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http://purl.uniprot.org/citations/29482609http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/29482609http://www.w3.org/2000/01/rdf-schema#comment"

Background

Interleukin-1β (IL-1β) is a pivotal proinflammatory cytokine that is strongly associated with the inflammation of gout. However, the underlying mechanism through which the production of IL-1β is regulated has not been fully elucidated. Our previous work identified that miR-302b had an important immune regulatory role in bacterial lung infections. This study was conducted to evaluate the function of miR-302b on monosodium urate (MSU) crystal-induced inflammation and its mechanism.

Methods

The expression pattern and the immune-regulatory role of miR-302b were evaluated both in vitro and in vivo. The functional targets of miR-302b were predicted by bioinformatics, and then validated by genetic approaches. In addition, the clinical feature of miR-302b was analyzed using serum samples of patients with gouty arthritis.

Results

The extremely high expression of miR-302b was observed in both macrophages and mouse air membranes treated with MSU. Intriguingly, overexpression of miR-302b regulated NF-κB and caspase-1 signaling, leading to significantly attenuate MSU-induced IL-1β. By genetic analysis, miR-302b exhibited inhibitory function on IRAK4 and EphA2 by binding to their 3'-UTR regions. Corporately silencing IRAK4 and EphA2 largely impaired MSU-induced IL-1β protein production. Moreover, it was also found that miR-302b and EphA2 suppressed the migration of macrophages. Finally, it was observed that high expression of miR-302b was a general feature in patients with gouty arthritis.

Conclusions

These results suggest that miR-302b can regulate IL-1β production in MSU-induced inflammation by targeting NF-κB and caspase-1 signaling, and may be a potential therapeutic target for gouty arthritis."xsd:string
http://purl.uniprot.org/citations/29482609http://purl.org/dc/terms/identifier"doi:10.1186/s13075-018-1528-9"xsd:string
http://purl.uniprot.org/citations/29482609http://purl.uniprot.org/core/author"Guo M."xsd:string
http://purl.uniprot.org/citations/29482609http://purl.uniprot.org/core/author"Li J."xsd:string
http://purl.uniprot.org/citations/29482609http://purl.uniprot.org/core/author"Liu X."xsd:string
http://purl.uniprot.org/citations/29482609http://purl.uniprot.org/core/author"Li J.'"xsd:string
http://purl.uniprot.org/citations/29482609http://purl.uniprot.org/core/author"Song W."xsd:string
http://purl.uniprot.org/citations/29482609http://purl.uniprot.org/core/author"Zhang J."xsd:string
http://purl.uniprot.org/citations/29482609http://purl.uniprot.org/core/author"Zhou X."xsd:string
http://purl.uniprot.org/citations/29482609http://purl.uniprot.org/core/author"Zhou H."xsd:string
http://purl.uniprot.org/citations/29482609http://purl.uniprot.org/core/author"Wang Z."xsd:string
http://purl.uniprot.org/citations/29482609http://purl.uniprot.org/core/author"Wang K."xsd:string
http://purl.uniprot.org/citations/29482609http://purl.uniprot.org/core/author"Zou C."xsd:string
http://purl.uniprot.org/citations/29482609http://purl.uniprot.org/core/author"Xie R."xsd:string
http://purl.uniprot.org/citations/29482609http://purl.uniprot.org/core/author"Ma T."xsd:string
http://purl.uniprot.org/citations/29482609http://purl.uniprot.org/core/author"Bian C."xsd:string
http://purl.uniprot.org/citations/29482609http://purl.uniprot.org/core/author"Xin C."xsd:string
http://purl.uniprot.org/citations/29482609http://purl.uniprot.org/core/author"Cui K."xsd:string
http://purl.uniprot.org/citations/29482609http://purl.uniprot.org/core/author"Wei Y.Q."xsd:string
http://purl.uniprot.org/citations/29482609http://purl.uniprot.org/core/author"Cen Z."xsd:string
http://purl.uniprot.org/citations/29482609http://purl.uniprot.org/core/date"2018"xsd:gYear
http://purl.uniprot.org/citations/29482609http://purl.uniprot.org/core/name"Arthritis Res Ther"xsd:string
http://purl.uniprot.org/citations/29482609http://purl.uniprot.org/core/pages"34"xsd:string
http://purl.uniprot.org/citations/29482609http://purl.uniprot.org/core/title"MicroRNA-302b negatively regulates IL-1beta production in response to MSU crystals by targeting IRAK4 and EphA2."xsd:string
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