| http://purl.uniprot.org/citations/29269875 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/29269875 | http://www.w3.org/2000/01/rdf-schema#comment | "Eukaryotic origins of replication are licensed upon loading of the MCM helicase motor onto DNA. ATP hydrolysis by MCM is required for loading and the post-catalytic MCM is an inactive double hexamer that encircles duplex DNA. Origin firing depends on MCM engagement of Cdc45 and GINS to form the CMG holo-helicase. CMG assembly requires several steps including MCM phosphorylation by DDK. To understand origin activation, here we have determined the cryo-EM structures of DNA-bound MCM, either unmodified or phosphorylated, and visualize a phospho-dependent MCM element likely important for Cdc45 recruitment. MCM pore loops touch both the Watson and Crick strands, constraining duplex DNA in a bent configuration. By comparing our new MCM-DNA structure with the structure of CMG-DNA, we suggest how the conformational transition from the loaded, post-catalytic MCM to CMG might promote DNA untwisting and melting at the onset of replication."xsd:string |
| http://purl.uniprot.org/citations/29269875 | http://purl.org/dc/terms/identifier | "doi:10.1038/s41467-017-02389-0"xsd:string |
| http://purl.uniprot.org/citations/29269875 | http://purl.uniprot.org/core/author | "Costa A."xsd:string |
| http://purl.uniprot.org/citations/29269875 | http://purl.uniprot.org/core/author | "Diffley J.F.X."xsd:string |
| http://purl.uniprot.org/citations/29269875 | http://purl.uniprot.org/core/author | "Pye V.E."xsd:string |
| http://purl.uniprot.org/citations/29269875 | http://purl.uniprot.org/core/author | "Locke J."xsd:string |
| http://purl.uniprot.org/citations/29269875 | http://purl.uniprot.org/core/author | "Nans A."xsd:string |
| http://purl.uniprot.org/citations/29269875 | http://purl.uniprot.org/core/author | "Abid Ali F."xsd:string |
| http://purl.uniprot.org/citations/29269875 | http://purl.uniprot.org/core/author | "Douglas M.E."xsd:string |
| http://purl.uniprot.org/citations/29269875 | http://purl.uniprot.org/core/date | "2017"xsd:gYear |
| http://purl.uniprot.org/citations/29269875 | http://purl.uniprot.org/core/name | "Nat Commun"xsd:string |
| http://purl.uniprot.org/citations/29269875 | http://purl.uniprot.org/core/pages | "2241"xsd:string |
| http://purl.uniprot.org/citations/29269875 | http://purl.uniprot.org/core/title | "Cryo-EM structure of a licensed DNA replication origin."xsd:string |
| http://purl.uniprot.org/citations/29269875 | http://purl.uniprot.org/core/volume | "8"xsd:string |
| http://purl.uniprot.org/citations/29269875 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/29269875 |
| http://purl.uniprot.org/citations/29269875 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/29269875 |
| http://purl.uniprot.org/uniprot/#_P29469-mappedCitation-29269875 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/29269875 |
| http://purl.uniprot.org/uniprot/#_P29496-mappedCitation-29269875 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/29269875 |
| http://purl.uniprot.org/uniprot/#_P24279-mappedCitation-29269875 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/29269875 |
| http://purl.uniprot.org/uniprot/#_P38132-mappedCitation-29269875 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/29269875 |
| http://purl.uniprot.org/uniprot/#_P30665-mappedCitation-29269875 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/29269875 |
| http://purl.uniprot.org/uniprot/#_P53091-mappedCitation-29269875 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/29269875 |
| http://purl.uniprot.org/uniprot/P38132 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/29269875 |
| http://purl.uniprot.org/uniprot/P30665 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/29269875 |