| http://purl.uniprot.org/citations/28965824 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/28965824 | http://www.w3.org/2000/01/rdf-schema#comment | "Theobromine, a methylxanthine derived from cacao beans, reportedly has various health-promoting properties but molecular mechanism by which effects of theobromine on adipocyte differentiation and adipogenesis remains unclear. In this study, we aimed to clarify the molecular mechanisms of the anti-adipogenic effect of theobromine in vitro and in vivo. ICR mice (4week-old) were administered with theobromine (0.1g/kg) for 7days. Theobromine administration attenuated gains in body and epididymal adipose tissue weights in mice and suppressed expression of adipogenic-associated genes in mouse adipose tissue. In 3T3-L1 preadipocytes, theobromine caused degradation of C/EBPβ protein by the ubiquitin-proteasome pathway. Pull down assay showed that theobromine selectively interacts with adenosine receptor A1 (AR1), and AR1 knockdown inhibited theobromine-induced C/EBPβ degradation. Theobromine increased sumoylation of C/EBPβ at Lys133. Expression of the small ubiquitin-like modifier (SUMO)-specific protease 2 (SENP2) gene, coding for a desumoylation enzyme, was suppressed by theobromine. In vivo knockdown studies showed that AR1 knockdown in mice attenuated the anti-adipogenic effects of theobromine in younger mice. Theobromine suppresses adipocyte differentiation and induced C/EBPβ degradation by increasing its sumoylation. Furthermore, the inhibition of AR1 signaling is important for theobromine-induced C/EBPβ degradation."xsd:string |
| http://purl.uniprot.org/citations/28965824 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bbamcr.2017.09.017"xsd:string |
| http://purl.uniprot.org/citations/28965824 | http://purl.uniprot.org/core/author | "Katayama S."xsd:string |
| http://purl.uniprot.org/citations/28965824 | http://purl.uniprot.org/core/author | "Nakamura S."xsd:string |
| http://purl.uniprot.org/citations/28965824 | http://purl.uniprot.org/core/author | "Watanabe S."xsd:string |
| http://purl.uniprot.org/citations/28965824 | http://purl.uniprot.org/core/author | "Yoshioka Y."xsd:string |
| http://purl.uniprot.org/citations/28965824 | http://purl.uniprot.org/core/author | "Ashida H."xsd:string |
| http://purl.uniprot.org/citations/28965824 | http://purl.uniprot.org/core/author | "Mitani T."xsd:string |
| http://purl.uniprot.org/citations/28965824 | http://purl.uniprot.org/core/date | "2017"xsd:gYear |
| http://purl.uniprot.org/citations/28965824 | http://purl.uniprot.org/core/name | "Biochim Biophys Acta Mol Cell Res"xsd:string |
| http://purl.uniprot.org/citations/28965824 | http://purl.uniprot.org/core/pages | "2438-2448"xsd:string |
| http://purl.uniprot.org/citations/28965824 | http://purl.uniprot.org/core/title | "Theobromine suppresses adipogenesis through enhancement of CCAAT-enhancer-binding protein beta degradation by adenosine receptor A1."xsd:string |
| http://purl.uniprot.org/citations/28965824 | http://purl.uniprot.org/core/volume | "1864"xsd:string |
| http://purl.uniprot.org/citations/28965824 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/28965824 |
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