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http://purl.uniprot.org/citations/29124886http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/29124886http://www.w3.org/2000/01/rdf-schema#comment"

Aim

Lung cancer is typically categorized into small cell lung cancer (SCLC) and non-small cell lung cancer (NSCLC). NSCLC comprises of the majority of lung cancer with a poor prognosis in advanced cases. Transcriptional profiling studies, including microarrays and RNA-sequencing studies, have significantly enriched our knowledge of gene expression patterns in NSCLC. A recent transcriptional profiling study identified high prevalence of CBX3/HP1-gamma upregulation in human NSCLC samples. CBX3/HP1-gamma is an isoform of the heterochromatin protein 1 family, which plays a role in heterochromatin formation and is linked to cancer.

Methods

We examined lung cancer samples from our hospital using immunohistochemistry for CBX3/HP1-gamma staining. We also analyzed publicly available databases of NSCLC transcriptional profiling to validate our results.

Results

We identified a high prevalence (77.2%) of samples with positive CBX3/HP1-gamma staining by immunohistochemistry in NSCLC patient samples. Independently, we queried a publicly available dataset (GSE40419) containing RNA-seq data from 77 patients. Upregulation of CBX3/HP1-gamma in tumor samples was present in 60.2% of the patients. A similar correlation was also observed in the The Cancer Genome Atlas (TCGA) database. Interestingly, we discovered a highly significant association between positive CBX3/HP1-gamma staining and EGFR mutation in our patient samples (40 of 42 patients, P < 0.001). Treatment of EGFR mutant NSCLC cell lines with the EGFR inhibitor gefitinib failed to yield a change in CBX/HP1-gamma expression, suggesting that CBX/HP1-gamma expression may be independent of EGFR downstream signaling.

Conclusion

We report a significant upregulation of CBX3/HP1-gamma in NSCLC patients, and also a possible relationship between CBX3/HP1-gamma expression and EGFR mutation."xsd:string
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http://purl.uniprot.org/citations/29124886http://purl.uniprot.org/core/author"Chang S.C."xsd:string
http://purl.uniprot.org/citations/29124886http://purl.uniprot.org/core/author"Chen Y.C."xsd:string
http://purl.uniprot.org/citations/29124886http://purl.uniprot.org/core/author"Wang W.S."xsd:string
http://purl.uniprot.org/citations/29124886http://purl.uniprot.org/core/author"Lai Y.C."xsd:string
http://purl.uniprot.org/citations/29124886http://purl.uniprot.org/core/author"Wang N.K."xsd:string
http://purl.uniprot.org/citations/29124886http://purl.uniprot.org/core/author"Lai J.I."xsd:string
http://purl.uniprot.org/citations/29124886http://purl.uniprot.org/core/date"2018"xsd:gYear
http://purl.uniprot.org/citations/29124886http://purl.uniprot.org/core/name"Asia Pac J Clin Oncol"xsd:string
http://purl.uniprot.org/citations/29124886http://purl.uniprot.org/core/pages"e283-e288"xsd:string
http://purl.uniprot.org/citations/29124886http://purl.uniprot.org/core/title"CBX3/heterochromatin protein 1 gamma is significantly upregulated in patients with non-small cell lung cancer."xsd:string
http://purl.uniprot.org/citations/29124886http://purl.uniprot.org/core/volume"14"xsd:string
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