Results

RDF/XMLNTriplesTurtleShow queryShare ▶
SubjectPredicateObject
http://purl.uniprot.org/citations/29118970http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/29118970http://www.w3.org/2000/01/rdf-schema#comment"

Background

The 14-3-3 family of proteins have been reported to play an important role in development in various mouse models, but the context specific developmental functions of 14-3-3ζ remain to be determined. In this study, we identified a context specific developmental function of 14-3-3ζ.

Results

Targeted deletion of 14-3-3ζ in the C57Bl/6J murine genetic background led to neonatal lethality due to respiratory distress and could be rescued by out-breeding to the CD-1 or backcrossing to the FVB/NJ congenic background. Histological analysis of lung sections from 18.5 days post coitum embryos (dpc) showed that 14-3-3ζ-/-lung development is arrested at the pseudoglandular stage and exhibits vascular defects. The expression of miR-126, an endothelial-specific miRNA known to regulate lung vascular integrity was down-regulated in the lungs of the 14-3-3ζ-/-embryos in the C57Bl/6J background as compared to their wild-type counterparts. Loss of 14-3-3ζ in endothelial cells inhibited the angiogenic capability of the endothelial cells as determined by both trans-well migration assays and tube formation assays and these defects could be rescued by re-expressing miR-126. Mechanistically, loss of 14-3-3ζ led to reduced Erk1/2 phosphorylation resulting in attenuated binding of the transcription factor Ets2 on the miR-126 promoter which ultimately reduced expression of miR-126.

Conclusion

Our data demonstrates that miR-126 is an important angiogenesis regulator that functions downstream of 14-3-3ζ and downregulation of miR-126 plays a critical role in 14-3-3ζ-loss induced defects in lung vasculature in the C57Bl/6J genetic background."xsd:string
http://purl.uniprot.org/citations/29118970http://purl.org/dc/terms/identifier"doi:10.1186/s13578-017-0186-y"xsd:string
http://purl.uniprot.org/citations/29118970http://purl.uniprot.org/core/author"Li P."xsd:string
http://purl.uniprot.org/citations/29118970http://purl.uniprot.org/core/author"Yang J."xsd:string
http://purl.uniprot.org/citations/29118970http://purl.uniprot.org/core/author"Xiao Y."xsd:string
http://purl.uniprot.org/citations/29118970http://purl.uniprot.org/core/author"Wang J."xsd:string
http://purl.uniprot.org/citations/29118970http://purl.uniprot.org/core/author"Wang Q."xsd:string
http://purl.uniprot.org/citations/29118970http://purl.uniprot.org/core/author"Wang H."xsd:string
http://purl.uniprot.org/citations/29118970http://purl.uniprot.org/core/author"Yu D."xsd:string
http://purl.uniprot.org/citations/29118970http://purl.uniprot.org/core/author"Xiong Y."xsd:string
http://purl.uniprot.org/citations/29118970http://purl.uniprot.org/core/author"Behringer R.R."xsd:string
http://purl.uniprot.org/citations/29118970http://purl.uniprot.org/core/author"Joshi S."xsd:string
http://purl.uniprot.org/citations/29118970http://purl.uniprot.org/core/author"Parker-Thornburg J."xsd:string
http://purl.uniprot.org/citations/29118970http://purl.uniprot.org/core/date"2017"xsd:gYear
http://purl.uniprot.org/citations/29118970http://purl.uniprot.org/core/name"Cell Biosci"xsd:string
http://purl.uniprot.org/citations/29118970http://purl.uniprot.org/core/pages"58"xsd:string
http://purl.uniprot.org/citations/29118970http://purl.uniprot.org/core/title"14-3-3zeta loss leads to neonatal lethality by microRNA-126 downregulation-mediated developmental defects in lung vasculature."xsd:string
http://purl.uniprot.org/citations/29118970http://purl.uniprot.org/core/volume"7"xsd:string
http://purl.uniprot.org/citations/29118970http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/29118970
http://purl.uniprot.org/citations/29118970http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/29118970
http://purl.uniprot.org/uniprot/P63101#attribution-0CB25646314265810AD6210A824272E1http://purl.uniprot.org/core/sourcehttp://purl.uniprot.org/citations/29118970
http://purl.uniprot.org/uniprot/#_A0A0A6YX84-mappedCitation-29118970http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/29118970
http://purl.uniprot.org/uniprot/#_A0A1B0GQV9-mappedCitation-29118970http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/29118970
http://purl.uniprot.org/uniprot/#_D3YW45-mappedCitation-29118970http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/29118970
▶