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http://purl.uniprot.org/citations/29367253http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/29367253http://www.w3.org/2000/01/rdf-schema#comment"Positive-strand RNA viruses, including picornaviruses, utilize cellular machinery for genome replication. Previously, we reported that each of the 2B, 2BC, 2C, 3A, and 3AB proteins of Aichi virus (AiV), a picornavirus, forms a complex with the Golgi apparatus protein ACBD3 and phosphatidylinositol 4-kinase IIIβ (PI4KB) at viral RNA replication sites (replication organelles [ROs]), enhancing PI4KB-dependent phosphatidylinositol 4-phosphate (PI4P) production. Here, we demonstrate AiV hijacking of the cellular cholesterol transport system involving oxysterol-binding protein (OSBP), a PI4P-binding cholesterol transfer protein. AiV RNA replication was inhibited by silencing cellular proteins known to be components of this pathway, OSBP, the ER membrane proteins VAPA and VAPB (VAP-A/B), the PI4P-phosphatase SAC1, and PI-transfer protein β. OSBP, VAP-A/B, and SAC1 were present at RNA replication sites. We also found various previously unknown interactions among the AiV proteins (2B, 2BC, 2C, 3A, and 3AB), ACBD3, OSBP, VAP-A/B, and SAC1, and the interactions were suggested to be involved in recruiting the component proteins to AiV ROs. Importantly, the OSBP-2B interaction enabled PI4P-independent recruitment of OSBP to AiV ROs, indicating preferential recruitment of OSBP among PI4P-binding proteins. Protein-protein interaction-based OSBP recruitment has not been reported for other picornaviruses. Cholesterol was accumulated at AiV ROs, and inhibition of OSBP-mediated cholesterol transfer impaired cholesterol accumulation and AiV RNA replication. Electron microscopy showed that AiV-induced vesicle-like structures were close to ER membranes. Altogether, we conclude that AiV directly recruits the cholesterol transport machinery through protein-protein interactions, resulting in formation of membrane contact sites between the ER and AiV ROs and cholesterol supply to the ROs.IMPORTANCE Positive-strand RNA viruses utilize host pathways to modulate the lipid composition of viral RNA replication sites for replication. Previously, we demonstrated that Aichi virus (AiV), a picornavirus, forms a complex comprising certain proteins of AiV, the Golgi apparatus protein ACBD3, and the lipid kinase PI4KB to synthesize PI4P lipid at the sites for AiV RNA replication. Here, we confirmed cholesterol accumulation at the AiV RNA replication sites, which are established by hijacking the host cholesterol transfer machinery mediated by a PI4P-binding cholesterol transfer protein, OSBP. We showed that the component proteins of the machinery, OSBP, VAP, SAC1, and PITPNB, are all essential host factors for AiV replication. Importantly, the machinery is directly recruited to the RNA replication sites through previously unknown interactions of VAP/OSBP/SAC1 with the AiV proteins and with ACBD3. Consequently, we propose a specific strategy employed by AiV to efficiently accumulate cholesterol at the RNA replication sites via protein-protein interactions."xsd:string
http://purl.uniprot.org/citations/29367253http://purl.org/dc/terms/identifier"doi:10.1128/jvi.01952-17"xsd:string
http://purl.uniprot.org/citations/29367253http://purl.uniprot.org/core/author"Nagashima S."xsd:string
http://purl.uniprot.org/citations/29367253http://purl.uniprot.org/core/author"Taniguchi K."xsd:string
http://purl.uniprot.org/citations/29367253http://purl.uniprot.org/core/author"Sasaki J."xsd:string
http://purl.uniprot.org/citations/29367253http://purl.uniprot.org/core/author"Ishikawa-Sasaki K."xsd:string
http://purl.uniprot.org/citations/29367253http://purl.uniprot.org/core/date"2018"xsd:gYear
http://purl.uniprot.org/citations/29367253http://purl.uniprot.org/core/name"J Virol"xsd:string
http://purl.uniprot.org/citations/29367253http://purl.uniprot.org/core/pages"e01952-17"xsd:string
http://purl.uniprot.org/citations/29367253http://purl.uniprot.org/core/title"Model of OSBP-Mediated Cholesterol Supply to Aichi Virus RNA Replication Sites Involving Protein-Protein Interactions among Viral Proteins, ACBD3, OSBP, VAP-A/B, and SAC1."xsd:string
http://purl.uniprot.org/citations/29367253http://purl.uniprot.org/core/volume"92"xsd:string
http://purl.uniprot.org/citations/29367253http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/29367253
http://purl.uniprot.org/citations/29367253http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/29367253
http://purl.uniprot.org/uniprot/#_E9PGZ4-mappedCitation-29367253http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/29367253
http://purl.uniprot.org/uniprot/#_A0A9L9PXK1-mappedCitation-29367253http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/29367253
http://purl.uniprot.org/uniprot/#_A8KA83-mappedCitation-29367253http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/29367253
http://purl.uniprot.org/uniprot/#_B4DVV3-mappedCitation-29367253http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/29367253
http://purl.uniprot.org/uniprot/#_B4DNS4-mappedCitation-29367253http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/29367253
http://purl.uniprot.org/uniprot/#_B3KVU7-mappedCitation-29367253http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/29367253
http://purl.uniprot.org/uniprot/#_B3KX17-mappedCitation-29367253http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/29367253
http://purl.uniprot.org/uniprot/#_B4DR25-mappedCitation-29367253http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/29367253
http://purl.uniprot.org/uniprot/#_Q53XM7-mappedCitation-29367253http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/29367253
http://purl.uniprot.org/uniprot/#_O95292-mappedCitation-29367253http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/29367253
http://purl.uniprot.org/uniprot/#_O43466-mappedCitation-29367253http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/29367253
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