| http://purl.uniprot.org/citations/29970602 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/29970602 | http://www.w3.org/2000/01/rdf-schema#comment | "Soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) family proteins mediate membrane fusion critical for vesicular transport and cellular secretion. Mast cells rely on SNARE-mediated membrane fusion for degranulation stimulated by crosslinking of immunoglobulin E (IgE) bound to the Fcε receptor (FcεRI). We investigated the mechanisms downstream of receptor activation that control degranulation. We found that the SNARE binding protein tomosyn-1 (also known as STXBP5) inhibited FcεRI-stimulated degranulation of mast cells. After mast cell activation, tomosyn-1 was phosphorylated on serine and threonine residues, dissociated from the SNARE protein syntaxin 4 (STX4), and associated with STX3. We identified PKCδ as the major kinase required for tomosyn-1 threonine phosphorylation and for regulation of the interaction with STXs. Incubation with high IgE concentrations increased tomosyn-1 abundance in cultured mast cells. Similarly, in basophils from allergic patients with high amounts of serum IgE, the abundance of tomosyn-1 was increased as compared to that in patients with normal IgE concentrations. Our findings identified tomosyn-1 as an inhibitor of mast cell degranulation that required PKCδ to switch its interaction with STX partners during fusion. We suggest that the IgE-mediated increase in tomosyn-1 abundance in allergic patients may represent a counterregulatory mechanism to limit disease development."xsd:string |
| http://purl.uniprot.org/citations/29970602 | http://purl.org/dc/terms/identifier | "doi:10.1126/scisignal.aan4350"xsd:string |
| http://purl.uniprot.org/citations/29970602 | http://purl.uniprot.org/core/author | "Tiwari N."xsd:string |
| http://purl.uniprot.org/citations/29970602 | http://purl.uniprot.org/core/author | "Gonzalez-Espinosa C."xsd:string |
| http://purl.uniprot.org/citations/29970602 | http://purl.uniprot.org/core/author | "Macias-Silva M."xsd:string |
| http://purl.uniprot.org/citations/29970602 | http://purl.uniprot.org/core/author | "Klingebiel C."xsd:string |
| http://purl.uniprot.org/citations/29970602 | http://purl.uniprot.org/core/author | "Vitte J."xsd:string |
| http://purl.uniprot.org/citations/29970602 | http://purl.uniprot.org/core/author | "Blank U."xsd:string |
| http://purl.uniprot.org/citations/29970602 | http://purl.uniprot.org/core/author | "Menasche G."xsd:string |
| http://purl.uniprot.org/citations/29970602 | http://purl.uniprot.org/core/author | "Benhamou M."xsd:string |
| http://purl.uniprot.org/citations/29970602 | http://purl.uniprot.org/core/author | "Charles N."xsd:string |
| http://purl.uniprot.org/citations/29970602 | http://purl.uniprot.org/core/author | "Dema B."xsd:string |
| http://purl.uniprot.org/citations/29970602 | http://purl.uniprot.org/core/author | "Danelli L."xsd:string |
| http://purl.uniprot.org/citations/29970602 | http://purl.uniprot.org/core/author | "Madera-Salcedo I.K."xsd:string |
| http://purl.uniprot.org/citations/29970602 | http://purl.uniprot.org/core/author | "Vibhushan S."xsd:string |
| http://purl.uniprot.org/citations/29970602 | http://purl.uniprot.org/core/author | "Pacreau E."xsd:string |
| http://purl.uniprot.org/citations/29970602 | http://purl.uniprot.org/core/author | "Agabriel C."xsd:string |
| http://purl.uniprot.org/citations/29970602 | http://purl.uniprot.org/core/author | "Birnbaum J."xsd:string |
| http://purl.uniprot.org/citations/29970602 | http://purl.uniprot.org/core/author | "Liabeuf V."xsd:string |
| http://purl.uniprot.org/citations/29970602 | http://purl.uniprot.org/core/date | "2018"xsd:gYear |
| http://purl.uniprot.org/citations/29970602 | http://purl.uniprot.org/core/name | "Sci Signal"xsd:string |
| http://purl.uniprot.org/citations/29970602 | http://purl.uniprot.org/core/pages | "eaan4350"xsd:string |
| http://purl.uniprot.org/citations/29970602 | http://purl.uniprot.org/core/title | "Tomosyn functions as a PKCdelta-regulated fusion clamp in mast cell degranulation."xsd:string |
| http://purl.uniprot.org/citations/29970602 | http://purl.uniprot.org/core/volume | "11"xsd:string |