| http://purl.uniprot.org/citations/29883473 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/29883473 | http://www.w3.org/2000/01/rdf-schema#comment | "There is an interest in identifying Anaphase Promoting-Complex/Cyclosome (APC/C) inhibitors that lead to sensitivity to microtubule poisons as a strategy for targeting cancer cells. Using budding yeast Saccharomyces cerevisiae, peptides derived from the Mitotic Arrest Deficient 2 (Mad2)-binding motif of Cell Division Cycle 20 (Cdc20) were observed to inhibit both Cdc20- and CDC20 Homology 1 (Cdh1)-dependent APC/C activity. Over expression of peptides in vivo led to sensitivity to a microtubule poison and, in a recovery from a microtubule poison arrest, delayed degradation of yeast Securin protein Precocious Dissociation of Sisters 1 (Pds1). Peptides with mutations in the Cdc20 activating KILR-motif still bound APC/C, but lost the ability to inhibit APC/C in vitro and lost the ability to induce sensitivity to a microtubule poison in vivo. Thus, an APC/C binding and activation motif that promotes mitotic progression, namely the Cdc20 KILR-motif, can also function as an APC/C inhibitor when present in excess. Another activator for mitotic progression after recovery from microtubule poison is p31comet, where a yeast predicted open-reading frame YBR296C-A encoding a 39 amino acid predicted protein was identified by homology to p31comet, and named Tiny Yeast Comet 1 (TYC1). Tyc1 over expression resulted in sensitivity to microtubule poison. Tyc1 inhibited both APC/CCdc20 and APC/CCdh1 activities in vitro and bound to APC/C. A homologous peptide derived from human p31comet bound to and inhibited yeast APC/C demonstrating evolutionary retention of these biochemical activities. Cdc20 Mad2-binding motif peptides and Tyc1 disrupted the ability of the co-factors Cdc20 and Cdh1 to bind to APC/C, and co-over expression of both together in vivo resulted in an increased sensitivity to microtubule poison. We hypothesize that Cdc20 Mad2-binding motif peptides, Tyc1 and human hp31 peptide can serve as novel molecular tools for investigating APC/C inhibition that leads to sensitivity to microtubule poison in vivo."xsd:string |
| http://purl.uniprot.org/citations/29883473 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pone.0198930"xsd:string |
| http://purl.uniprot.org/citations/29883473 | http://purl.uniprot.org/core/author | "Chen H.Y."xsd:string |
| http://purl.uniprot.org/citations/29883473 | http://purl.uniprot.org/core/author | "Huang A."xsd:string |
| http://purl.uniprot.org/citations/29883473 | http://purl.uniprot.org/core/author | "Chen T.C."xsd:string |
| http://purl.uniprot.org/citations/29883473 | http://purl.uniprot.org/core/author | "Liao L."xsd:string |
| http://purl.uniprot.org/citations/29883473 | http://purl.uniprot.org/core/author | "Schuyler S.C."xsd:string |
| http://purl.uniprot.org/citations/29883473 | http://purl.uniprot.org/core/author | "Lin C.J."xsd:string |
| http://purl.uniprot.org/citations/29883473 | http://purl.uniprot.org/core/author | "Chu Y.T."xsd:string |
| http://purl.uniprot.org/citations/29883473 | http://purl.uniprot.org/core/author | "Cheng V."xsd:string |
| http://purl.uniprot.org/citations/29883473 | http://purl.uniprot.org/core/author | "Tsai W.W."xsd:string |
| http://purl.uniprot.org/citations/29883473 | http://purl.uniprot.org/core/author | "Liao T.W."xsd:string |
| http://purl.uniprot.org/citations/29883473 | http://purl.uniprot.org/core/author | "Wang L.I."xsd:string |
| http://purl.uniprot.org/citations/29883473 | http://purl.uniprot.org/core/author | "Ding Y.S."xsd:string |
| http://purl.uniprot.org/citations/29883473 | http://purl.uniprot.org/core/author | "Wu Y.O."xsd:string |
| http://purl.uniprot.org/citations/29883473 | http://purl.uniprot.org/core/author | "Jhuo J.H."xsd:string |
| http://purl.uniprot.org/citations/29883473 | http://purl.uniprot.org/core/date | "2018"xsd:gYear |
| http://purl.uniprot.org/citations/29883473 | http://purl.uniprot.org/core/name | "PLoS One"xsd:string |
| http://purl.uniprot.org/citations/29883473 | http://purl.uniprot.org/core/pages | "e0198930"xsd:string |
| http://purl.uniprot.org/citations/29883473 | http://purl.uniprot.org/core/title | "Peptide inhibitors of the anaphase promoting-complex that cause sensitivity to microtubule poison."xsd:string |
| http://purl.uniprot.org/citations/29883473 | http://purl.uniprot.org/core/volume | "13"xsd:string |
| http://purl.uniprot.org/citations/29883473 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/29883473 |
| http://purl.uniprot.org/citations/29883473 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/29883473 |
| http://purl.uniprot.org/uniprot/Q8TGU5#attribution-E4A9127CD02592FC38E561BF27AD2B2E | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/29883473 |