| http://purl.uniprot.org/citations/29035787 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/29035787 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/29035787 | http://www.w3.org/2000/01/rdf-schema#comment | "Spike (S) glycoprotein on the viral envelope is the main determinant of infectivity. The S protein of coronavirus infectious bronchitis virus (IBV) contains 29 putative asparagine(N)-linked glycosylation sites. These post-translational modifications may assist in protein folding and play important roles in the functionality of S protein. In this study, we used bioinformatics tools to predict N-linked glycosylation sites and to analyze their distribution in IBV strains and variants. Among these sites, 8 sites were confirmed in the S protein extracted from partially purified virus particles by proteomics approaches. N-D and N-Q substitutions at 13 predicted sites were introduced into an infectious clone system. The impact on S protein-mediated cell-cell fusion, viral recovery and infectivity was assessed, leading to the identification of sites essential for the functions of IBV S protein. Further characterization of these and other uncharacterized sites may reveal novel aspects of N-linked glycosylation in coronavirus replication and pathogenesis."xsd:string |
| http://purl.uniprot.org/citations/29035787 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.virol.2017.10.003"xsd:string |
| http://purl.uniprot.org/citations/29035787 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.virol.2017.10.003"xsd:string |
| http://purl.uniprot.org/citations/29035787 | http://purl.uniprot.org/core/author | "Liu D.X."xsd:string |
| http://purl.uniprot.org/citations/29035787 | http://purl.uniprot.org/core/author | "Liu D.X."xsd:string |
| http://purl.uniprot.org/citations/29035787 | http://purl.uniprot.org/core/author | "Yamada Y."xsd:string |
| http://purl.uniprot.org/citations/29035787 | http://purl.uniprot.org/core/author | "Yamada Y."xsd:string |
| http://purl.uniprot.org/citations/29035787 | http://purl.uniprot.org/core/author | "Zheng J."xsd:string |
| http://purl.uniprot.org/citations/29035787 | http://purl.uniprot.org/core/author | "Zheng J."xsd:string |
| http://purl.uniprot.org/citations/29035787 | http://purl.uniprot.org/core/author | "Huang M."xsd:string |
| http://purl.uniprot.org/citations/29035787 | http://purl.uniprot.org/core/author | "Huang M."xsd:string |
| http://purl.uniprot.org/citations/29035787 | http://purl.uniprot.org/core/author | "Fung T.S."xsd:string |
| http://purl.uniprot.org/citations/29035787 | http://purl.uniprot.org/core/author | "Fung T.S."xsd:string |
| http://purl.uniprot.org/citations/29035787 | http://purl.uniprot.org/core/author | "Chia R."xsd:string |
| http://purl.uniprot.org/citations/29035787 | http://purl.uniprot.org/core/author | "Chia R."xsd:string |
| http://purl.uniprot.org/citations/29035787 | http://purl.uniprot.org/core/date | "2018"xsd:gYear |
| http://purl.uniprot.org/citations/29035787 | http://purl.uniprot.org/core/date | "2018"xsd:gYear |
| http://purl.uniprot.org/citations/29035787 | http://purl.uniprot.org/core/name | "Virology"xsd:string |
| http://purl.uniprot.org/citations/29035787 | http://purl.uniprot.org/core/name | "Virology"xsd:string |
| http://purl.uniprot.org/citations/29035787 | http://purl.uniprot.org/core/pages | "65-74"xsd:string |
| http://purl.uniprot.org/citations/29035787 | http://purl.uniprot.org/core/pages | "65-74"xsd:string |
| http://purl.uniprot.org/citations/29035787 | http://purl.uniprot.org/core/title | "Identification of N-linked glycosylation sites in the spike protein and their functional impact on the replication and infectivity of coronavirus infectious bronchitis virus in cell culture."xsd:string |
| http://purl.uniprot.org/citations/29035787 | http://purl.uniprot.org/core/title | "Identification of N-linked glycosylation sites in the spike protein and their functional impact on the replication and infectivity of coronavirus infectious bronchitis virus in cell culture."xsd:string |