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http://purl.uniprot.org/citations/29574636http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/29574636http://www.w3.org/2000/01/rdf-schema#comment"

Purpose

G protein-coupled receptors (GPCRs) represent the largest family of druggable targets in human genome. Although several GPCRs can cross-talk with the human epidermal growth factor receptors (HERs), the expression and function of most GPCRs remain unknown in HER2+ breast cancer (BC). In this study, we aimed to evaluate gene expression of GPCRs in tumorigenic or anti-HER2 drug-resistant cells and to understand the potential role of candidate GPCRs in HER2+ BC.

Methods

Gene expression of 352 GPCRs was profiled in Aldeflur+ tumorigenic versus Aldeflur-population and anti-HER2 therapy-resistant derivatives versus parental cells of HER2+ BT474 cells. The GPCR candidates were confirmed in 7 additional HER2+ BC cell line models and publicly available patient dataset. Anchorage-dependent and anchorage-independent cell growth, mammosphere formation, and migration/invasion were evaluated upon GPR110 knockdown by siRNA in BT474 and SKBR3 parental and lapatinib+ trastuzumab-resistant (LTR) cells.

Results

Adhesion and class A GPCRs were overexpressed in Aldeflur+ and anti-HER2 therapy-resistant population of BT474 cells, respectively. GPR110 was the only GPCR overexpressed in Aldeflur+ and anti-HER2 therapy-resistant population in BT474, SKBR3, HCC1569, MDA-MB-361, AU565, and/or HCC202 cells and in HER2+ BC subtype in patient tumors. Using BT474 and SKBR3 parental and LTR cells, we found that GPR110 knockdown significantly reduced anchorage-dependent/independent cell growth as well as migration/invasion of parental and LTR cells and mammosphere formation in LTR derivatives and not in parental cells.

Conclusion

Our data suggest a potential role of GPR110 in tumorigenicity and in tumor cell dissemination in HER2+ BC."xsd:string
http://purl.uniprot.org/citations/29574636http://purl.org/dc/terms/identifier"doi:10.1007/s10549-018-4751-9"xsd:string
http://purl.uniprot.org/citations/29574636http://purl.uniprot.org/core/author"Fu X."xsd:string
http://purl.uniprot.org/citations/29574636http://purl.uniprot.org/core/author"Yadav V."xsd:string
http://purl.uniprot.org/citations/29574636http://purl.uniprot.org/core/author"Qin L."xsd:string
http://purl.uniprot.org/citations/29574636http://purl.uniprot.org/core/author"Creighton C.J."xsd:string
http://purl.uniprot.org/citations/29574636http://purl.uniprot.org/core/author"Yadav P."xsd:string
http://purl.uniprot.org/citations/29574636http://purl.uniprot.org/core/author"Nanda S."xsd:string
http://purl.uniprot.org/citations/29574636http://purl.uniprot.org/core/author"De Angelis C."xsd:string
http://purl.uniprot.org/citations/29574636http://purl.uniprot.org/core/author"Osborne C.K."xsd:string
http://purl.uniprot.org/citations/29574636http://purl.uniprot.org/core/author"Schiff R."xsd:string
http://purl.uniprot.org/citations/29574636http://purl.uniprot.org/core/author"Narkar V.A."xsd:string
http://purl.uniprot.org/citations/29574636http://purl.uniprot.org/core/author"Bhargava D.K."xsd:string
http://purl.uniprot.org/citations/29574636http://purl.uniprot.org/core/author"Sahay D."xsd:string
http://purl.uniprot.org/citations/29574636http://purl.uniprot.org/core/author"Trivedi M.V."xsd:string
http://purl.uniprot.org/citations/29574636http://purl.uniprot.org/core/author"Sethunath V."xsd:string
http://purl.uniprot.org/citations/29574636http://purl.uniprot.org/core/author"Al-Rawi A."xsd:string
http://purl.uniprot.org/citations/29574636http://purl.uniprot.org/core/author"Bhat R.R."xsd:string
http://purl.uniprot.org/citations/29574636http://purl.uniprot.org/core/author"Yazdanfard S."xsd:string
http://purl.uniprot.org/citations/29574636http://purl.uniprot.org/core/date"2018"xsd:gYear
http://purl.uniprot.org/citations/29574636http://purl.uniprot.org/core/name"Breast Cancer Res Treat"xsd:string
http://purl.uniprot.org/citations/29574636http://purl.uniprot.org/core/pages"279-292"xsd:string
http://purl.uniprot.org/citations/29574636http://purl.uniprot.org/core/title"GPCRs profiling and identification of GPR110 as a potential new target in HER2+ breast cancer."xsd:string
http://purl.uniprot.org/citations/29574636http://purl.uniprot.org/core/volume"170"xsd:string
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