Results

RDF/XMLNTriplesTurtleShow queryShare ▶
SubjectPredicateObject
http://purl.uniprot.org/citations/29187452http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/29187452http://www.w3.org/2000/01/rdf-schema#comment"

Background/aim

Transforming growth factor β (TGFβ) plays a role in diverse oncogenic pathways including cell proliferation and cell motility and is regulated by the pleiotropic factor Y-box binding protein-1 (YB-1). In breast cancer, Sma/Mad related protein 2 (Smad2) represents the most common downstream transducer in TGFβ signaling.

Materials and methods

Here, YB-1's impact on Smad2 phospho-activation was characterized by incubation of the breast cancer cell line MCF-7 with or without TGFβ1 in the absence or presence of overexpressed YB-1 protein. The phospho-status of Smad2 was assessed via western blotting.

Results

Analysis of MCF-7 cells revealed no induction of total Smad2 neither in the presence of TGFβ1, nor during YB-1 overexpression. In contrast, incubation with TGFβ1 led to an increase of phosphorylated Smad2 forms which was significantly amplified by simultaneously overexpressed YB-1 (2.8±0.2-fold).

Conclusion

Oncogenic YB-1 indirectly enhances TGFβ signaling cascades via Smad2 phospho-activation and may represent a promising factor for future diagnosis and therapy of breast cancer."xsd:string
http://purl.uniprot.org/citations/29187452http://purl.org/dc/terms/identifier"doi:10.21873/anticanres.12134"xsd:string
http://purl.uniprot.org/citations/29187452http://purl.uniprot.org/core/author"Weiss M."xsd:string
http://purl.uniprot.org/citations/29187452http://purl.uniprot.org/core/author"Joffroy C."xsd:string
http://purl.uniprot.org/citations/29187452http://purl.uniprot.org/core/author"Stope M.B."xsd:string
http://purl.uniprot.org/citations/29187452http://purl.uniprot.org/core/author"Knabbe C."xsd:string
http://purl.uniprot.org/citations/29187452http://purl.uniprot.org/core/author"Mustea A."xsd:string
http://purl.uniprot.org/citations/29187452http://purl.uniprot.org/core/author"Koensgen D."xsd:string
http://purl.uniprot.org/citations/29187452http://purl.uniprot.org/core/author"Buck M.B."xsd:string
http://purl.uniprot.org/citations/29187452http://purl.uniprot.org/core/author"Popp S.L."xsd:string
http://purl.uniprot.org/citations/29187452http://purl.uniprot.org/core/date"2017"xsd:gYear
http://purl.uniprot.org/citations/29187452http://purl.uniprot.org/core/name"Anticancer Res"xsd:string
http://purl.uniprot.org/citations/29187452http://purl.uniprot.org/core/pages"6745-6748"xsd:string
http://purl.uniprot.org/citations/29187452http://purl.uniprot.org/core/title"Y-box Binding Protein-1 Enhances Oncogenic Transforming Growth Factor beta Signaling in Breast Cancer Cells pii>viapi/i> Triggering Phospho-Activation of Smad2."xsd:string
http://purl.uniprot.org/citations/29187452http://purl.uniprot.org/core/volume"37"xsd:string
http://purl.uniprot.org/citations/29187452http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/29187452
http://purl.uniprot.org/citations/29187452http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/29187452
http://purl.uniprot.org/uniprot/#_A0A078BBI5-mappedCitation-29187452http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/29187452
http://purl.uniprot.org/uniprot/#_A0A078BC11-mappedCitation-29187452http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/29187452
http://purl.uniprot.org/uniprot/#_A0A078BCJ0-mappedCitation-29187452http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/29187452
http://purl.uniprot.org/uniprot/#_A0A078BCH8-mappedCitation-29187452http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/29187452
http://purl.uniprot.org/uniprot/#_A0A078BFK3-mappedCitation-29187452http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/29187452
http://purl.uniprot.org/uniprot/#_A0A510GAF6-mappedCitation-29187452http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/29187452
http://purl.uniprot.org/uniprot/#_A0A499FJK2-mappedCitation-29187452http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/29187452
▶