| http://purl.uniprot.org/citations/29235485 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/29235485 | http://www.w3.org/2000/01/rdf-schema#comment | "This study aims to improve our understanding of the interaction between olfactory receptors and odorants to develop highly selective biosensing devices. Natural nanovesicles (NVs) from Saccharomyces cerevisiae, ~100 nm in diameter, carrying either the human OR17-40 or the chimpanzee OR7D4 olfactory receptor (OR) tagged with the c-myc epitope at their N-terminus, are presented as model systems to quantify the interaction between odorant and olfactory receptors. The level of expression of olfactory receptors was determined at individual NVs using a novel competitive ELISA immunoassay comparing the values obtained against those from techniques involving the solubilization of cell membrane proteins and the identification of c-myc-carrying receptors. Surface Plasmon Resonance (SPR) measurements on L1 Biacore chips indicate that cognate odorants bind to their Ors, thereby quantifying the approximate number of odorants that interact with a given olfactory receptor. The selectivity of OR17-40-carrying NVs towards helional and OR7D4-carrying NVs towards androstenone has been proven in cross-check experiments with non-specific odorant molecules (heptanal and pentadecalactone, respectively) and in control receptors."xsd:string |
| http://purl.uniprot.org/citations/29235485 | http://purl.org/dc/terms/identifier | "doi:10.1038/s41598-017-16997-9"xsd:string |
| http://purl.uniprot.org/citations/29235485 | http://purl.uniprot.org/core/author | "Marco M.P."xsd:string |
| http://purl.uniprot.org/citations/29235485 | http://purl.uniprot.org/core/author | "Taules M."xsd:string |
| http://purl.uniprot.org/citations/29235485 | http://purl.uniprot.org/core/author | "Calo A."xsd:string |
| http://purl.uniprot.org/citations/29235485 | http://purl.uniprot.org/core/author | "Samitier J."xsd:string |
| http://purl.uniprot.org/citations/29235485 | http://purl.uniprot.org/core/author | "Galve R."xsd:string |
| http://purl.uniprot.org/citations/29235485 | http://purl.uniprot.org/core/author | "Iavicoli P."xsd:string |
| http://purl.uniprot.org/citations/29235485 | http://purl.uniprot.org/core/author | "Sanmarti-Espinal M."xsd:string |
| http://purl.uniprot.org/citations/29235485 | http://purl.uniprot.org/core/date | "2017"xsd:gYear |
| http://purl.uniprot.org/citations/29235485 | http://purl.uniprot.org/core/name | "Sci Rep"xsd:string |
| http://purl.uniprot.org/citations/29235485 | http://purl.uniprot.org/core/pages | "17483"xsd:string |
| http://purl.uniprot.org/citations/29235485 | http://purl.uniprot.org/core/title | "Quantification of interacting cognate odorants with olfactory receptors in nanovesicles."xsd:string |
| http://purl.uniprot.org/citations/29235485 | http://purl.uniprot.org/core/volume | "7"xsd:string |
| http://purl.uniprot.org/citations/29235485 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/29235485 |
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