| http://purl.uniprot.org/citations/29807321 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/29807321 | http://www.w3.org/2000/01/rdf-schema#comment | "Polynucleotide kinase/phosphatase (PNKP) has been implicated in non-homologous end joining (NHEJ) of DNA double-strand breaks (DSBs). To assess the consequences of PNKP deficiency for NHEJ of 3'-phosphate-ended DSBs, PNKP-deficient derivatives of HCT116 and of HeLa cells were generated using CRISPR/CAS9. For both cell lines, PNKP deficiency conferred sensitivity to ionizing radiation as well as to neocarzinostatin (NCS), which specifically induces DSBs bearing protruding 3'-phosphate termini. Moreover, NCS-induced DSBs, detected as 53BP1 foci, were more persistent in PNKP -/- HCT116 cells compared to their wild-type (WT) counterparts. Surprisingly, PNKP-deficient whole-cell and nuclear extracts were biochemically competent in removing both protruding and recessed 3'-phosphates from synthetic DSB substrates, albeit much less efficiently than WT extracts, suggesting an alternative 3'-phosphatase. Measurements by ligation-mediated PCR showed that PNKP-deficient HeLa cells contained significantly more 3'-phosphate-terminated and fewer 3'-hydroxyl-terminated DSBs than parental cells 5-15 min after NCS treatment, but this difference disappeared by 1 h. These results suggest that, despite presence of an alternative 3'-phosphatase, loss of PNKP significantly sensitizes cells to 3'-phosphate-terminated DSBs, due to a 3'-dephosphorylation defect."xsd:string |
| http://purl.uniprot.org/citations/29807321 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.dnarep.2018.05.002"xsd:string |
| http://purl.uniprot.org/citations/29807321 | http://purl.uniprot.org/core/author | "Yu Y."xsd:string |
| http://purl.uniprot.org/citations/29807321 | http://purl.uniprot.org/core/author | "Weinfeld M."xsd:string |
| http://purl.uniprot.org/citations/29807321 | http://purl.uniprot.org/core/author | "Akopiants K."xsd:string |
| http://purl.uniprot.org/citations/29807321 | http://purl.uniprot.org/core/author | "Povirk L.F."xsd:string |
| http://purl.uniprot.org/citations/29807321 | http://purl.uniprot.org/core/author | "Fanta M."xsd:string |
| http://purl.uniprot.org/citations/29807321 | http://purl.uniprot.org/core/author | "Kawale A.S."xsd:string |
| http://purl.uniprot.org/citations/29807321 | http://purl.uniprot.org/core/author | "Chalasani S.L."xsd:string |
| http://purl.uniprot.org/citations/29807321 | http://purl.uniprot.org/core/date | "2018"xsd:gYear |
| http://purl.uniprot.org/citations/29807321 | http://purl.uniprot.org/core/name | "DNA Repair (Amst)"xsd:string |
| http://purl.uniprot.org/citations/29807321 | http://purl.uniprot.org/core/pages | "12-24"xsd:string |
| http://purl.uniprot.org/citations/29807321 | http://purl.uniprot.org/core/title | "Persistent 3'-phosphate termini and increased cytotoxicity of radiomimetic DNA double-strand breaks in cells lacking polynucleotide kinase/phosphatase despite presence of an alternative 3'-phosphatase."xsd:string |
| http://purl.uniprot.org/citations/29807321 | http://purl.uniprot.org/core/volume | "68"xsd:string |
| http://purl.uniprot.org/citations/29807321 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/29807321 |
| http://purl.uniprot.org/citations/29807321 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/29807321 |
| http://purl.uniprot.org/uniprot/#_A0A1S5RA93-mappedCitation-29807321 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/29807321 |
| http://purl.uniprot.org/uniprot/#_A0A224AHB5-mappedCitation-29807321 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/29807321 |
| http://purl.uniprot.org/uniprot/#_A0A224APX3-mappedCitation-29807321 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/29807321 |
| http://purl.uniprot.org/uniprot/#_Q96T60-mappedCitation-29807321 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/29807321 |
| http://purl.uniprot.org/uniprot/A0A224APX3 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/29807321 |
| http://purl.uniprot.org/uniprot/A0A224AHB5 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/29807321 |
| http://purl.uniprot.org/uniprot/A0A1S5RA93 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/29807321 |
| http://purl.uniprot.org/uniprot/Q96T60 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/29807321 |