immun"xsd:string |
| http://purl.uniprot.org/citations/28827366 | http://purl.uniprot.org/core/pages | "e00541-17"xsd:string |
| http://purl.uniprot.org/citations/28827366 | http://purl.uniprot.org/core/title | "Spontaneous Loss of Virulence in Natural Populations of Listeria monocytogenes."xsd:string |
| http://purl.uniprot.org/citations/28827366 | http://purl.uniprot.org/core/volume | "85"xsd:string |
| http://purl.uniprot.org/citations/28827366 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/28827366 |
| http://purl.uniprot.org/citations/28827366 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/28827366 |
| http://purl.uniprot.org/uniprot/#_P13128-mappedCitation-28827366 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28827366 |
| http://purl.uniprot.org/uniprot/#_P22262-mappedCitation-28827366 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28827366 |
| http://purl.uniprot.org/uniprot/P22262 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/28827366 |