| http://purl.uniprot.org/citations/29853630 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/29853630 | http://www.w3.org/2000/01/rdf-schema#comment | "Vertebrate retinal development follows a pattern during which retinal progenitor cells (RPCs) give rise to all retinal cell types in a highly conserved temporal sequence. RPC proliferation and cell cycle exit are tightly coordinated to ensure proper and timely production of each of the retinal cell types. Extracellular matrix (ECM) plays an important role in eye development, influencing RPC proliferation and differentiation. In this study, we demonstrate that laminins, key ECM components, in the inner limiting membrane, control mitotic spindle orientation by providing environmental cues to the RPCs. In vivo deletion of laminin β2 in mice of both sexes results in a loss RPC basal processes and contact with the ECM, leading to a shift of the mitotic spindle pole orientation toward asymmetric cell divisions. This leads to decreased proliferation and premature RPC pool depletion, resulting in overproduction of rod photoreceptors at the expense of bipolar cells and Müller glia. Moreover, we show that deletion of laminin β2 leads to disruption and mislocalization of its receptors: dystroglycan and β1-integrin. Addition of exogenous β2-containing laminins to laminin β2-deficient retinal explants stabilizes the RPC basal processes and directs their mitotic spindle orientation toward symmetric divisions, leading to increased RPC proliferation, as well as restores proper receptor localization at the retinal surface. Finally, functional blocking of dystroglycan in wild-type retinal explants phenocopies laminin β2 ablation. Our data suggest that dystroglycan-mediated signaling between RPCs and the ECM is of key importance in controlling critical developmental events during retinogenesis.SIGNIFICANCE STATEMENT The mechanisms governing retinogenesis are subject to both intrinsic and extrinsic signaling cues. Although the role of intrinsic signaling has been the subject of many studies, our understanding of the role of the microenvironment in retinal development remains unclear. Using a combination of in vivo and ex vivo approaches, we demonstrate that laminins, key extracellular matrix components, provide signaling cues that control retinal progenitor cell attachment to the basement membrane, mitotic axis, proliferation, and fate adoption. Moreover, we identify, for the first time, dystroglycan as the receptor responsible for directing retinal progenitor cell mitotic spindle orientation. Our data suggest a mechanism where dystroglycan-mediated signaling between the cell and the extracellular matrix controls the proliferative potential of progenitors in the developing CNS."xsd:string |
| http://purl.uniprot.org/citations/29853630 | http://purl.org/dc/terms/identifier | "doi:10.1523/jneurosci.0551-18.2018"xsd:string |
| http://purl.uniprot.org/citations/29853630 | http://purl.uniprot.org/core/author | "Brunken W.J."xsd:string |
| http://purl.uniprot.org/citations/29853630 | http://purl.uniprot.org/core/author | "Hunter D.D."xsd:string |
| http://purl.uniprot.org/citations/29853630 | http://purl.uniprot.org/core/author | "Serjanov D."xsd:string |
| http://purl.uniprot.org/citations/29853630 | http://purl.uniprot.org/core/author | "Bachay G."xsd:string |
| http://purl.uniprot.org/citations/29853630 | http://purl.uniprot.org/core/date | "2018"xsd:gYear |
| http://purl.uniprot.org/citations/29853630 | http://purl.uniprot.org/core/name | "J Neurosci"xsd:string |
| http://purl.uniprot.org/citations/29853630 | http://purl.uniprot.org/core/pages | "5996-6010"xsd:string |
| http://purl.uniprot.org/citations/29853630 | http://purl.uniprot.org/core/title | "Laminin beta2 Chain Regulates Retinal Progenitor Cell Mitotic Spindle Orientation via Dystroglycan."xsd:string |
| http://purl.uniprot.org/citations/29853630 | http://purl.uniprot.org/core/volume | "38"xsd:string |
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