| http://purl.uniprot.org/citations/29356380 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/29356380 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/29356380 | http://www.w3.org/2000/01/rdf-schema#comment | "The emergence and dissemination of multidrug resistant (MDR) pathogens resistant to nearly all available antibiotics poses a significant threat in clinical therapy. Among them, Klebsiella pneumoniae clinical isolates overexpressing KPC-2 carbapenemase are the most worrisome, extending bacterial resistance to last-resort carbapenems. In this study, we investigate the molecular recognition requirements in the KPC-2 active site by small phenylboronic acid derivatives. Four new phenylboronic acid derivatives were designed and tested against KPC-2. For the most active, despite their simple chemical structures, nanomolar affinity was achieved. The new derivatives restored susceptibility to meropenem in clinical strains overexpressing KPC-2. Moreover, no cytotoxicity was detected in cell-viability assays, which further validated the designed leads. Two crystallographic binary complexes of the best inhibitors binding KPC-2 were obtained at high resolution. Kinetic descriptions of slow binding, time-dependent inhibition, and interaction geometries in KPC-2 were fully investigated. This study will ultimately lead toward the optimization and development of more-effective KPC-2 inhibitors."xsd:string |
| http://purl.uniprot.org/citations/29356380 | http://purl.org/dc/terms/identifier | "doi:10.1002/cmdc.201700788"xsd:string |
| http://purl.uniprot.org/citations/29356380 | http://purl.org/dc/terms/identifier | "doi:10.1002/cmdc.201700788"xsd:string |
| http://purl.uniprot.org/citations/29356380 | http://purl.uniprot.org/core/author | "Cendron L."xsd:string |
| http://purl.uniprot.org/citations/29356380 | http://purl.uniprot.org/core/author | "Cendron L."xsd:string |
| http://purl.uniprot.org/citations/29356380 | http://purl.uniprot.org/core/author | "Perilli M."xsd:string |
| http://purl.uniprot.org/citations/29356380 | http://purl.uniprot.org/core/author | "Perilli M."xsd:string |
| http://purl.uniprot.org/citations/29356380 | http://purl.uniprot.org/core/author | "Blazquez J."xsd:string |
| http://purl.uniprot.org/citations/29356380 | http://purl.uniprot.org/core/author | "Blazquez J."xsd:string |
| http://purl.uniprot.org/citations/29356380 | http://purl.uniprot.org/core/author | "Oliver A."xsd:string |
| http://purl.uniprot.org/citations/29356380 | http://purl.uniprot.org/core/author | "Oliver A."xsd:string |
| http://purl.uniprot.org/citations/29356380 | http://purl.uniprot.org/core/author | "Celenza G."xsd:string |
| http://purl.uniprot.org/citations/29356380 | http://purl.uniprot.org/core/author | "Celenza G."xsd:string |
| http://purl.uniprot.org/citations/29356380 | http://purl.uniprot.org/core/author | "Bellio P."xsd:string |
| http://purl.uniprot.org/citations/29356380 | http://purl.uniprot.org/core/author | "Bellio P."xsd:string |
| http://purl.uniprot.org/citations/29356380 | http://purl.uniprot.org/core/author | "Linciano P."xsd:string |
| http://purl.uniprot.org/citations/29356380 | http://purl.uniprot.org/core/author | "Linciano P."xsd:string |
| http://purl.uniprot.org/citations/29356380 | http://purl.uniprot.org/core/author | "Tondi D."xsd:string |
| http://purl.uniprot.org/citations/29356380 | http://purl.uniprot.org/core/author | "Tondi D."xsd:string |
| http://purl.uniprot.org/citations/29356380 | http://purl.uniprot.org/core/author | "Vicario M."xsd:string |
| http://purl.uniprot.org/citations/29356380 | http://purl.uniprot.org/core/author | "Vicario M."xsd:string |
| http://purl.uniprot.org/citations/29356380 | http://purl.uniprot.org/core/date | "2018"xsd:gYear |
| http://purl.uniprot.org/citations/29356380 | http://purl.uniprot.org/core/date | "2018"xsd:gYear |