| http://purl.uniprot.org/citations/29217522 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/29217522 | http://www.w3.org/2000/01/rdf-schema#comment | "Syd-1 proteins are required for presynaptic development in worm, fly, and mouse. Syd-1 proteins in all three species contain a Rho GTPase activating protein (GAP)-like domain of unclear significance: invertebrate Syd-1s are thought to lack GAP activity, and mouse mSYD1A has GAP activity that is thought to be dispensable for its function. Here, we show that Drosophila melanogaster Syd-1 can interact with all six fly Rhos and has GAP activity toward Rac1 and Cdc42. During development, fly Syd-1 clusters multiple presynaptic proteins at the neuromuscular junction (NMJ), including the cell adhesion molecule Neurexin (Nrx-1) and the active zone (AZ) component Bruchpilot (Brp), both of which Syd-1 binds directly. We show that a mutant form of Syd-1 that specifically lacks GAP activity localizes normally to presynaptic sites and is sufficient to recruit Nrx-1 but fails to cluster Brp normally. We provide evidence that Syd-1 participates with Rac1 in two separate functions: (1) together with the Rac guanine exchange factor (RacGEF) Trio, GAP-active Syd-1 is required to regulate the nucleotide-bound state of Rac1, thereby promoting Brp clustering; and (2) Syd-1, independent of its GAP activity, is required for the recruitment of Nrx-1 to boutons, including the recruitment of Nrx-1 that is promoted by GTP-bound Rac1. We conclude that, contrary to current models, the GAP domain of fly Syd-1 is active and required for presynaptic development; we suggest that the same may be true of vertebrate Syd-1 proteins. In addition, our data provide new molecular insight into the ability of Rac1 to promote presynaptic development."xsd:string |
| http://purl.uniprot.org/citations/29217522 | http://purl.org/dc/terms/identifier | "doi:10.1534/genetics.117.300538"xsd:string |
| http://purl.uniprot.org/citations/29217522 | http://purl.uniprot.org/core/author | "Herman T.G."xsd:string |
| http://purl.uniprot.org/citations/29217522 | http://purl.uniprot.org/core/author | "Spinner M.A."xsd:string |
| http://purl.uniprot.org/citations/29217522 | http://purl.uniprot.org/core/author | "Walla D.A."xsd:string |
| http://purl.uniprot.org/citations/29217522 | http://purl.uniprot.org/core/date | "2018"xsd:gYear |
| http://purl.uniprot.org/citations/29217522 | http://purl.uniprot.org/core/name | "Genetics"xsd:string |
| http://purl.uniprot.org/citations/29217522 | http://purl.uniprot.org/core/pages | "705-716"xsd:string |
| http://purl.uniprot.org/citations/29217522 | http://purl.uniprot.org/core/title | "Drosophila Syd-1 Has RhoGAP Activity That Is Required for Presynaptic Clustering of Bruchpilot/ELKS but Not Neurexin-1."xsd:string |
| http://purl.uniprot.org/citations/29217522 | http://purl.uniprot.org/core/volume | "208"xsd:string |
| http://purl.uniprot.org/citations/29217522 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/29217522 |
| http://purl.uniprot.org/citations/29217522 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/29217522 |
| http://purl.uniprot.org/uniprot/#_A0A0B4KF38-mappedCitation-29217522 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/29217522 |
| http://purl.uniprot.org/uniprot/#_A0A0B4K6V0-mappedCitation-29217522 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/29217522 |
| http://purl.uniprot.org/uniprot/#_A0A0B4K6W5-mappedCitation-29217522 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/29217522 |
| http://purl.uniprot.org/uniprot/#_A0A0B4K7V8-mappedCitation-29217522 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/29217522 |
| http://purl.uniprot.org/uniprot/#_A0A0B4K813-mappedCitation-29217522 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/29217522 |
| http://purl.uniprot.org/uniprot/#_A0A0B4K843-mappedCitation-29217522 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/29217522 |
| http://purl.uniprot.org/uniprot/#_A0A0B4KH61-mappedCitation-29217522 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/29217522 |
| http://purl.uniprot.org/uniprot/#_A0A0B4LJ24-mappedCitation-29217522 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/29217522 |
| http://purl.uniprot.org/uniprot/#_A0A0B4K7G3-mappedCitation-29217522 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/29217522 |
| http://purl.uniprot.org/uniprot/#_A0A0B4K7K9-mappedCitation-29217522 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/29217522 |
| http://purl.uniprot.org/uniprot/#_A0A0B4K7S3-mappedCitation-29217522 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/29217522 |
| http://purl.uniprot.org/uniprot/#_A0A0B4KGL0-mappedCitation-29217522 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/29217522 |