| http://purl.uniprot.org/citations/30061199 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/30061199 | http://www.w3.org/2000/01/rdf-schema#comment | "The C/EBPα transcription factor is required for myelopoiesis, with prior observations suggesting additional contributions to B lymphopoiesis. Cebpa expression is evident in common lymphoid progenitor (CLP) and preproB cells but is absent in proB and preB cells. We previously observed that marrow lacking the Cebpa +37 kb enhancer is impaired in producing B cells upon competitive transplantation. Additionally, a Cebpa enhancer/promoter-hCD4 transgene is expressed in B/myeloid CFU. Extending these findings, pan-hematopoietic murine Cebpa enhancer deletion using Mx1-Cre leads to expanded CLP, fewer preproB cells, markedly reduced proB and preB cells, and reduced mature B cells, without affecting T cell numbers. In contrast, enhancer deletion at the proB stage using Mb1-Cre does not impair B cell maturation. Further evaluation of CLP reveals that the Cebpa transgene is expressed almost exclusively in Flt3+ multipotent CLP versus B cell-restricted Flt3- CLP. In vitro, hCD4+ preproB cells produce both B and myeloid cells, whereas hCD4- preproB cells only produce B cells. Additionally, a subset of hCD4- preproB cells express high levels of RAG1-GFP, as seen also in proB cells. Global gene expression analysis indicates that hCD4+ preproB cells express proliferative pathways, whereas B cell development and signal transduction pathways predominate in hCD4- preproB cells. Consistent with these changes, Cebpa enhancer-deleted preproB cells downmodulate cell cycle pathways while upregulating B cell signaling pathways. Collectively, these findings indicate that C/EBPα is required for Flt3+ CLP maturation into preproB cells and then for proliferative Cebpaint B/myeloid preproB cells to progress to Cebpalo B cell-restricted preproB cells and finally to Cebpaneg proB cells."xsd:string |
| http://purl.uniprot.org/citations/30061199 | http://purl.org/dc/terms/identifier | "doi:10.4049/jimmunol.1800244"xsd:string |
| http://purl.uniprot.org/citations/30061199 | http://purl.uniprot.org/core/author | "Guo H."xsd:string |
| http://purl.uniprot.org/citations/30061199 | http://purl.uniprot.org/core/author | "Friedman A.D."xsd:string |
| http://purl.uniprot.org/citations/30061199 | http://purl.uniprot.org/core/author | "Suresh R."xsd:string |
| http://purl.uniprot.org/citations/30061199 | http://purl.uniprot.org/core/author | "Barberi T."xsd:string |
| http://purl.uniprot.org/citations/30061199 | http://purl.uniprot.org/core/date | "2018"xsd:gYear |
| http://purl.uniprot.org/citations/30061199 | http://purl.uniprot.org/core/name | "J Immunol"xsd:string |
| http://purl.uniprot.org/citations/30061199 | http://purl.uniprot.org/core/pages | "1692-1704"xsd:string |
| http://purl.uniprot.org/citations/30061199 | http://purl.uniprot.org/core/title | "Progression from the Common Lymphoid Progenitor to B/Myeloid PreproB and ProB Precursors during B Lymphopoiesis Requires C/EBPalpha."xsd:string |
| http://purl.uniprot.org/citations/30061199 | http://purl.uniprot.org/core/volume | "201"xsd:string |
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