Results

RDF/XMLNTriplesTurtleShow queryShare ▶
SubjectPredicateObject
http://purl.uniprot.org/citations/29316178http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/29316178http://www.w3.org/2000/01/rdf-schema#comment"

Background

Current flow-cytometric plasma cell (PC) gating is based on CD138, CD38, and CD45 expression. CD138 is known for variable expression and loss during storage and processing. Introduction of anti-CD38 and anti-CD138 monoclonal-antibody therapies has limited the use of these markers during follow-up. Hence, additional reliable PC-gating markers are required. Recently, CD229 has been claimed as an alternative PC-gating marker. However, these studies are limited to a small cohort of samples. We evaluated the utility of CD229 as a new PC-gating marker in routine laboratory practice.

Methods

Expression of CD229 was studied in 310 bone marrow (BM) samples (251 plasma-cell disorders and 59 controls) and compared with CD138 and CD38 expression. We also evaluated the effect of additional processing for cytoplasmic immunoglobulin-light-chains (CyIgL) staining on the quantitation of PC.

Results

Expression of CD229 was consistently stronger on PC than other hematopoietic-cells (p < 0.001). PC-percentages using CD229 in combination with CD38 or CD138 and CD45 revealed high correlation with a reference gating-strategy using CD138, CD38 and CD45 (r = 0.98, r = 0.99 r = 0.99 respectively) and r = 0.92 using CD229 and CD45 without CD38 or CD138. In contrast, CD138 expression showed significant variability (CV-MFI, 97.5) and loss from PC in 53% of samples. Quantitation of PC was found to be lower in 69.3% and higher in 30.7% samples processed for CyIgL-staining as compared to surface-staining.

Conclusions

CD229 is a reliable new alternative PC-gating marker in routine laboratory practice. Quantitation of PC based on CD138 expression or from samples processed for CyIgL-staining should be used with caution. © 2018 International Clinical Cytometry Society."xsd:string
http://purl.uniprot.org/citations/29316178http://purl.org/dc/terms/identifier"doi:10.1002/cyto.b.21619"xsd:string
http://purl.uniprot.org/citations/29316178http://purl.uniprot.org/core/author"Deshpande N."xsd:string
http://purl.uniprot.org/citations/29316178http://purl.uniprot.org/core/author"Chatterjee G."xsd:string
http://purl.uniprot.org/citations/29316178http://purl.uniprot.org/core/author"Cherian K."xsd:string
http://purl.uniprot.org/citations/29316178http://purl.uniprot.org/core/author"Badrinath Y."xsd:string
http://purl.uniprot.org/citations/29316178http://purl.uniprot.org/core/author"Gujral S."xsd:string
http://purl.uniprot.org/citations/29316178http://purl.uniprot.org/core/author"Subramanian P.G."xsd:string
http://purl.uniprot.org/citations/29316178http://purl.uniprot.org/core/author"Ghogale S."xsd:string
http://purl.uniprot.org/citations/29316178http://purl.uniprot.org/core/author"Patkar N.V."xsd:string
http://purl.uniprot.org/citations/29316178http://purl.uniprot.org/core/author"Tembhare P.R."xsd:string
http://purl.uniprot.org/citations/29316178http://purl.uniprot.org/core/author"Kedia S."xsd:string
http://purl.uniprot.org/citations/29316178http://purl.uniprot.org/core/author"Tauro W."xsd:string
http://purl.uniprot.org/citations/29316178http://purl.uniprot.org/core/date"2018"xsd:gYear
http://purl.uniprot.org/citations/29316178http://purl.uniprot.org/core/name"Cytometry B Clin Cytom"xsd:string
http://purl.uniprot.org/citations/29316178http://purl.uniprot.org/core/pages"509-519"xsd:string
http://purl.uniprot.org/citations/29316178http://purl.uniprot.org/core/title"Evaluation of CD229 as a new alternative plasma cell gating marker in the flow cytometric immunophenotyping of monoclonal gammopathies."xsd:string
http://purl.uniprot.org/citations/29316178http://purl.uniprot.org/core/volume"94"xsd:string
http://purl.uniprot.org/citations/29316178http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/29316178
http://purl.uniprot.org/citations/29316178http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/29316178
http://purl.uniprot.org/uniprot/#_A0A0C4DFU4-mappedCitation-29316178http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/29316178
http://purl.uniprot.org/uniprot/#_B4E0J5-mappedCitation-29316178http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/29316178
http://purl.uniprot.org/uniprot/#_Q05CA2-mappedCitation-29316178http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/29316178
http://purl.uniprot.org/uniprot/#_Q5VYH9-mappedCitation-29316178http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/29316178
▶