| http://purl.uniprot.org/citations/29959925 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/29959925 | http://www.w3.org/2000/01/rdf-schema#comment | "It has long been suggested that chromatin may form a fiber with a diameter of ~30 nm that suppresses transcription. Despite nearly four decades of study, the structural nature of the 30-nm chromatin fiber and conclusive evidence of its existence in vivo remain elusive. The key support for the existence of specific 30-nm chromatin fiber structures is based on the determination of the structures of reconstituted nucleosome arrays using X-ray crystallography and single-particle cryo-electron microscopy coupled with glutaraldehyde chemical cross-linking. Here we report the characterization of these nucleosome arrays in solution using analytical ultracentrifugation, NMR, and small-angle X-ray scattering. We found that the physical properties of these nucleosome arrays in solution are not consistent with formation of just a few discrete structures of nucleosome arrays. In addition, we obtained a crystal of the nucleosome in complex with the globular domain of linker histone H5 that shows a new form of nucleosome packing and suggests a plausible alternative compact conformation for nucleosome arrays. Taken together, our results challenge the key evidence for the existence of a limited number of structures of reconstituted nucleosome arrays in solution by revealing that the reconstituted nucleosome arrays are actually best described as an ensemble of various conformations with a zigzagged arrangement of nucleosomes. Our finding has implications for understanding the structure and function of chromatin in vivo."xsd:string |
| http://purl.uniprot.org/citations/29959925 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.jmb.2018.06.020"xsd:string |
| http://purl.uniprot.org/citations/29959925 | http://purl.uniprot.org/core/author | "Bai Y."xsd:string |
| http://purl.uniprot.org/citations/29959925 | http://purl.uniprot.org/core/author | "Feng H."xsd:string |
| http://purl.uniprot.org/citations/29959925 | http://purl.uniprot.org/core/author | "Jiang J."xsd:string |
| http://purl.uniprot.org/citations/29959925 | http://purl.uniprot.org/core/author | "Ghirlando R."xsd:string |
| http://purl.uniprot.org/citations/29959925 | http://purl.uniprot.org/core/author | "Zhang P."xsd:string |
| http://purl.uniprot.org/citations/29959925 | http://purl.uniprot.org/core/author | "Wang R."xsd:string |
| http://purl.uniprot.org/citations/29959925 | http://purl.uniprot.org/core/author | "Zhou B.R."xsd:string |
| http://purl.uniprot.org/citations/29959925 | http://purl.uniprot.org/core/author | "Sathish Yadav K.N."xsd:string |
| http://purl.uniprot.org/citations/29959925 | http://purl.uniprot.org/core/author | "Norouzi D."xsd:string |
| http://purl.uniprot.org/citations/29959925 | http://purl.uniprot.org/core/author | "Zhurkin V."xsd:string |
| http://purl.uniprot.org/citations/29959925 | http://purl.uniprot.org/core/date | "2018"xsd:gYear |
| http://purl.uniprot.org/citations/29959925 | http://purl.uniprot.org/core/name | "J Mol Biol"xsd:string |
| http://purl.uniprot.org/citations/29959925 | http://purl.uniprot.org/core/pages | "3093-3110"xsd:string |
| http://purl.uniprot.org/citations/29959925 | http://purl.uniprot.org/core/title | "Revisit of Reconstituted 30-nm Nucleosome Arrays Reveals an Ensemble of Dynamic Structures."xsd:string |
| http://purl.uniprot.org/citations/29959925 | http://purl.uniprot.org/core/volume | "430"xsd:string |
| http://purl.uniprot.org/citations/29959925 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/29959925 |
| http://purl.uniprot.org/citations/29959925 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/29959925 |
| http://purl.uniprot.org/uniprot/#_P02299-mappedCitation-29959925 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/29959925 |
| http://purl.uniprot.org/uniprot/#_P02259-mappedCitation-29959925 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/29959925 |
| http://purl.uniprot.org/uniprot/#_P02283-mappedCitation-29959925 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/29959925 |
| http://purl.uniprot.org/uniprot/#_P84040-mappedCitation-29959925 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/29959925 |
| http://purl.uniprot.org/uniprot/#_P84051-mappedCitation-29959925 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/29959925 |