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http://purl.uniprot.org/citations/29499182http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/29499182http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/29499182http://www.w3.org/2000/01/rdf-schema#comment"T antigen (Galβ1-3GalNAcα1-Ser/Thr) is an evolutionary-conserved mucin-type core 1 glycan structure in animals synthesized by core 1 β1,3-galactosyltransferase 1 (C1GalT1). Previous studies showed that T antigen produced by Drosophila C1GalT1 (dC1GalT1) was expressed in various tissues and dC1GalT1 loss in larvae led to various defects, including decreased number of circulating hemocytes, hyper-differentiation of hematopoietic stem cells in lymph glands, malformation of the central nervous system, mislocalization of neuromuscular junction (NMJ) boutons, and ultrastructural abnormalities in NMJs and muscle cells. Although glucuronylated T antigen (GlcAβ1-3Galβ1-3GalNAcα1-Ser/Thr) has been identified in Drosophila, the physiological function of this structure has not yet been clarified. In this study, for the first time, we unraveled biological roles of glucuronylated T antigen. Our data show that in Drosophila, glucuronylation of T antigen is predominantly carried out by Drosophila β1,3-glucuronyltransferase-P (dGlcAT-P). We created dGlcAT-P null mutants and found that mutant larvae showed lower expression of glucuronylated T antigen on the muscles and at NMJs. Furthermore, mislocalization of NMJ boutons and a partial loss of the basement membrane components collagen IV (Col IV) and nidogen (Ndg) at the muscle 6/7 boundary were observed. Those two phenotypes were correlated and identical to previously described phenotypes in dC1GalT1 mutant larvae. In addition, dGlcAT-P null mutants exhibited fewer NMJ branches on muscles 6/7. Moreover, ultrastructural analysis revealed that basement membranes that lacked Col IV and Ndg were significantly deformed. We also found that the loss of dGlcAT-P expression caused ultrastructural defects in NMJ boutons. Finally, we showed a genetic interaction between dGlcAT-P and dC1GalT1. Therefore, these results demonstrate that glucuronylated core 1 glycans synthesized by dGlcAT-P are key modulators of NMJ bouton localization, basement membrane formation, and NMJ arborization on larval muscles."xsd:string
http://purl.uniprot.org/citations/29499182http://purl.org/dc/terms/identifier"doi:10.1016/j.ydbio.2018.02.017"xsd:string
http://purl.uniprot.org/citations/29499182http://purl.uniprot.org/core/author"Aoki K."xsd:string
http://purl.uniprot.org/citations/29499182http://purl.uniprot.org/core/author"Aoki K."xsd:string
http://purl.uniprot.org/citations/29499182http://purl.uniprot.org/core/author"Itoh K."xsd:string
http://purl.uniprot.org/citations/29499182http://purl.uniprot.org/core/author"Itoh K."xsd:string
http://purl.uniprot.org/citations/29499182http://purl.uniprot.org/core/author"Kondo S."xsd:string
http://purl.uniprot.org/citations/29499182http://purl.uniprot.org/core/author"Kondo S."xsd:string
http://purl.uniprot.org/citations/29499182http://purl.uniprot.org/core/author"Akimoto Y."xsd:string
http://purl.uniprot.org/citations/29499182http://purl.uniprot.org/core/author"Akimoto Y."xsd:string
http://purl.uniprot.org/citations/29499182http://purl.uniprot.org/core/author"Tiemeyer M."xsd:string
http://purl.uniprot.org/citations/29499182http://purl.uniprot.org/core/author"Tiemeyer M."xsd:string
http://purl.uniprot.org/citations/29499182http://purl.uniprot.org/core/author"Nishihara S."xsd:string
http://purl.uniprot.org/citations/29499182http://purl.uniprot.org/core/author"Nishihara S."xsd:string
http://purl.uniprot.org/citations/29499182http://purl.uniprot.org/core/author"Ichimiya T."xsd:string
http://purl.uniprot.org/citations/29499182http://purl.uniprot.org/core/author"Ichimiya T."xsd:string
http://purl.uniprot.org/citations/29499182http://purl.uniprot.org/core/date"2018"xsd:gYear
http://purl.uniprot.org/citations/29499182http://purl.uniprot.org/core/date"2018"xsd:gYear
http://purl.uniprot.org/citations/29499182http://purl.uniprot.org/core/name"Dev. Biol."xsd:string
http://purl.uniprot.org/citations/29499182http://purl.uniprot.org/core/name"Dev Biol"xsd:string
http://purl.uniprot.org/citations/29499182http://purl.uniprot.org/core/pages"108-124"xsd:string
http://purl.uniprot.org/citations/29499182http://purl.uniprot.org/core/pages"108-124"xsd:string
http://purl.uniprot.org/citations/29499182http://purl.uniprot.org/core/title"Glucuronylated core 1 glycans are required for precise localization of neuromuscular junctions and normal formation of basement membranes on Drosophila muscles."xsd:string
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