| http://purl.uniprot.org/citations/28980861 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/28980861 | http://www.w3.org/2000/01/rdf-schema#comment | "Shugoshin is an evolutionarily conserved protein, which is involved in tension sensing on mitotic chromosomes, kinetochore biorientation, and protection of centromeric (CEN) cohesin for faithful chromosome segregation. Interaction of the C-terminus of Sgo1 with phosphorylated histone H2A regulates its association with CEN and pericentromeric (peri-CEN) chromatin, whereas mutations in histone H3 selectively compromise the association of Sgo1 with peri-CEN but not CEN chromatin. Given that histone H3 is absent from CEN and is replaced by a histone H3 variant CENP-ACse4, we investigated if CENP-ACse4 interacts with Sgo1 and promotes its association with the CEN chromatin. In this study, we found that Sgo1 interacts with CENP-ACse4 in vivo and in vitro. The N-terminus coiled-coil domain of Sgo1 without the C-terminus (sgo1-NT) is sufficient for its interaction with CENP-ACse4, association with CEN but not the peri-CEN, and this CEN association is cell cycle dependent with maximum enrichment in mitosis. In agreement with the role of CENP-ACse4 in CEN maintenance of Sgo1, depletion of CENP-ACse4 results in the loss of Sgo1 and sgo1-NT from the CEN chromatin. The N-terminus of Sgo1 is required for genome stability as a mutant lacking the N-terminus (sgo1-CT) exhibits increased chromosome missegregation when compared to a sgo1-NT mutant. In summary, our results define a novel role for the N-terminus of Sgo1 in CENP-ACse4 mediated recruitment of Sgo1 to CEN chromatin for faithful chromosome segregation."xsd:string |
| http://purl.uniprot.org/citations/28980861 | http://purl.org/dc/terms/identifier | "doi:10.1080/15384101.2017.1380129"xsd:string |
| http://purl.uniprot.org/citations/28980861 | http://purl.uniprot.org/core/author | "Chen P."xsd:string |
| http://purl.uniprot.org/citations/28980861 | http://purl.uniprot.org/core/author | "Wang S."xsd:string |
| http://purl.uniprot.org/citations/28980861 | http://purl.uniprot.org/core/author | "Hazbun T.R."xsd:string |
| http://purl.uniprot.org/citations/28980861 | http://purl.uniprot.org/core/author | "Mishra P.K."xsd:string |
| http://purl.uniprot.org/citations/28980861 | http://purl.uniprot.org/core/author | "Basrai M.A."xsd:string |
| http://purl.uniprot.org/citations/28980861 | http://purl.uniprot.org/core/author | "Thapa K.S."xsd:string |
| http://purl.uniprot.org/citations/28980861 | http://purl.uniprot.org/core/date | "2018"xsd:gYear |
| http://purl.uniprot.org/citations/28980861 | http://purl.uniprot.org/core/name | "Cell Cycle"xsd:string |
| http://purl.uniprot.org/citations/28980861 | http://purl.uniprot.org/core/pages | "11-23"xsd:string |
| http://purl.uniprot.org/citations/28980861 | http://purl.uniprot.org/core/title | "Budding yeast CENP-ACse4 interacts with the N-terminus of Sgo1 and regulates its association with centromeric chromatin."xsd:string |
| http://purl.uniprot.org/citations/28980861 | http://purl.uniprot.org/core/volume | "17"xsd:string |
| http://purl.uniprot.org/citations/28980861 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/28980861 |
| http://purl.uniprot.org/citations/28980861 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/28980861 |
| http://purl.uniprot.org/uniprot/P36012#attribution-830B25A082F001869D42970FF913AC86 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/28980861 |
| http://purl.uniprot.org/uniprot/Q08490#attribution-37A1BD9F34E0AF09CF9E648C4836B4FC | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/28980861 |