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Background

Acute myeloid leukemia (AML) with mutated nucleophosmin (NPM1) has been recognized as a distinct leukemia entity in the 2016 World Health Organization (WHO) classification. The genetic events underlying oncogenesis in NPM1-mutated AML that is characterized by a normal karyotype remain unclear. Inositol polyphosphate 4-phosphatase type II (INPP4B), a new factor in the phosphoinositide-3 kinase (PI3K) pathway-associated cancers, has been recently found a clinically relevant role in AML. However, little is known about the specific mechanistic function of INPP4B in NPM1-mutated AML.

Methods

The INPP4B expression levels in NPM1-mutated AML primary blasts and AML OCI-AML3 cell lines were determined by qRT-PCR and western blotting. The effect of INPP4B knockdown on OCI-AML3 leukemia cell proliferation was evaluated, using the Cell Counting Kit-8 and colony formation assay. After INPP4B overexpression or knockdown, the activation of serum and glucocorticoid-regulated kinase 3 (SGK3) and AKT was assessed. The effects of PI3K signaling pathway inhibitors on the levels of p-SGK3 in OCI-AML3 cells were tested. The mass of PI (3,4) P2 and PI (3) P was analyzed by ELISA upon INPP4B overexpression. Knockdown of SGK3 by RNA interference and a rescue assay were performed to confirm the critical role of SGK3 in INPP4B-mediated cell survival. In addition, the molecular mechanism underlying INPP4B expression in NPM1-mutated leukemia cells was explored. Finally, Kaplan-Meier survival analysis was conducted on the NPM1-mutated AML cohort stratified into quartiles for INPP4B expression in The Cancer Genome Atlas (TCGA) dataset.

Results

High expression of INPP4B was observed in NPM1-mutated AML. Knockdown of INPP4B repressed cell proliferation in OCI-AML3 cells, whereas recovered INPP4B rescued this inhibitory effect in vitro. Mechanically, INPP4B enhanced phosphorylated SGK3 (p-SGK3) status, but did not affect AKT activation. SGK3 was required for INPP4B-induced cell proliferation in OCI-AML3 cells. High levels of INPP4B were at least partially caused by the NPM1 mutant via ERK/Ets-1 signaling. Finally, high expression of INPP4B showed a trend towards lower overall survival and event-free survival in NPM1-mutated AML patients.

Conclusions

Our results indicate that INPP4B promotes leukemia cell survival via SGK3 activation, and INPP4B might be a potential target in the treatment of NPM1-mutated AML."xsd:string
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http://purl.uniprot.org/citations/29343273http://purl.uniprot.org/core/author"Tang Y."xsd:string
http://purl.uniprot.org/citations/29343273http://purl.uniprot.org/core/author"Tao Y."xsd:string
http://purl.uniprot.org/citations/29343273http://purl.uniprot.org/core/author"Zhang S."xsd:string
http://purl.uniprot.org/citations/29343273http://purl.uniprot.org/core/author"Wang L."xsd:string
http://purl.uniprot.org/citations/29343273http://purl.uniprot.org/core/author"Yang Z."xsd:string
http://purl.uniprot.org/citations/29343273http://purl.uniprot.org/core/author"Zhang L."xsd:string
http://purl.uniprot.org/citations/29343273http://purl.uniprot.org/core/author"Yang L."xsd:string
http://purl.uniprot.org/citations/29343273http://purl.uniprot.org/core/author"Zou Q."xsd:string
http://purl.uniprot.org/citations/29343273http://purl.uniprot.org/core/author"Jing Y."xsd:string
http://purl.uniprot.org/citations/29343273http://purl.uniprot.org/core/author"Zhan Q."xsd:string
http://purl.uniprot.org/citations/29343273http://purl.uniprot.org/core/author"Xian J."xsd:string
http://purl.uniprot.org/citations/29343273http://purl.uniprot.org/core/date"2018"xsd:gYear
http://purl.uniprot.org/citations/29343273http://purl.uniprot.org/core/name"J Exp Clin Cancer Res"xsd:string
http://purl.uniprot.org/citations/29343273http://purl.uniprot.org/core/pages"8"xsd:string
http://purl.uniprot.org/citations/29343273http://purl.uniprot.org/core/title"INPP4B promotes cell survival via SGK3 activation in NPM1-mutated leukemia."xsd:string
http://purl.uniprot.org/citations/29343273http://purl.uniprot.org/core/volume"37"xsd:string
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