| http://purl.uniprot.org/citations/29724861 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/29724861 | http://www.w3.org/2000/01/rdf-schema#comment | "Post-translational control is a crucial mechanism for circadian timekeeping. Evolutionarily conserved kinases and phosphatases have been implicated in circadian phosphorylation and the degradation of clock-relevant proteins, which sustain high-amplitude rhythms with 24-hr periodicity in animal behaviors and physiology. Here, we report a novel clock function of the heterodimeric Ca2+/calmodulin-dependent phosphatase calcineurin and its regulator sarah (sra) in Drosophila Genomic deletion of the sra locus dampened circadian locomotor activity rhythms in free-running constant dark after entrainment in light-dark cycles. Poor rhythms in sra mutant behaviors were accompanied by lower expression of two oscillating clock proteins, PERIOD (PER) and TIMELESS (TIM), at the post-transcriptional level. RNA interference-mediated sra depletion in circadian pacemaker neurons was sufficient to phenocopy loss-of-function mutation in sra On the other hand, a constitutively active form of the catalytic calcineurin subunit, Pp2B-14DACT, shortened circadian periodicity in locomotor behaviors and phase-advanced PER and TIM rhythms when overexpressed in clock neurons. Heterozygous sra deletion induced behavioral arrhythmicity in Pp2B-14DACT flies, whereas sra overexpression rescued short periods in these animals. Finally, pharmacological inhibition of calcineurin in either wild-type flies or clock-less S2 cells decreased the levels of PER and TIM, likely by facilitating their proteasomal degradation. Taken together, these data suggest that sra negatively regulates calcineurin by cell-autonomously titrating calcineurin-dependent stabilization of PER and TIM proteins, thereby sustaining high-amplitude behavioral rhythms in Drosophila."xsd:string |
| http://purl.uniprot.org/citations/29724861 | http://purl.org/dc/terms/identifier | "doi:10.1534/genetics.118.300808"xsd:string |
| http://purl.uniprot.org/citations/29724861 | http://purl.uniprot.org/core/author | "Lee J."xsd:string |
| http://purl.uniprot.org/citations/29724861 | http://purl.uniprot.org/core/author | "Choe J."xsd:string |
| http://purl.uniprot.org/citations/29724861 | http://purl.uniprot.org/core/author | "Lim C."xsd:string |
| http://purl.uniprot.org/citations/29724861 | http://purl.uniprot.org/core/author | "Kweon S.H."xsd:string |
| http://purl.uniprot.org/citations/29724861 | http://purl.uniprot.org/core/date | "2018"xsd:gYear |
| http://purl.uniprot.org/citations/29724861 | http://purl.uniprot.org/core/name | "Genetics"xsd:string |
| http://purl.uniprot.org/citations/29724861 | http://purl.uniprot.org/core/pages | "815-828"xsd:string |
| http://purl.uniprot.org/citations/29724861 | http://purl.uniprot.org/core/title | "High-Amplitude Circadian Rhythms in Drosophila Driven by Calcineurin-Mediated Post-translational Control of sarah."xsd:string |
| http://purl.uniprot.org/citations/29724861 | http://purl.uniprot.org/core/volume | "209"xsd:string |
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| http://purl.uniprot.org/uniprot/#_M9NEL1-mappedCitation-29724861 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/29724861 |
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| http://purl.uniprot.org/uniprot/#_M9NE01-mappedCitation-29724861 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/29724861 |