| http://purl.uniprot.org/citations/28963472 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/28963472 | http://www.w3.org/2000/01/rdf-schema#comment | "The Cys2/His2-type zinc finger protein Zfp296 has been implicated in stem cell pluripotency and tumor pathogenesis. However, its mechanisms remain elusive. Here, we demonstrated that a Zfp296 deficiency in mice impairs germ-cell development and embryonic growth. Zfp296 was intracellularly localized to heterochromatin in embryos. A GST-Zfp296 pull-down experiment using ES cell nuclear extract followed by LC-MS/MS showed that Zfp296 interacts with component proteins of heterochromatin (such as HP1, Dnmt1, Dnmt3b, and ATRX) and the NuRD complex. We focused on H3K9 methylation as a hallmark of heterochromatin, and found that Zfp296 overexpression in cultured cells reduces the Suv39h1-mediated H3K9 methylation. Consistent with this finding, in Zfp296 -/- mouse embryos, we observed a global increase in H3K9 methylation in a developmental stage-dependent manner, and showed, by ChIP-qPCR, that the H3K9me3 levels at major satellite repeats were elevated in Zfp296 -/- embryos. Our results demonstrate that Zfp296 is a component of heterochromatin that affects embryonic development by negatively regulating H3K9 methylation."xsd:string |
| http://purl.uniprot.org/citations/28963472 | http://purl.org/dc/terms/identifier | "doi:10.1038/s41598-017-12772-y"xsd:string |
| http://purl.uniprot.org/citations/28963472 | http://purl.uniprot.org/core/author | "Miyazaki T."xsd:string |
| http://purl.uniprot.org/citations/28963472 | http://purl.uniprot.org/core/author | "Tashiro F."xsd:string |
| http://purl.uniprot.org/citations/28963472 | http://purl.uniprot.org/core/author | "Matsuura T."xsd:string |
| http://purl.uniprot.org/citations/28963472 | http://purl.uniprot.org/core/author | "Miyazaki S."xsd:string |
| http://purl.uniprot.org/citations/28963472 | http://purl.uniprot.org/core/author | "Miyazaki J.I."xsd:string |
| http://purl.uniprot.org/citations/28963472 | http://purl.uniprot.org/core/date | "2017"xsd:gYear |
| http://purl.uniprot.org/citations/28963472 | http://purl.uniprot.org/core/name | "Sci Rep"xsd:string |
| http://purl.uniprot.org/citations/28963472 | http://purl.uniprot.org/core/pages | "12462"xsd:string |
| http://purl.uniprot.org/citations/28963472 | http://purl.uniprot.org/core/title | "Zfp296 negatively regulates H3K9 methylation in embryonic development as a component of heterochromatin."xsd:string |
| http://purl.uniprot.org/citations/28963472 | http://purl.uniprot.org/core/volume | "7"xsd:string |
| http://purl.uniprot.org/citations/28963472 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/28963472 |
| http://purl.uniprot.org/citations/28963472 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/28963472 |
| http://purl.uniprot.org/uniprot/#_A0A0A6YWA0-mappedCitation-28963472 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28963472 |
| http://purl.uniprot.org/uniprot/#_A0A0A6YWE3-mappedCitation-28963472 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28963472 |
| http://purl.uniprot.org/uniprot/#_A0A0A6YXA8-mappedCitation-28963472 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28963472 |
| http://purl.uniprot.org/uniprot/#_A0A0A6YXD1-mappedCitation-28963472 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28963472 |
| http://purl.uniprot.org/uniprot/#_A0A0U1RNV6-mappedCitation-28963472 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28963472 |
| http://purl.uniprot.org/uniprot/#_A0A0U1RPI3-mappedCitation-28963472 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28963472 |
| http://purl.uniprot.org/uniprot/#_A0A023ULC4-mappedCitation-28963472 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28963472 |
| http://purl.uniprot.org/uniprot/#_A0A0G2JEQ4-mappedCitation-28963472 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28963472 |
| http://purl.uniprot.org/uniprot/#_A0A1B0GXE2-mappedCitation-28963472 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28963472 |
| http://purl.uniprot.org/uniprot/#_D3YYC3-mappedCitation-28963472 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/28963472 |