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http://purl.uniprot.org/citations/29598828http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/29598828http://www.w3.org/2000/01/rdf-schema#comment"

Background

Eukaryotic transcription is regulated through two complexes, the general transcription factor IID (TFIID) and the coactivator Spt-Ada-Gcn5 acetyltransferase (SAGA). Recent findings confirm that both TFIID and SAGA contribute to the synthesis of nearly all transcripts and are recruited genome-wide in yeast. However, how this broad recruitment confers selectivity under specific conditions remains an open question.

Results

Here we find that the SAGA/TREX-2 subunit Sus1 associates with upstream regulatory regions of many yeast genes and that heat shock drastically changes Sus1 binding. While Sus1 binding to TFIID-dominated genes is not affected by temperature, its recruitment to SAGA-dominated genes and RP genes is significantly disturbed under heat shock, with Sus1 relocated to environmental stress-responsive genes in these conditions. Moreover, in contrast to recent results showing that SAGA deubiquitinating enzyme Ubp8 is dispensable for RNA synthesis, genomic run-on experiments demonstrate that Sus1 contributes to synthesis and stability of a wide range of transcripts.

Conclusions

Our study provides support for a model in which SAGA/TREX-2 factor Sus1 acts as a global transcriptional regulator in yeast but has differential activity at yeast genes as a function of their transcription rate or during stress conditions."xsd:string
http://purl.uniprot.org/citations/29598828http://purl.org/dc/terms/identifier"doi:10.1186/s13072-018-0184-2"xsd:string
http://purl.uniprot.org/citations/29598828http://purl.uniprot.org/core/author"Perez-Ortin J.E."xsd:string
http://purl.uniprot.org/citations/29598828http://purl.uniprot.org/core/author"Rodriguez-Navarro S."xsd:string
http://purl.uniprot.org/citations/29598828http://purl.uniprot.org/core/author"Garcia-Martinez J."xsd:string
http://purl.uniprot.org/citations/29598828http://purl.uniprot.org/core/author"Pugh B.F."xsd:string
http://purl.uniprot.org/citations/29598828http://purl.uniprot.org/core/author"Antunez O."xsd:string
http://purl.uniprot.org/citations/29598828http://purl.uniprot.org/core/author"Reja R."xsd:string
http://purl.uniprot.org/citations/29598828http://purl.uniprot.org/core/author"Conesa A."xsd:string
http://purl.uniprot.org/citations/29598828http://purl.uniprot.org/core/author"Garcia-Molinero V."xsd:string
http://purl.uniprot.org/citations/29598828http://purl.uniprot.org/core/author"Vinayachandran V."xsd:string
http://purl.uniprot.org/citations/29598828http://purl.uniprot.org/core/author"Furio-Tari P."xsd:string
http://purl.uniprot.org/citations/29598828http://purl.uniprot.org/core/date"2018"xsd:gYear
http://purl.uniprot.org/citations/29598828http://purl.uniprot.org/core/name"Epigenetics Chromatin"xsd:string
http://purl.uniprot.org/citations/29598828http://purl.uniprot.org/core/pages"13"xsd:string
http://purl.uniprot.org/citations/29598828http://purl.uniprot.org/core/title"The SAGA/TREX-2 subunit Sus1 binds widely to transcribed genes and affects mRNA turnover globally."xsd:string
http://purl.uniprot.org/citations/29598828http://purl.uniprot.org/core/volume"11"xsd:string
http://purl.uniprot.org/citations/29598828http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/29598828
http://purl.uniprot.org/citations/29598828http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/29598828
http://purl.uniprot.org/uniprot/#_Q6WNK7-mappedCitation-29598828http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/29598828
http://purl.uniprot.org/uniprot/Q6WNK7http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/29598828