| http://purl.uniprot.org/citations/2887570 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/2887570 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/2887570 | http://www.w3.org/2000/01/rdf-schema#comment | "2-(4-Bromo-2,3-dioxobutylthio)-1,N(6)-ethenoadenosine 2',5'-bisphosphate (2-BDB-T epsilon A-2',5'-DP) is an affinity label for the coenzyme-binding site of pig heart NADP+-dependent isocitrate dehydrogenase. Specific reaction occurs at the coenzyme site with an incorporation of 0.5 mol of reagent/mol of enzyme subunit (i.e. modification of only one subunit of the dimeric enzyme) (Bailey, J.M., and Colman, R.F. (1985) Biochemistry 24, 5367-5377). Modified enzyme, prepared by incubating 1 mg/ml isocitrate dehydrogenase with 75 microM 2-BDB-T epsilon A-2',5'-DP in the absence and presence of substrate or coenzyme, was reduced with NaBH4, carboxymethylated, and digested with trypsin. Nucleotidyl peptides were isolated by chromatography on DEAE-cellulose, followed by treatment with acid phosphatase (to decrease the negative charge by removing the phosphate groups from covalently bound reagent) and rechromatography on the same DEAE-cellulose column. The isolated peptides were characterized by amino acid analysis, dansylation, and gas-phase sequencing. A single triskaidekapeptide corresponding to modification of the coenzyme site by 2-BDB-T epsilon A-2',5'-DP was identified as: Asp-Leu-Ala-Gly-X-Ile-His-Gly-Leu-Ser-Asn-Val-Lys. Additional evidence indicated that X is a glutamate residue derivatized by 2-BDB-T epsilon A-2',5'-DP."xsd:string |
| http://purl.uniprot.org/citations/2887570 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0021-9258(18)45251-9"xsd:string |
| http://purl.uniprot.org/citations/2887570 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0021-9258(18)45251-9"xsd:string |
| http://purl.uniprot.org/citations/2887570 | http://purl.uniprot.org/core/author | "Colman R.F."xsd:string |
| http://purl.uniprot.org/citations/2887570 | http://purl.uniprot.org/core/author | "Colman R.F."xsd:string |
| http://purl.uniprot.org/citations/2887570 | http://purl.uniprot.org/core/author | "Bailey J.M."xsd:string |
| http://purl.uniprot.org/citations/2887570 | http://purl.uniprot.org/core/author | "Bailey J.M."xsd:string |
| http://purl.uniprot.org/citations/2887570 | http://purl.uniprot.org/core/date | "1987"xsd:gYear |
| http://purl.uniprot.org/citations/2887570 | http://purl.uniprot.org/core/date | "1987"xsd:gYear |
| http://purl.uniprot.org/citations/2887570 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/2887570 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/2887570 | http://purl.uniprot.org/core/pages | "12620-12626"xsd:string |
| http://purl.uniprot.org/citations/2887570 | http://purl.uniprot.org/core/pages | "12620-12626"xsd:string |
| http://purl.uniprot.org/citations/2887570 | http://purl.uniprot.org/core/title | "Isolation of the glutamyl peptide labeled by the nucleotide analogue 2-(4-bromo-2,3-dioxobutylthio)-1,N(6)-ethenoadenosine 2',5'-biphosphate in the active site of NADP+-specific isocitrate dehydrogenase."xsd:string |
| http://purl.uniprot.org/citations/2887570 | http://purl.uniprot.org/core/title | "Isolation of the glutamyl peptide labeled by the nucleotide analogue 2-(4-bromo-2,3-dioxobutylthio)-1,N(6)-ethenoadenosine 2',5'-biphosphate in the active site of NADP+-specific isocitrate dehydrogenase."xsd:string |
| http://purl.uniprot.org/citations/2887570 | http://purl.uniprot.org/core/volume | "262"xsd:string |
| http://purl.uniprot.org/citations/2887570 | http://purl.uniprot.org/core/volume | "262"xsd:string |
| http://purl.uniprot.org/citations/2887570 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/2887570 |
| http://purl.uniprot.org/citations/2887570 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/2887570 |
| http://purl.uniprot.org/citations/2887570 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/2887570 |
| http://purl.uniprot.org/citations/2887570 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/2887570 |
| http://purl.uniprot.org/uniprot/P20304 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/2887570 |
| http://purl.uniprot.org/uniprot/#_P20304-citation-2887570 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/2887570 |