http://purl.uniprot.org/citations/29904971 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/29904971 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/29904971 | http://www.w3.org/2000/01/rdf-schema#comment | "BackgroundThe role of sensitization to commercially available allergens of English walnut (Juglans regia) Jug r 1, 2 and 3 in walnut allergy has been previously investigated in walnut allergic adults and was unable to explain all cases of walnut allergy.ObjectivesIdentify recognized walnut allergens, other than the ones previously investigated (Jug r 1-3), in walnut allergic adults and determine the sensitization frequency and diagnostic value.MethodsThree different in-house walnut extracts were prepared and analysed on SDS-PAGE blots to identify allergenic walnut proteins. Immunoblots and immunoprecipitation, followed by LC-MS analysis, were performed to screen for, and confirm, IgE binding to walnut allergens in selected walnut allergic adults. In a cohort of 55 walnut challenged adults, including 33 allergic and 22 tolerant, sensitization to native and recombinant walnut allergen Jug r 4 was assessed using immunoblotting and immuno-line blot (EUROLINE), respectively.ResultsScreening of sera of 8 walnut allergic adults identified Jug r 4 as an allergen in our population. In the total cohort of 55 subjects, 5 were positive for Jug r 4 on immunoblot and 10 on EUROLINE. All but one EUROLINE positive subject had a positive food challenge (sensitivity 27%, specificity 95%, PPV 90%, NPV 47%). All 5 subjects positive on immunoblot were also positive on EUROLINE. LC-MS analysis showed a lack of Jug r 4 in the ImmunoCAP extract. Co-sensitization to other 11S albumins (eg hazelnut Cor a 9) was common in Jug r 4 sensitized subjects, potentially due to cross-reactivity.ConclusionsWalnut 11S globulin Jug r 4 is a relevant minor allergen, recognized by 27% of walnut allergic adults. It has a high positive predictive value of 90% for walnut allergy. Specific IgE against Jug r 4 occurred mostly with concomitant sensitization to other walnut components, mainly Jug r 1."xsd:string |
http://purl.uniprot.org/citations/29904971 | http://purl.org/dc/terms/identifier | "doi:10.1111/cea.13208"xsd:string |
http://purl.uniprot.org/citations/29904971 | http://purl.org/dc/terms/identifier | "doi:10.1111/cea.13208"xsd:string |
http://purl.uniprot.org/citations/29904971 | http://purl.uniprot.org/core/author | "Blankestijn M.A."xsd:string |
http://purl.uniprot.org/citations/29904971 | http://purl.uniprot.org/core/author | "Blankestijn M.A."xsd:string |
http://purl.uniprot.org/citations/29904971 | http://purl.uniprot.org/core/author | "Blom W.M."xsd:string |
http://purl.uniprot.org/citations/29904971 | http://purl.uniprot.org/core/author | "Blom W.M."xsd:string |
http://purl.uniprot.org/citations/29904971 | http://purl.uniprot.org/core/author | "Houben G.F."xsd:string |
http://purl.uniprot.org/citations/29904971 | http://purl.uniprot.org/core/author | "Houben G.F."xsd:string |
http://purl.uniprot.org/citations/29904971 | http://purl.uniprot.org/core/author | "Knulst A.C."xsd:string |
http://purl.uniprot.org/citations/29904971 | http://purl.uniprot.org/core/author | "Knulst A.C."xsd:string |
http://purl.uniprot.org/citations/29904971 | http://purl.uniprot.org/core/author | "Otten H.G."xsd:string |
http://purl.uniprot.org/citations/29904971 | http://purl.uniprot.org/core/author | "Otten H.G."xsd:string |
http://purl.uniprot.org/citations/29904971 | http://purl.uniprot.org/core/author | "de Jong G.A.H."xsd:string |
http://purl.uniprot.org/citations/29904971 | http://purl.uniprot.org/core/author | "de Jong G.A.H."xsd:string |
http://purl.uniprot.org/citations/29904971 | http://purl.uniprot.org/core/author | "Gaspari M."xsd:string |
http://purl.uniprot.org/citations/29904971 | http://purl.uniprot.org/core/author | "Gaspari M."xsd:string |
http://purl.uniprot.org/citations/29904971 | http://purl.uniprot.org/core/author | "Verhoeckx K.C.M."xsd:string |
http://purl.uniprot.org/citations/29904971 | http://purl.uniprot.org/core/author | "Verhoeckx K.C.M."xsd:string |
http://purl.uniprot.org/citations/29904971 | http://purl.uniprot.org/core/author | "den Hartog Jager C.F."xsd:string |
http://purl.uniprot.org/citations/29904971 | http://purl.uniprot.org/core/author | "den Hartog Jager C.F."xsd:string |
http://purl.uniprot.org/citations/29904971 | http://purl.uniprot.org/core/date | "2018"xsd:gYear |
http://purl.uniprot.org/citations/29904971 | http://purl.uniprot.org/core/date | "2018"xsd:gYear |