| http://purl.uniprot.org/citations/29953592 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/29953592 | http://www.w3.org/2000/01/rdf-schema#comment | "Mammalian sperm must undergo a functionally defined process called capacitation to be able to fertilize oocytes. They become capacitated in vivo by interacting with the female reproductive tract or in vitro in a defined capacitation medium that contains bovine serum albumin, calcium (Ca2+ ), and bicarbonate (HCO3- ). In this work, sperm were double stained with propidium iodide and the Ca2+ dye Fluo-4 AM and analyzed by flow cytometry to determine changes in intracellular Ca2+ concentration ([Ca2+ ]i ) in individual live sperm. An increase in [Ca2+ ]i was observed in a subpopulation of capacitated live sperm when compared with noncapacitated ones. Sperm exposed to the capacitating medium displayed a rapid increase in [Ca2+ ]i within 1 min of incubation, which remained sustained for 90 min. These rise in [Ca2+ ]i after 90 min of incubation in the capacitating medium was evidenced by an increase in the normalized median fluorescence intensity. This increase was dependent on the presence of extracellular Ca2+ and, at least in part, reflected the contribution of a new subpopulation of sperm with higher [Ca2+ ]i . In addition, it was determined that the capacitation-associated [Ca2+ ]i increase was dependent of CatSper channels, as sperm derived from CatSper knockout (CatSper KO) or incubated in the presence of CatSper inhibitors failed to increase [Ca2+ ]i . Surprisingly, a minimum increase in [Ca2+ ]i was also observed in CatSper KO sperm suggesting the existence of other Ca2+ transport systems. Altogether, these results indicate that a subpopulation of sperm increases [Ca2+ ]i very rapidly during capacitation mainly due to a CatSper-mediated influx of extracellular Ca2+ ."xsd:string |
| http://purl.uniprot.org/citations/29953592 | http://purl.org/dc/terms/identifier | "doi:10.1002/jcp.26883"xsd:string |
| http://purl.uniprot.org/citations/29953592 | http://purl.uniprot.org/core/author | "Visconti P.E."xsd:string |
| http://purl.uniprot.org/citations/29953592 | http://purl.uniprot.org/core/author | "Buffone M.G."xsd:string |
| http://purl.uniprot.org/citations/29953592 | http://purl.uniprot.org/core/author | "Puga Molina L.C."xsd:string |
| http://purl.uniprot.org/citations/29953592 | http://purl.uniprot.org/core/author | "Romarowski A."xsd:string |
| http://purl.uniprot.org/citations/29953592 | http://purl.uniprot.org/core/author | "Krapf D."xsd:string |
| http://purl.uniprot.org/citations/29953592 | http://purl.uniprot.org/core/author | "Martin-Hidalgo D."xsd:string |
| http://purl.uniprot.org/citations/29953592 | http://purl.uniprot.org/core/author | "Luque G.M."xsd:string |
| http://purl.uniprot.org/citations/29953592 | http://purl.uniprot.org/core/author | "Dalotto-Moreno T."xsd:string |
| http://purl.uniprot.org/citations/29953592 | http://purl.uniprot.org/core/author | "Balestrini P.A."xsd:string |
| http://purl.uniprot.org/citations/29953592 | http://purl.uniprot.org/core/author | "Gilio N."xsd:string |
| http://purl.uniprot.org/citations/29953592 | http://purl.uniprot.org/core/author | "Ritagliati C."xsd:string |
| http://purl.uniprot.org/citations/29953592 | http://purl.uniprot.org/core/author | "Schiavi-Ehrenhaus L.J."xsd:string |
| http://purl.uniprot.org/citations/29953592 | http://purl.uniprot.org/core/date | "2018"xsd:gYear |
| http://purl.uniprot.org/citations/29953592 | http://purl.uniprot.org/core/name | "J Cell Physiol"xsd:string |
| http://purl.uniprot.org/citations/29953592 | http://purl.uniprot.org/core/pages | "9685-9700"xsd:string |
| http://purl.uniprot.org/citations/29953592 | http://purl.uniprot.org/core/title | "Only a subpopulation of mouse sperm displays a rapid increase in intracellular calcium during capacitation."xsd:string |
| http://purl.uniprot.org/citations/29953592 | http://purl.uniprot.org/core/volume | "233"xsd:string |
| http://purl.uniprot.org/citations/29953592 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/29953592 |
| http://purl.uniprot.org/citations/29953592 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/29953592 |
| http://purl.uniprot.org/uniprot/#_A0A060IFK2-mappedCitation-29953592 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/29953592 |
| http://purl.uniprot.org/uniprot/#_A0A060IKF0-mappedCitation-29953592 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/29953592 |
| http://purl.uniprot.org/uniprot/#_A0A060ILI2-mappedCitation-29953592 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/29953592 |