| http://purl.uniprot.org/citations/29630591 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/29630591 | http://www.w3.org/2000/01/rdf-schema#comment | "Nitric oxide (NO) regulates neuronal function and thus is critical for tuning neuronal communication. Mechanisms by which NO modulates protein function and interaction include posttranslational modifications (PTMs) such as S-nitrosylation. Importantly, cross signaling between S-nitrosylation and prenylation can have major regulatory potential. However, the exact protein targets and resulting changes in function remain elusive. Here, we interrogated the role of NO-dependent PTMs and farnesylation in synaptic transmission. We found that NO compromises synaptic function at the Drosophila neuromuscular junction (NMJ) in a cGMP-independent manner. NO suppressed release and reduced the size of available vesicle pools, which was reversed by glutathione (GSH) and occluded by genetic up-regulation of GSH-generating and de-nitrosylating glutamate-cysteine-ligase and S-nitroso-glutathione reductase activities. Enhanced nitrergic activity led to S-nitrosylation of the fusion-clamp protein complexin (cpx) and altered its membrane association and interactions with active zone (AZ) and soluble N-ethyl-maleimide-sensitive fusion protein Attachment Protein Receptor (SNARE) proteins. Furthermore, genetic and pharmacological suppression of farnesylation and a nitrosylation mimetic mutant of cpx induced identical physiological and localization phenotypes as caused by NO. Together, our data provide evidence for a novel physiological nitrergic molecular switch involving S-nitrosylation, which reversibly suppresses farnesylation and thereby enhances the net-clamping function of cpx. These data illustrate a new mechanistic signaling pathway by which regulation of farnesylation can fine-tune synaptic release."xsd:string |
| http://purl.uniprot.org/citations/29630591 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pbio.2003611"xsd:string |
| http://purl.uniprot.org/citations/29630591 | http://purl.uniprot.org/core/author | "Smith T.M."xsd:string |
| http://purl.uniprot.org/citations/29630591 | http://purl.uniprot.org/core/author | "Mistry R."xsd:string |
| http://purl.uniprot.org/citations/29630591 | http://purl.uniprot.org/core/author | "Breda C."xsd:string |
| http://purl.uniprot.org/citations/29630591 | http://purl.uniprot.org/core/author | "Robinson S.W."xsd:string |
| http://purl.uniprot.org/citations/29630591 | http://purl.uniprot.org/core/author | "Morone N."xsd:string |
| http://purl.uniprot.org/citations/29630591 | http://purl.uniprot.org/core/author | "Giorgini F."xsd:string |
| http://purl.uniprot.org/citations/29630591 | http://purl.uniprot.org/core/author | "Dinsdale D."xsd:string |
| http://purl.uniprot.org/citations/29630591 | http://purl.uniprot.org/core/author | "Mallucci G.R."xsd:string |
| http://purl.uniprot.org/citations/29630591 | http://purl.uniprot.org/core/author | "Campesan S."xsd:string |
| http://purl.uniprot.org/citations/29630591 | http://purl.uniprot.org/core/author | "Butcher A."xsd:string |
| http://purl.uniprot.org/citations/29630591 | http://purl.uniprot.org/core/author | "Steinert J.R."xsd:string |
| http://purl.uniprot.org/citations/29630591 | http://purl.uniprot.org/core/author | "Bourgognon J.M."xsd:string |
| http://purl.uniprot.org/citations/29630591 | http://purl.uniprot.org/core/author | "Challiss R.A.J."xsd:string |
| http://purl.uniprot.org/citations/29630591 | http://purl.uniprot.org/core/author | "Guerra-Martin M."xsd:string |
| http://purl.uniprot.org/citations/29630591 | http://purl.uniprot.org/core/author | "Spiers J.G."xsd:string |
| http://purl.uniprot.org/citations/29630591 | http://purl.uniprot.org/core/date | "2018"xsd:gYear |
| http://purl.uniprot.org/citations/29630591 | http://purl.uniprot.org/core/name | "PLoS Biol"xsd:string |
| http://purl.uniprot.org/citations/29630591 | http://purl.uniprot.org/core/pages | "e2003611"xsd:string |
| http://purl.uniprot.org/citations/29630591 | http://purl.uniprot.org/core/title | "Nitric oxide-mediated posttranslational modifications control neurotransmitter release by modulating complexin farnesylation and enhancing its clamping ability."xsd:string |
| http://purl.uniprot.org/citations/29630591 | http://purl.uniprot.org/core/volume | "16"xsd:string |
| http://purl.uniprot.org/citations/29630591 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/29630591 |
| http://purl.uniprot.org/citations/29630591 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/29630591 |