| http://purl.uniprot.org/citations/29192197 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/29192197 | http://www.w3.org/2000/01/rdf-schema#comment | "Human SLURP-1 is a secreted protein of the Ly6/uPAR/three-finger neurotoxin family that co-localizes with nicotinic acetylcholine receptors (nAChRs) and modulates their functions. Conflicting biological activities of SLURP-1 at various nAChR subtypes have been based on heterologously produced SLURP-1 containing N-and/or C-terminal extensions. Here, we report the chemical synthesis of the 81 amino acid residue human SLURP-1 protein, characterization of its 3D structure by NMR, and its biological activity at nAChR subtypes. Radioligand assays indicated that synthetic SLURP-1 did not compete with [125I]-α-bungarotoxin (α-Bgt) binding to human neuronal α7 and Torpedo californica muscle-type nAChRs, nor to mollusk acetylcholine binding proteins (AChBP). Inhibition of human α7-mediated currents only occurred in the presence of the allosteric modulator PNU120596. In contrast, we observed robust SLURP-1 mediated inhibition of human α3β4, α4β4, α3β2 nAChRs, as well as human and rat α9α10 nAChRs. SLURP-1 inhibition of α9α10 nAChRs was accentuated at higher ACh concentrations, indicating an allosteric binding mechanism. Our results are discussed in the context of recent studies on heterologously produced SLURP-1 and indicate that N-terminal extensions of SLURP-1 may affect its activity and selectivity on its targets. In this respect, synthetic SLURP-1 appears to be a better probe for structure-function studies."xsd:string |
| http://purl.uniprot.org/citations/29192197 | http://purl.org/dc/terms/identifier | "doi:10.1038/s41598-017-16809-0"xsd:string |
| http://purl.uniprot.org/citations/29192197 | http://purl.uniprot.org/core/author | "Adams D.J."xsd:string |
| http://purl.uniprot.org/citations/29192197 | http://purl.uniprot.org/core/author | "Durek T."xsd:string |
| http://purl.uniprot.org/citations/29192197 | http://purl.uniprot.org/core/author | "Kasheverov I.E."xsd:string |
| http://purl.uniprot.org/citations/29192197 | http://purl.uniprot.org/core/author | "Kudryavtsev D.S."xsd:string |
| http://purl.uniprot.org/citations/29192197 | http://purl.uniprot.org/core/author | "Tae H.S."xsd:string |
| http://purl.uniprot.org/citations/29192197 | http://purl.uniprot.org/core/author | "Shelukhina I.V."xsd:string |
| http://purl.uniprot.org/citations/29192197 | http://purl.uniprot.org/core/author | "Tsetlin V.I."xsd:string |
| http://purl.uniprot.org/citations/29192197 | http://purl.uniprot.org/core/author | "Craik D.J."xsd:string |
| http://purl.uniprot.org/citations/29192197 | http://purl.uniprot.org/core/author | "Corringer P.J."xsd:string |
| http://purl.uniprot.org/citations/29192197 | http://purl.uniprot.org/core/author | "Faure G."xsd:string |
| http://purl.uniprot.org/citations/29192197 | http://purl.uniprot.org/core/author | "Thongyoo P."xsd:string |
| http://purl.uniprot.org/citations/29192197 | http://purl.uniprot.org/core/author | "Spirova E.N."xsd:string |
| http://purl.uniprot.org/citations/29192197 | http://purl.uniprot.org/core/date | "2017"xsd:gYear |
| http://purl.uniprot.org/citations/29192197 | http://purl.uniprot.org/core/name | "Sci Rep"xsd:string |
| http://purl.uniprot.org/citations/29192197 | http://purl.uniprot.org/core/pages | "16606"xsd:string |
| http://purl.uniprot.org/citations/29192197 | http://purl.uniprot.org/core/title | "Interaction of Synthetic Human SLURP-1 with the Nicotinic Acetylcholine Receptors."xsd:string |
| http://purl.uniprot.org/citations/29192197 | http://purl.uniprot.org/core/volume | "7"xsd:string |
| http://purl.uniprot.org/citations/29192197 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/29192197 |
| http://purl.uniprot.org/citations/29192197 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/29192197 |
| http://purl.uniprot.org/uniprot/#_P55000-mappedCitation-29192197 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/29192197 |
| http://purl.uniprot.org/uniprot/P55000 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/29192197 |