| http://purl.uniprot.org/citations/29694914 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/29694914 | http://www.w3.org/2000/01/rdf-schema#comment | "GSK3β interacting protein (GSKIP) is a naturally occurring negative regulator of GSK3β and retains both the Protein Kinase A Regulatory subunit binding (PKA-RII) domain and GSK3β interacting domain. Of these two domains, we found that PKA-RII is required for forming a working complex comprising PKA/GSKIP/GSK3β/Drp1 to influence phosphorylation of Drp1 Ser637. In this study, bioinformatics and experimental explorations re-analyzing GSKIP's biofunctions suggest that the evolutionarily conserved Domain of Unknown Function (DUF727) is an ancestral prototype of GSKIP in prokaryotes, and acquired the C-terminal GSK3β binding site (tail) in invertebrates except for Saccharomyces spp., after which the N-terminal PKA-RII binding region (head) evolved in vertebrates. These two regions mutually influence each other and modulate GSKIP binding to GSK3β in yeast two-hybrid assays and co-immunoprecipitation. Molecular modeling showed that mammalian GSKIP could form a dimer through the L130 residue (GSK3β binding site) rather than V41/L45 residues. In contrast, V41/L45P mutant facilitated a gain-of-function effect on GSKIP dimerization, further influencing binding behavior to GSK3β compared to GSKIP wild-type (wt). The V41/L45 residues are not only responsible for PKA RII binding that controls GSK3β activity, but also affect dimerization of GSKIP monomer, with net results of gain-of-function in GSKIP-GSK3β interaction. In addition to its reported role in modulating Drp1, Ser637 phosphorylation caused mitochondrial elongation; we postulated that GSKIP might be involved in the Wnt signaling pathway as a scavenger to recruit GSK3β away from the β-catenin destruction complex and as a competitor to compete for GSK3β binding, resulting in accumulation of S675 phosphorylated β-catenin."xsd:string |
| http://purl.uniprot.org/citations/29694914 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bbamcr.2018.04.008"xsd:string |
| http://purl.uniprot.org/citations/29694914 | http://purl.uniprot.org/core/author | "Wang Y.H."xsd:string |
| http://purl.uniprot.org/citations/29694914 | http://purl.uniprot.org/core/author | "Tseng C.N."xsd:string |
| http://purl.uniprot.org/citations/29694914 | http://purl.uniprot.org/core/author | "Yang M.C."xsd:string |
| http://purl.uniprot.org/citations/29694914 | http://purl.uniprot.org/core/author | "Chuang Y.C."xsd:string |
| http://purl.uniprot.org/citations/29694914 | http://purl.uniprot.org/core/author | "Chou C.H."xsd:string |
| http://purl.uniprot.org/citations/29694914 | http://purl.uniprot.org/core/author | "Cheng J.T."xsd:string |
| http://purl.uniprot.org/citations/29694914 | http://purl.uniprot.org/core/author | "Hong Y.R."xsd:string |
| http://purl.uniprot.org/citations/29694914 | http://purl.uniprot.org/core/author | "Howng S.L."xsd:string |
| http://purl.uniprot.org/citations/29694914 | http://purl.uniprot.org/core/author | "Lieu A.S."xsd:string |
| http://purl.uniprot.org/citations/29694914 | http://purl.uniprot.org/core/author | "Loh J.K."xsd:string |
| http://purl.uniprot.org/citations/29694914 | http://purl.uniprot.org/core/author | "Chou A.K."xsd:string |
| http://purl.uniprot.org/citations/29694914 | http://purl.uniprot.org/core/author | "Liu H.F."xsd:string |
| http://purl.uniprot.org/citations/29694914 | http://purl.uniprot.org/core/author | "Yang C.N."xsd:string |
| http://purl.uniprot.org/citations/29694914 | http://purl.uniprot.org/core/author | "Chiou S.J."xsd:string |
| http://purl.uniprot.org/citations/29694914 | http://purl.uniprot.org/core/author | "Hsiao B.X."xsd:string |
| http://purl.uniprot.org/citations/29694914 | http://purl.uniprot.org/core/date | "2018"xsd:gYear |
| http://purl.uniprot.org/citations/29694914 | http://purl.uniprot.org/core/name | "Biochim Biophys Acta Mol Cell Res"xsd:string |
| http://purl.uniprot.org/citations/29694914 | http://purl.uniprot.org/core/pages | "1046-1059"xsd:string |
| http://purl.uniprot.org/citations/29694914 | http://purl.uniprot.org/core/title | "The origin of GSKIP, a multifaceted regulatory factor in the mammalian Wnt pathway."xsd:string |
| http://purl.uniprot.org/citations/29694914 | http://purl.uniprot.org/core/volume | "1865"xsd:string |
| http://purl.uniprot.org/citations/29694914 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/29694914 |
| http://purl.uniprot.org/citations/29694914 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/29694914 |