| http://purl.uniprot.org/citations/29684280 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/29684280 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/29684280 | http://www.w3.org/2000/01/rdf-schema#comment | "The substrate profiles for three uncharacterized enzymes (YcjM, YcjT, and YcjU) that are expressed from a cluster of 12 genes ( ycjM-W and ompG) of unknown function in Escherichia coli K-12 were determined. Through a comprehensive bioinformatic and steady-state kinetic analysis, the catalytic function of YcjT was determined to be kojibiose phosphorylase. In the presence of saturating phosphate and kojibiose (α-(1,2)-d-glucose-d-glucose), this enzyme catalyzes the formation of d-glucose and β-d-glucose-1-phosphate ( kcat = 1.1 s-1, Km = 1.05 mM, and kcat/ Km = 1.12 × 103 M-1 s-1). Additionally, it was also shown that in the presence of β-d-glucose-1-phosphate, YcjT can catalyze the formation of other disaccharides using 1,5-anhydro-d-glucitol, l-sorbose, d-sorbitol, or l-iditol as a substitute for d-glucose. Kojibiose is a component of cell wall lipoteichoic acids in Gram-positive bacteria and is of interest as a potential low-calorie sweetener and prebiotic. YcjU was determined to be a β-phosphoglucomutase that catalyzes the isomerization of β-d-glucose-1-phosphate ( kcat = 21 s-1, Km = 18 μM, and kcat/ Km = 1.1 × 106 M-1 s-1) to d-glucose-6-phosphate. YcjU was also shown to exhibit catalytic activity with β-d-allose-1-phosphate, β-d-mannose-1-phosphate, and β-d-galactose-1-phosphate. YcjM catalyzes the phosphorolysis of α-(1,2)-d-glucose-d-glycerate with a kcat = 2.1 s-1, Km = 69 μM, and kcat/ Km = 3.1 × 104 M-1 s-1."xsd:string |
| http://purl.uniprot.org/citations/29684280 | http://purl.org/dc/terms/identifier | "doi:10.1021/acs.biochem.8b00392"xsd:string |
| http://purl.uniprot.org/citations/29684280 | http://purl.org/dc/terms/identifier | "doi:10.1021/acs.biochem.8b00392"xsd:string |
| http://purl.uniprot.org/citations/29684280 | http://purl.uniprot.org/core/author | "Raushel F.M."xsd:string |
| http://purl.uniprot.org/citations/29684280 | http://purl.uniprot.org/core/author | "Raushel F.M."xsd:string |
| http://purl.uniprot.org/citations/29684280 | http://purl.uniprot.org/core/author | "Narindoshvili T."xsd:string |
| http://purl.uniprot.org/citations/29684280 | http://purl.uniprot.org/core/author | "Narindoshvili T."xsd:string |
| http://purl.uniprot.org/citations/29684280 | http://purl.uniprot.org/core/author | "Mukherjee K."xsd:string |
| http://purl.uniprot.org/citations/29684280 | http://purl.uniprot.org/core/author | "Mukherjee K."xsd:string |
| http://purl.uniprot.org/citations/29684280 | http://purl.uniprot.org/core/date | "2018"xsd:gYear |
| http://purl.uniprot.org/citations/29684280 | http://purl.uniprot.org/core/date | "2018"xsd:gYear |
| http://purl.uniprot.org/citations/29684280 | http://purl.uniprot.org/core/name | "Biochemistry"xsd:string |
| http://purl.uniprot.org/citations/29684280 | http://purl.uniprot.org/core/name | "Biochemistry"xsd:string |
| http://purl.uniprot.org/citations/29684280 | http://purl.uniprot.org/core/pages | "2857-2867"xsd:string |
| http://purl.uniprot.org/citations/29684280 | http://purl.uniprot.org/core/pages | "2857-2867"xsd:string |
| http://purl.uniprot.org/citations/29684280 | http://purl.uniprot.org/core/title | "Discovery of a kojibiose phosphorylase in Escherichia coli K-12."xsd:string |
| http://purl.uniprot.org/citations/29684280 | http://purl.uniprot.org/core/title | "Discovery of a kojibiose phosphorylase in Escherichia coli K-12."xsd:string |
| http://purl.uniprot.org/citations/29684280 | http://purl.uniprot.org/core/volume | "57"xsd:string |
| http://purl.uniprot.org/citations/29684280 | http://purl.uniprot.org/core/volume | "57"xsd:string |
| http://purl.uniprot.org/citations/29684280 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/29684280 |
| http://purl.uniprot.org/citations/29684280 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/29684280 |
| http://purl.uniprot.org/citations/29684280 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/29684280 |
| http://purl.uniprot.org/citations/29684280 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/29684280 |