| http://purl.uniprot.org/citations/29361132 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/29361132 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/29361132 | http://www.w3.org/2000/01/rdf-schema#comment | "Poly(ADP-ribose) polymerases (PARPs) act as DNA break sensors and catalyze the synthesis of polymers of ADP-ribose (PAR) covalently attached to acceptor proteins at DNA damage sites. It has been demonstrated that both mammalian PARP1 and PARP2 PARylate double-strand break termini in DNA oligonucleotide duplexes in vitro. Here, we show that mammalian PARP2 and PARP3 can PARylate and mono(ADP-ribosyl)ate (MARylate), respectively, 5'- and 3'-terminal phosphate residues at double- and single-strand break termini of a DNA molecule containing multiple strand breaks. PARP3-catalyzed DNA MARylation can be considered a new type of reversible post-replicative DNA modification. According to DNA substrate specificity of PARP3 and PARP2, we propose a putative mechanistic model of PARP-catalyzed strand break-oriented ADP-ribosylation of DNA termini. Notably, PARP-mediated DNA ADP-ribosylation can be more effective than PARPs' auto-ADP-ribosylation depending on the DNA substrates and reaction conditions used. Finally, we show an effective PARP3- or PARP2-catalyzed ADP-ribosylation of high-molecular-weight (∼3-kb) DNA molecules, PARP-mediated DNA PARylation in cell-free extracts and a persisting signal of anti-PAR antibodies in a serially purified genomic DNA from bleomycin-treated poly(ADP-ribose) glycohydrolase-depleted HeLa cells. These results suggest that certain types of complex DNA breaks can be effectively ADP-ribosylated by PARPs in cellular response to DNA damage."xsd:string |
| http://purl.uniprot.org/citations/29361132 | http://purl.org/dc/terms/identifier | "doi:10.1093/nar/gkx1318"xsd:string |
| http://purl.uniprot.org/citations/29361132 | http://purl.org/dc/terms/identifier | "doi:10.1093/nar/gkx1318"xsd:string |
| http://purl.uniprot.org/citations/29361132 | http://purl.uniprot.org/core/author | "Gasparutto D."xsd:string |
| http://purl.uniprot.org/citations/29361132 | http://purl.uniprot.org/core/author | "Gasparutto D."xsd:string |
| http://purl.uniprot.org/citations/29361132 | http://purl.uniprot.org/core/author | "Saint-Pierre C."xsd:string |
| http://purl.uniprot.org/citations/29361132 | http://purl.uniprot.org/core/author | "Saint-Pierre C."xsd:string |
| http://purl.uniprot.org/citations/29361132 | http://purl.uniprot.org/core/author | "Talhaoui I."xsd:string |
| http://purl.uniprot.org/citations/29361132 | http://purl.uniprot.org/core/author | "Talhaoui I."xsd:string |
| http://purl.uniprot.org/citations/29361132 | http://purl.uniprot.org/core/author | "Lavrik O.I."xsd:string |
| http://purl.uniprot.org/citations/29361132 | http://purl.uniprot.org/core/author | "Lavrik O.I."xsd:string |
| http://purl.uniprot.org/citations/29361132 | http://purl.uniprot.org/core/author | "Kutuzov M.M."xsd:string |
| http://purl.uniprot.org/citations/29361132 | http://purl.uniprot.org/core/author | "Kutuzov M.M."xsd:string |
| http://purl.uniprot.org/citations/29361132 | http://purl.uniprot.org/core/author | "Biard D."xsd:string |
| http://purl.uniprot.org/citations/29361132 | http://purl.uniprot.org/core/author | "Biard D."xsd:string |
| http://purl.uniprot.org/citations/29361132 | http://purl.uniprot.org/core/author | "Ishchenko A.A."xsd:string |
| http://purl.uniprot.org/citations/29361132 | http://purl.uniprot.org/core/author | "Ishchenko A.A."xsd:string |
| http://purl.uniprot.org/citations/29361132 | http://purl.uniprot.org/core/author | "Matkarimov B.T."xsd:string |
| http://purl.uniprot.org/citations/29361132 | http://purl.uniprot.org/core/author | "Matkarimov B.T."xsd:string |
| http://purl.uniprot.org/citations/29361132 | http://purl.uniprot.org/core/author | "Zarkovic G."xsd:string |
| http://purl.uniprot.org/citations/29361132 | http://purl.uniprot.org/core/author | "Zarkovic G."xsd:string |
| http://purl.uniprot.org/citations/29361132 | http://purl.uniprot.org/core/author | "Belousova E.A."xsd:string |
| http://purl.uniprot.org/citations/29361132 | http://purl.uniprot.org/core/author | "Belousova E.A."xsd:string |