| http://purl.uniprot.org/citations/28965604 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/28965604 | http://www.w3.org/2000/01/rdf-schema#comment | "One consequence of lipopolysaccharide (LPS)-induced stimulation of macrophages is the release of Interferon (IFN)-β, and subsequently the activation of the JAK-STAT1 pathway, resulting in the expression of inducible nitric oxide synthase (iNOS). Free intracellular zinc ions (Zn2+) have a profound impact as a second messenger in LPS-dependent gene expression. Previous work had indicated a Zn2+-dependent upregulation of STAT1 mRNA in response to LPS and IFN-β, potentially affecting STAT1-dependent downstream signaling upon pre-incubation with these agents. The aim of the present study was to investigate the long-term influence of Zn2+ chelation on cellular STAT1 levels and their effect on protein levels and activity of iNOS. The LPS- and IFN-β-mediated increase of STAT1 mRNA and protein levels was abrogated by chelation of Zn2+ with the membrane permeable chelator N,N,N',N'-Tetrakis(2-pyridylmethyl)ethylenediamine (TPEN) in RAW 264.7 macrophages. After 48h pre-incubation together with IFN-β, TPEN also led to reduced nitric monoxide formation in response to a second stimulation with LPS. Nonetheless, the latter was observed regardless of any pre-incubation with IFN-β, suggesting that the effect of treatment with TPEN negatively affects iNOS induction independently from cellular STAT1 levels. In conclusion, long term Zn2+ chelation does affect STAT1 protein expression, but interferes with NO production by a different, yet unknown pathway not involving STAT1. However, as there are many additional STAT1-dependent genes, there might still be effects on targets other than iNOS."xsd:string |
| http://purl.uniprot.org/citations/28965604 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.jtemb.2017.05.011"xsd:string |
| http://purl.uniprot.org/citations/28965604 | http://purl.uniprot.org/core/author | "Brieger A."xsd:string |
| http://purl.uniprot.org/citations/28965604 | http://purl.uniprot.org/core/author | "Haase H."xsd:string |
| http://purl.uniprot.org/citations/28965604 | http://purl.uniprot.org/core/author | "Rink L."xsd:string |
| http://purl.uniprot.org/citations/28965604 | http://purl.uniprot.org/core/author | "Engelhardt G."xsd:string |
| http://purl.uniprot.org/citations/28965604 | http://purl.uniprot.org/core/author | "Hebel S."xsd:string |
| http://purl.uniprot.org/citations/28965604 | http://purl.uniprot.org/core/author | "Reiber C."xsd:string |
| http://purl.uniprot.org/citations/28965604 | http://purl.uniprot.org/core/date | "2017"xsd:gYear |
| http://purl.uniprot.org/citations/28965604 | http://purl.uniprot.org/core/name | "J Trace Elem Med Biol"xsd:string |
| http://purl.uniprot.org/citations/28965604 | http://purl.uniprot.org/core/pages | "76-82"xsd:string |
| http://purl.uniprot.org/citations/28965604 | http://purl.uniprot.org/core/title | "Zinc chelation decreases IFN-beta-induced STAT1 upregulation and iNOS expression in RAW 264.7 macrophages."xsd:string |
| http://purl.uniprot.org/citations/28965604 | http://purl.uniprot.org/core/volume | "44"xsd:string |
| http://purl.uniprot.org/citations/28965604 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/28965604 |
| http://purl.uniprot.org/citations/28965604 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/28965604 |
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