| http://purl.uniprot.org/citations/29077787 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/29077787 | http://www.w3.org/2000/01/rdf-schema#comment | "Although many miRNAs are reported to be involved in tumor formation and progression, the effect of miR-219a-5p on breast cancer metastasis is not well-known. The aim of this study is to investigate the effect of miR-219a-5p on the migratory ability and epithelial-mesenchymal transition (EMT) of breast cancer cells. First, miR-219a-5p was found to be highly expressed in low-invasive breast cancer MCF-7 cells, but lowly expressed in high-invasive breast cancer MDA-MB-231 cells. Wound scratch assay and transwell assay showed that miR-219a-5p inhibited the migratory ability of MDA-MB-231 cells. miR-219a-5p also suppressed the cellular EMT, confirmed by suppressing the expression of mesenchymal markers vimentin and N-cadherin and increasing the expression of epithelial marker E-cadherin. Using the epithelial-mesenchymal-epithelial model in MCF-7 cells, we confirmed that the level of miR-219a-5p was highly expressed in epithelial-type cells and lowly expressed in mesenchymal-type cells. Importantly, we identified myocardin-related transcription factor A (MRTF-A) as a novel potential target gene of miR-219a-5p. Overexpression of miR-219a-5p in MDA-MB-231 cells could inhibit the expression of MRTF-A as revealed by real-time PCR and western blot analysis. miR-219a-5p inhibited the transcription of MRTF-A by targeting the 3'UTR of MRTF-A, which was confirmed by wild-type or mutant MRTF-A 3'UTR luciferase reporter system. Furthermore, knockdown of MRTF-A using siRNA for MRTF-A could depress breast cell migration. In conclusion, our present study revealed the tumor suppressive role of miR-219a-5p in regulating breast cancer migration by targeting MRTF-A, suggesting that miR-219a-5p might be a therapeutic target in breast cancer through regulating EMT."xsd:string |
| http://purl.uniprot.org/citations/29077787 | http://purl.org/dc/terms/identifier | "doi:10.1093/abbs/gmx114"xsd:string |
| http://purl.uniprot.org/citations/29077787 | http://purl.uniprot.org/core/author | "Huang L."xsd:string |
| http://purl.uniprot.org/citations/29077787 | http://purl.uniprot.org/core/author | "He H."xsd:string |
| http://purl.uniprot.org/citations/29077787 | http://purl.uniprot.org/core/author | "Luo X."xsd:string |
| http://purl.uniprot.org/citations/29077787 | http://purl.uniprot.org/core/author | "Zhou H."xsd:string |
| http://purl.uniprot.org/citations/29077787 | http://purl.uniprot.org/core/author | "Zhang T."xsd:string |
| http://purl.uniprot.org/citations/29077787 | http://purl.uniprot.org/core/author | "Wang H."xsd:string |
| http://purl.uniprot.org/citations/29077787 | http://purl.uniprot.org/core/author | "Yuan Y."xsd:string |
| http://purl.uniprot.org/citations/29077787 | http://purl.uniprot.org/core/author | "Wang N."xsd:string |
| http://purl.uniprot.org/citations/29077787 | http://purl.uniprot.org/core/author | "Song T."xsd:string |
| http://purl.uniprot.org/citations/29077787 | http://purl.uniprot.org/core/author | "Huo L."xsd:string |
| http://purl.uniprot.org/citations/29077787 | http://purl.uniprot.org/core/author | "Zhuang C."xsd:string |
| http://purl.uniprot.org/citations/29077787 | http://purl.uniprot.org/core/date | "2017"xsd:gYear |
| http://purl.uniprot.org/citations/29077787 | http://purl.uniprot.org/core/name | "Acta Biochim Biophys Sin (Shanghai)"xsd:string |
| http://purl.uniprot.org/citations/29077787 | http://purl.uniprot.org/core/pages | "1112-1121"xsd:string |
| http://purl.uniprot.org/citations/29077787 | http://purl.uniprot.org/core/title | "miR-219a-5p inhibits breast cancer cell migration and epithelial-mesenchymal transition by targeting myocardin-related transcription factor A."xsd:string |
| http://purl.uniprot.org/citations/29077787 | http://purl.uniprot.org/core/volume | "49"xsd:string |
| http://purl.uniprot.org/citations/29077787 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/29077787 |
| http://purl.uniprot.org/citations/29077787 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/29077787 |
| http://purl.uniprot.org/uniprot/#_B0QY84-mappedCitation-29077787 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/29077787 |
| http://purl.uniprot.org/uniprot/#_A4FUJ8-mappedCitation-29077787 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/29077787 |
| http://purl.uniprot.org/uniprot/#_L8E8L9-mappedCitation-29077787 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/29077787 |
| http://purl.uniprot.org/uniprot/#_Q29R68-mappedCitation-29077787 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/29077787 |