| http://purl.uniprot.org/citations/29927350 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/29927350 | http://www.w3.org/2000/01/rdf-schema#comment | "CRM1 (Exportin1/XPO1) exports hundreds of broadly functioning protein cargoes out of the cell nucleus by binding to their classical nuclear export signals (NESs). The 8-to 15-amino-acid-long NESs contain four to five hydrophobic residues and are highly diverse in both sequence and CRM1-bound structure. Here we examine the relationship between nuclear export activities of 24 different NES peptides in cells and their CRM1-NES affinities. We found that binding affinity and nuclear export activity are linearly correlated for NESs with dissociation constants ( Kds) between tens of nanomolar to tens of micromolar. NESs with Kds outside this range have significantly reduced nuclear export activities. These include two unusually tight-binding peptides, one from the nonstructural protein 2 of murine minute virus (MVM NS2) and the other a mutant of the protein kinase A inhibitor (PKI) NES. The crystal structure of CRM1-bound MVM NS2NES suggests that extraordinarily tight CRM1 binding arises from intramolecular contacts within the NES that likely stabilizes the CRM1-bound conformation in free peptides. This mechanistic understanding led to the design of two novel peptide inhibitors that bind CRM1 with picomolar affinity."xsd:string |
| http://purl.uniprot.org/citations/29927350 | http://purl.org/dc/terms/identifier | "doi:10.1091/mbc.e18-02-0096"xsd:string |
| http://purl.uniprot.org/citations/29927350 | http://purl.uniprot.org/core/author | "Chook Y.M."xsd:string |
| http://purl.uniprot.org/citations/29927350 | http://purl.uniprot.org/core/author | "Cagatay T."xsd:string |
| http://purl.uniprot.org/citations/29927350 | http://purl.uniprot.org/core/author | "Fu S.C."xsd:string |
| http://purl.uniprot.org/citations/29927350 | http://purl.uniprot.org/core/author | "Fung H.Y.J."xsd:string |
| http://purl.uniprot.org/citations/29927350 | http://purl.uniprot.org/core/author | "Baumhardt J."xsd:string |
| http://purl.uniprot.org/citations/29927350 | http://purl.uniprot.org/core/date | "2018"xsd:gYear |
| http://purl.uniprot.org/citations/29927350 | http://purl.uniprot.org/core/name | "Mol Biol Cell"xsd:string |
| http://purl.uniprot.org/citations/29927350 | http://purl.uniprot.org/core/pages | "2037-2044"xsd:string |
| http://purl.uniprot.org/citations/29927350 | http://purl.uniprot.org/core/title | "Correlation of CRM1-NES affinity with nuclear export activity."xsd:string |
| http://purl.uniprot.org/citations/29927350 | http://purl.uniprot.org/core/volume | "29"xsd:string |
| http://purl.uniprot.org/citations/29927350 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/29927350 |
| http://purl.uniprot.org/citations/29927350 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/29927350 |
| http://purl.uniprot.org/uniprot/#_A0A7I2V2Y6-mappedCitation-29927350 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/29927350 |
| http://purl.uniprot.org/uniprot/#_A0A7I2V461-mappedCitation-29927350 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/29927350 |
| http://purl.uniprot.org/uniprot/#_A0A7I2V6B9-mappedCitation-29927350 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/29927350 |
| http://purl.uniprot.org/uniprot/#_B3KWD0-mappedCitation-29927350 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/29927350 |
| http://purl.uniprot.org/uniprot/#_B4DR01-mappedCitation-29927350 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/29927350 |
| http://purl.uniprot.org/uniprot/#_B7ZB16-mappedCitation-29927350 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/29927350 |
| http://purl.uniprot.org/uniprot/#_P41920-mappedCitation-29927350 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/29927350 |
| http://purl.uniprot.org/uniprot/#_P62826-mappedCitation-29927350 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/29927350 |
| http://purl.uniprot.org/uniprot/#_O14980-mappedCitation-29927350 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/29927350 |
| http://purl.uniprot.org/uniprot/#_P0DJZ2-mappedCitation-29927350 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/29927350 |