| http://purl.uniprot.org/citations/30012438 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/30012438 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/30012438 | http://www.w3.org/2000/01/rdf-schema#comment | "Integrons are genetic platforms responsible for the dissemination of antimicrobial resistance genes among Gram-negative bacteria, primarily due to their association with transposable elements and conjugative plasmids. In this study, a cassette array containing four identical blaGES-5 genes embedded in a class 1 integron located on an IncP-1β group plasmid from a clinical Pseudomonas aeruginosa strain was identified. Comparative genome analysis and conjugation assay showed that the plasmid pICP-4GES lacked the trbN, trbO and trbP genes but was conjugable. Antimicrobial susceptibility testing revealed that compared with single-copy blaGES-5 complementary strains, both the cloned and chromosome-targeted expression of four copies of blaGES-5 increased the minimum inhibitory concentration (MIC) by one to two dilutions for most of the selected antimicrobials. Quantitative real-time reverse transcription PCR (RT-qPCR) showed that the four consecutive cassettes increased blaGES-5 expression by approximately two-fold compared with the single-copy blaGES-5 strain, suggesting that the level of gene expression was not directly proportional to copy number. In addition, the gene cassette capture assay showed that the global blaGES-5 transfer frequency reached 5.38 × 10-4."xsd:string |
| http://purl.uniprot.org/citations/30012438 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.ijantimicag.2018.07.002"xsd:string |
| http://purl.uniprot.org/citations/30012438 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.ijantimicag.2018.07.002"xsd:string |
| http://purl.uniprot.org/citations/30012438 | http://purl.uniprot.org/core/author | "Bao Q."xsd:string |
| http://purl.uniprot.org/citations/30012438 | http://purl.uniprot.org/core/author | "Bao Q."xsd:string |
| http://purl.uniprot.org/citations/30012438 | http://purl.uniprot.org/core/author | "Hu Y."xsd:string |
| http://purl.uniprot.org/citations/30012438 | http://purl.uniprot.org/core/author | "Hu Y."xsd:string |
| http://purl.uniprot.org/citations/30012438 | http://purl.uniprot.org/core/author | "Liu Y."xsd:string |
| http://purl.uniprot.org/citations/30012438 | http://purl.uniprot.org/core/author | "Liu Y."xsd:string |
| http://purl.uniprot.org/citations/30012438 | http://purl.uniprot.org/core/author | "Lu J."xsd:string |
| http://purl.uniprot.org/citations/30012438 | http://purl.uniprot.org/core/author | "Lu J."xsd:string |
| http://purl.uniprot.org/citations/30012438 | http://purl.uniprot.org/core/author | "Li K."xsd:string |
| http://purl.uniprot.org/citations/30012438 | http://purl.uniprot.org/core/author | "Li K."xsd:string |
| http://purl.uniprot.org/citations/30012438 | http://purl.uniprot.org/core/author | "Li P."xsd:string |
| http://purl.uniprot.org/citations/30012438 | http://purl.uniprot.org/core/author | "Li P."xsd:string |
| http://purl.uniprot.org/citations/30012438 | http://purl.uniprot.org/core/author | "Xu L."xsd:string |
| http://purl.uniprot.org/citations/30012438 | http://purl.uniprot.org/core/author | "Xu L."xsd:string |
| http://purl.uniprot.org/citations/30012438 | http://purl.uniprot.org/core/author | "Wang J."xsd:string |
| http://purl.uniprot.org/citations/30012438 | http://purl.uniprot.org/core/author | "Wang J."xsd:string |
| http://purl.uniprot.org/citations/30012438 | http://purl.uniprot.org/core/author | "Xu T."xsd:string |
| http://purl.uniprot.org/citations/30012438 | http://purl.uniprot.org/core/author | "Xu T."xsd:string |
| http://purl.uniprot.org/citations/30012438 | http://purl.uniprot.org/core/author | "Yi H."xsd:string |
| http://purl.uniprot.org/citations/30012438 | http://purl.uniprot.org/core/author | "Yi H."xsd:string |