Results

RDF/XMLNTriplesTurtleShow queryShare ▶
SubjectPredicateObject
http://purl.uniprot.org/citations/28906292http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/28906292http://www.w3.org/2000/01/rdf-schema#comment"

Background

The integrity of the gut barrier in patients with inflammatory bowel disease is known to be impaired but the exact mechanisms remain mostly unknown. SHANK3 mutations are associated with autism, and patients with autism are known to have higher proportions of inflammatory bowel disease. Here, we explore the role of SHANK3 in inflammatory bowel disease, both in vivo and in vitro.

Methods

Dextran sulfate sodium colitis was induced in SHANK3 knockout mice. Transepithelial electrical resistance, paracellular permeability, and Salmonella invasion assays were used to evaluate epithelial barrier function, in vitro and in vivo. Expression of tight junction proteins, protein kinases, and MAP kinase phosphorylation changes were analyzed by immunoblotting after overexpression or knockdown of SHANK3 expression. SHANK3 expression in intestinal tissue from patients with Crohn's disease was analyzed by quantitative polymerase chain reaction and immunohistochemistry.

Results

SHANK3 knockout mice were more susceptible to dextran sulfate sodium. SHANK3 knockout resulted in a leaky epithelial barrier phenotype, as demonstrated by decreased transepithelial electrical resistance, increased paracellular permeability, and increased Salmonella invasion. Overexpression of SHANK3 enhanced ZO-1 expression, and knockdown of SHANK3 resulted in decreased expression of ZO-1. Regulation of ZO-1 expression by SHANK3 seems to be mediated through a PKCε-dependent pathway. SHANK3 expression correlated with ZO-1 and PKCε in colonic tissue of patients with Crohn's disease.

Conclusions

The expression level of SHANK3 affects ZO-1 expression and the barrier function in intestinal epithelial cells. This may provide novel insights in Crohn's disease pathogenesis and treatment."xsd:string
http://purl.uniprot.org/citations/28906292http://purl.org/dc/terms/identifier"doi:10.1097/mib.0000000000001250"xsd:string
http://purl.uniprot.org/citations/28906292http://purl.uniprot.org/core/author"Xavier R.J."xsd:string
http://purl.uniprot.org/citations/28906292http://purl.uniprot.org/core/author"Hsiao J.H."xsd:string
http://purl.uniprot.org/citations/28906292http://purl.uniprot.org/core/author"Wong J.M."xsd:string
http://purl.uniprot.org/citations/28906292http://purl.uniprot.org/core/author"Lai L.C."xsd:string
http://purl.uniprot.org/citations/28906292http://purl.uniprot.org/core/author"Podolsky D.K."xsd:string
http://purl.uniprot.org/citations/28906292http://purl.uniprot.org/core/author"Tung C.C."xsd:string
http://purl.uniprot.org/citations/28906292http://purl.uniprot.org/core/author"Wei S.C."xsd:string
http://purl.uniprot.org/citations/28906292http://purl.uniprot.org/core/author"Yang-Yen H.F."xsd:string
http://purl.uniprot.org/citations/28906292http://purl.uniprot.org/core/author"Shun C.T."xsd:string
http://purl.uniprot.org/citations/28906292http://purl.uniprot.org/core/author"Ni Y.H."xsd:string
http://purl.uniprot.org/citations/28906292http://purl.uniprot.org/core/author"Chuang E.Y."xsd:string
http://purl.uniprot.org/citations/28906292http://purl.uniprot.org/core/author"Tsao P.N."xsd:string
http://purl.uniprot.org/citations/28906292http://purl.uniprot.org/core/author"Weng M.T."xsd:string
http://purl.uniprot.org/citations/28906292http://purl.uniprot.org/core/author"Yen J.J.Y."xsd:string
http://purl.uniprot.org/citations/28906292http://purl.uniprot.org/core/author"Yu L.C.H."xsd:string
http://purl.uniprot.org/citations/28906292http://purl.uniprot.org/core/date"2017"xsd:gYear
http://purl.uniprot.org/citations/28906292http://purl.uniprot.org/core/name"Inflamm Bowel Dis"xsd:string
http://purl.uniprot.org/citations/28906292http://purl.uniprot.org/core/pages"1730-1740"xsd:string
http://purl.uniprot.org/citations/28906292http://purl.uniprot.org/core/title"SHANK3 Regulates Intestinal Barrier Function Through Modulating ZO-1 Expression Through the PKCepsilon-dependent Pathway."xsd:string
http://purl.uniprot.org/citations/28906292http://purl.uniprot.org/core/volume"23"xsd:string
http://purl.uniprot.org/citations/28906292http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/28906292
http://purl.uniprot.org/citations/28906292http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/28906292
▶