Results

RDF/XMLNTriplesTurtleShow queryShare ▶
SubjectPredicateObject
http://purl.uniprot.org/citations/30021515http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/30021515http://www.w3.org/2000/01/rdf-schema#comment"

Background

Interleukin 2 (IL-2) influences the development and severity of pain due to its antinociceptive and immunomodulatory effects. Its production is influenced by the increased expression of c-Cbl (Casitas B-lineage lymphoma proto-oncogene) and Cbl-b E3 ubiquitin ligases. We evaluated the effects on IL-2-mediated changes in c-Cbl and Cbl-b expression in a rat model of chronic neuropathic pain.

Methods

Peripheral neuropathy was induced in adult male Sprague-Dawley rats weighing 250-300 g by chronic spinal nerve ligation. Half of the spinal cord ipsilateral to the nerve injury was harvested at 1, 3, and 6 weeks, and the expression levels of IL-2, c-Cbl, Cbl-b, phospholipase C-γ1 (PLC-γ1), ZAP70, and protein kinase Cθ (PKCθ), as well as ubiquitin conjugation, were evaluated.

Results

Total IL-2 mRNA levels were significantly decreased at 3 and 6 weeks after nerve injury compared to those in sham-operated rats. The mRNA levels of c-Cbl and Cbl-b, as well as the level of ubiquitin conjugation, were significantly increased at 3 and 6 weeks. In contrast, the levels of phosphorylated ZAP70 and PLC-γ1 were decreased at 3 and 6 weeks after spinal nerve ligation. Ubiquitination of PLC-γ1 and PKCθ was increased at 3 and 6 weeks.

Conclusions

Our results suggest that ubiquitin and the E3 ubiquitin ligases c-Cbl and Cbl-b function as neuroimmune modulators in the subacute phase of neuropathic pain after nerve injury."xsd:string
http://purl.uniprot.org/citations/30021515http://purl.org/dc/terms/identifier"doi:10.1186/s12871-018-0555-z"xsd:string
http://purl.uniprot.org/citations/30021515http://purl.uniprot.org/core/author"Yoon J.H."xsd:string
http://purl.uniprot.org/citations/30021515http://purl.uniprot.org/core/author"Jeong J.S."xsd:string
http://purl.uniprot.org/citations/30021515http://purl.uniprot.org/core/author"Lee A.R."xsd:string
http://purl.uniprot.org/citations/30021515http://purl.uniprot.org/core/author"Lee J.E."xsd:string
http://purl.uniprot.org/citations/30021515http://purl.uniprot.org/core/author"Kim H.Y."xsd:string
http://purl.uniprot.org/citations/30021515http://purl.uniprot.org/core/author"Shin B.S."xsd:string
http://purl.uniprot.org/citations/30021515http://purl.uniprot.org/core/author"Hahm T.S."xsd:string
http://purl.uniprot.org/citations/30021515http://purl.uniprot.org/core/date"2018"xsd:gYear
http://purl.uniprot.org/citations/30021515http://purl.uniprot.org/core/name"BMC Anesthesiol"xsd:string
http://purl.uniprot.org/citations/30021515http://purl.uniprot.org/core/pages"87"xsd:string
http://purl.uniprot.org/citations/30021515http://purl.uniprot.org/core/title"Increased expression of the Cbl family of E3 ubiquitin ligases decreases Interleukin-2 production in a rat model of peripheral neuropathy."xsd:string
http://purl.uniprot.org/citations/30021515http://purl.uniprot.org/core/volume"18"xsd:string
http://purl.uniprot.org/citations/30021515http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/30021515
http://purl.uniprot.org/citations/30021515http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/30021515
http://purl.uniprot.org/uniprot/#_A0A0G2K8K2-mappedCitation-30021515http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/30021515
http://purl.uniprot.org/uniprot/#_A0A0U1ZEI3-mappedCitation-30021515http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/30021515
http://purl.uniprot.org/uniprot/#_A0A0U1ZF16-mappedCitation-30021515http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/30021515
http://purl.uniprot.org/uniprot/#_A0A0U1ZFN5-mappedCitation-30021515http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/30021515
http://purl.uniprot.org/uniprot/#_D3ZV15-mappedCitation-30021515http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/30021515
http://purl.uniprot.org/uniprot/#_A0A8I5ZWU2-mappedCitation-30021515http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/30021515
http://purl.uniprot.org/uniprot/#_A6J3W2-mappedCitation-30021515http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/30021515
http://purl.uniprot.org/uniprot/#_A0A8I6G3R2-mappedCitation-30021515http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/30021515
▶