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http://purl.uniprot.org/citations/29767755http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/29767755http://www.w3.org/2000/01/rdf-schema#comment"Potassium (K+) is a major macronutrient required for plant growth. An adaptive mechanism to low-K+ conditions involves activation of the Ca2+ signaling network that consists of calcineurin B-like proteins (CBLs) and CBL-interacting kinases (CIPKs). The CBL-interacting protein kinase 9 (CIPK9) has previously been implicated in low-K+ responses in Arabidopsis thaliana. Here, we report a protein phosphatase 2C (PP2C), AP2C1, that interacts with CIPK9. Fluorescence resonance energy transfer (FRET), bimolecular fluorescence complementation (BiFC), and co-localization analyses revealed that CIPK9 and AP2C1 interact in the cytoplasm. AP2C1 dephosphorylates the auto-phosphorylated form of CIPK9 in vitro, presenting a regulatory mechanism for CIPK9 function. Furthermore, genetic and molecular analyses revealed that ap2c1 null mutants (ap2c1-1 and ap2c1-2) are tolerant to low-K+ conditions, retain higher K+ content, and show higher expression of K+-deficiency related genes contrary to cipk9 mutants (cipk9-1 and cipk9-2). In contrast, transgenic plants overexpressing AP2C1 were sensitive to low-K+ conditions. Thus, this study shows that AP2C1 and CIPK9 interact to regulate K+-deficiency responses in Arabidopsis. CIPK9 functions as positive regulator whereas AP2C1 acts as a negative regulator of Arabidopsis root growth and seedling development under low-K+ conditions."xsd:string
http://purl.uniprot.org/citations/29767755http://purl.org/dc/terms/identifier"doi:10.1093/jxb/ery182"xsd:string
http://purl.uniprot.org/citations/29767755http://purl.uniprot.org/core/author"Singh A."xsd:string
http://purl.uniprot.org/citations/29767755http://purl.uniprot.org/core/author"Kaur K."xsd:string
http://purl.uniprot.org/citations/29767755http://purl.uniprot.org/core/author"Pandey A."xsd:string
http://purl.uniprot.org/citations/29767755http://purl.uniprot.org/core/author"Yadav A.K."xsd:string
http://purl.uniprot.org/citations/29767755http://purl.uniprot.org/core/author"Sharma P."xsd:string
http://purl.uniprot.org/citations/29767755http://purl.uniprot.org/core/author"Luan S."xsd:string
http://purl.uniprot.org/citations/29767755http://purl.uniprot.org/core/author"Jha S.K."xsd:string
http://purl.uniprot.org/citations/29767755http://purl.uniprot.org/core/author"Pandey G.K."xsd:string
http://purl.uniprot.org/citations/29767755http://purl.uniprot.org/core/author"Fernandes J.L."xsd:string
http://purl.uniprot.org/citations/29767755http://purl.uniprot.org/core/author"Sanyal S.K."xsd:string
http://purl.uniprot.org/citations/29767755http://purl.uniprot.org/core/author"Tokas I."xsd:string
http://purl.uniprot.org/citations/29767755http://purl.uniprot.org/core/date"2018"xsd:gYear
http://purl.uniprot.org/citations/29767755http://purl.uniprot.org/core/name"J Exp Bot"xsd:string
http://purl.uniprot.org/citations/29767755http://purl.uniprot.org/core/pages"4003-4015"xsd:string
http://purl.uniprot.org/citations/29767755http://purl.uniprot.org/core/title"A protein phosphatase 2C, AP2C1, interacts with and negatively regulates the function of CIPK9 under potassium-deficient conditions in Arabidopsis."xsd:string
http://purl.uniprot.org/citations/29767755http://purl.uniprot.org/core/volume"69"xsd:string
http://purl.uniprot.org/citations/29767755http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/29767755
http://purl.uniprot.org/citations/29767755http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/29767755
http://purl.uniprot.org/uniprot/#_A0A178W7T6-mappedCitation-29767755http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/29767755
http://purl.uniprot.org/uniprot/#_A0A5S9X2J0-mappedCitation-29767755http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/29767755
http://purl.uniprot.org/uniprot/#_F6LPR5-mappedCitation-29767755http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/29767755
http://purl.uniprot.org/uniprot/#_O80871-mappedCitation-29767755http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/29767755